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  • 1
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract L-Tartaric acid was produced by transformation of cis-epoxysuccinic acid using Nocardia tartaricans ATCC 31191 in a molar yield of 100%. cis-Epoxysuccinic acid was prepared by epoxidation of maleic acid in the presence of molybdate or tungstate ions. The effect of these ions on the subsequent activity of the cis-epoxysuccinate hydrolase was determined in both intact cells and cell-free extracts of Nocardia tartaricans. Based on kinetic studies, the tungstate ions behaved as a strong inhibitor while the molybdate ions as a stimulator of the enzyme. © Rapid Science Ltd. 1998
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biotechnology letters 21 (1999), S. 491-495 
    ISSN: 1573-6776
    Keywords: bioconversion ; cis-epoxysuccinic acid ; immobilization ; Nocardia ; tartaric acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Nocardia tartaricans converted sodium cis-epoxysuccinate to L-tartrate. The highest cis-epoxysuccinate hydrolase activity (37.7 U mg−1) was obtained with 0.02% (w/v) sodium deoxycholate, but this inactivated the cells. Immobilized N. tartaricans in pectate gel showed higher enzyme activity (51 U mg −1) compare to the free cells (8.9 U mg −1). After 450 days, the immobilized cells still possessed 0.65 U mg −1, i.e. 30% of the initial enzyme activity.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 19 (1999), S. 357-363 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: An alternative microbiological method for the production of malate from fumarate is presented. The yeast Dipodascus magnusii was used for this bioconversion.The optimum cell growth temperature was 28°C and the working volume 120 ml. The highest level of fumarase activity during bioconversion was achieved at a pH of 7.5 and a temperature of 37°C. These conditions were determined as optimal. Using sodium fumarate (1M), the maximum specific productivity of malic acid obtained was 1.72 g/(gDCW × h) for intact cells. In the case of ammonium fumarate, it was 2.25 g/(gDCW × h).
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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