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  • 1
    ISSN: 1432-0568
    Keywords: Key words Birds ; Confocal laser scanning microscopy ; Urinary tract ; Tyrosine hydroxylase ; Vasoactive intestinal peptide.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The distribution and colocalisation of nicotinamide adenine dinucleotide phosphate reduced-diaphorase (NADPH-d)-/nitric oxide synthase (NOS)-containing (nitrergic) neurons in the innervation of the duck ureter have been studied using histochemistry and immunohistochemistry. Quantitative analysis showed that nitrergic neurons made up 60% and 70% of the total intramural and adventitial neuronal populations, respectively. About 40% of intramural nitrergic neurons expressed VIP-immunoreactivity, and about 75% of nitrergic adventitial neurons expressed TH-immunoreactivity. The density of nitrergic adventitial neurons was significantly greater in the lower tract than in the upper and intermediate tracts. Nerve lesioning experiments showed that the majority of ureteral nitrergic innervation was extrinsic in origin; nitrergic adventitial neurons primarily projected caudocranially, whereas NOS-immunoreactive and NOS-/VIP-immunoreactive intramural neurons primarily projected craniocaudally. These findings suggest that, in birds, the nitrergic innervation plays a role in ureteral functions such as epithelial mucosecretion, muscular motility, and the closing and/or opening of the ureteral papilla.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 24 (1995), S. 0 
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The morpho-functional and topographical features of the lingual papillae situated on the dorsal surface of the bovine tongue, were studied utilizing LM and SEM techniques. In the bovine species, the functional differentiation of the lingual papillae in their gustative and mechanical modes, seems to be related to the position of the papillae rather than to their morphological features. The gustative function predominates over the mechanical one on the caudal tract of the tongue body (lateral to the lingual torus and associated with circumvallate papillae). The gustative function also involves the conical papillae situated on the caudal tract of the tongue body. This gustative function is aided by additional anatomical structures: 1. The grooves situated on the rostro-lateral side of the conical papillae; 2. The microcraters located on the top of the fungiform papillae; and 3. The furrows of the circumvallatae papillae vallum. The contact between saliva soluble food particles and taste buds is aided and made more efficient by these structures. Furthermore, a large variety of conical papillae were observed, whereas no filiform papillae were noted.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 23 (1994), S. 0 
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The goat choroid plexus angioarchitecture of the lateral ventricles was studied under the SEM using the method of “microvascular corrosion casts”. The whole plexus is semilunar shaped and directed in an antero-posterior, latero-median fashion. In the plexus the lateral extremity is larger than the median one. All the components of the vascular bed (arteries, venes and capillaries) of the choroid plexus have interesting morpho-structural features. In particular, the capillaries are more developed than the other components and they are variously located on both sides of the plexus. The capillary network has a various organization in different zones of each side of the plexus.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Introduction:  Agouti-related protein (AGRP) is a neuropeptide involved in the control of body weight. Morphological and pharmacological studies have shown that AGRP is implicated in the central control of feeding behaviour acting as an endogenous antagonist of the alpha-melanocyte stimulating hormone (α-MSH), a potent satiety-inducing factor, at the melanocortin 3 (MC3)- and four (MC4)-receptors.Aim:  The aim of the present study was to investigate the expression of AGRP and its mRNA in the hypothalamus and adrenal gland of the duck and, in particular, to establish which type of adrenal tissue is involved in the AGRP synthesis.Methods:  Immunohistochemistry, western blotting, reverse transcriptase (RT)-polimerase chain reaction (PCR).Results and Discussion:  AGRP-immunoreactivity was observed in neurons and nerve fibres in a restricted area of the hypothalamus. AGRP-ir neurons were located in the nucleus infundibularis and distributed ventromedially to the third ventricle in the hypothalamic tuberal region. These neurons were round or, with a lesser extent, elongated in shape. AGRP-ir fibres were seen to project to the median eminence (ME) and anterior periventricular hypothalamus. The AGRP ir-fibres in the ME were distributed in the external layer in close vicinity to the capillaries of the hypothalamo-hypophysial portal system. In the avian adrenal gland, AGRP immunoreactivity was observed in the medullary tissue. A partial sequence of AGRP cDNA was identified using RT-PCR cloning and sequencing. This sequence was highly homologous to the corresponding fragment of the chicken AGRP gene. The western blotting analysis of adrenal gland and hypothalamus tissue extracts showed a well-defined single band with an electrophoretic mobility consistent with the molecular weight of the avian AGRP protein. These results demonstrate that AGRP is expressed in the hypothalamus and adrenal glands of the duck and suggest an involvement of this peptide in the regulation of the melanocortin system in birds.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 34 (2005), S. 0 
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Introduction:  Neurotrophic factors constitute a group of growth factor families, which have important effects on survival and differentiation of neuronal cells. The neurotrophin family is composed of nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin (NT)3 and NT 4/5. Neurotrophins act by means of high (TrkA, TrkB and TrkC) and low (p75) affinity receptors on numerous neuronal populations of central and peripheral nervous system. The family of glial derived neurotrophic factor (GDNF) includes, besides the GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). They bind to a common receptor Ret, but the binding specificity is due to a group of proteins (GFRα 1–4). These factors show a trophic effect on dorsal ganglia, motor neurons and autonomic nervous system. The aim of the present study is to evaluate the expression of NGF, BDNF and GDNF in the vas deferens and accessory genital glands of normal and castrated rats.Methods:  Immunohistochemistry, enzyme linked immunoassay (ELISA), reverse transcriptase (RT)-polymerase chain reaction (PCR).Results and Discussion:  Immunoreactivity to NGF, BDNF and GDNF was observed in all the investigated tracts. Generally, this immunoreactivity seemed to increase in castrated rats. ELISA and RT-PCR were performed to evaluate the levels of BDNF protein and its mRNA. In the normals, the greatest concentration of BDNF was observed in the vesicular gland, the lowest in the prostate. In the castrated, the BDNF concentration significantly decreased in the vas deferens. Conversely, it increased in the vesicular gland and in the ventral and dorsal prostate. BDNF transcripts were detected in both normal and castrated rats. These results suggest that neurotrophic factors are produced by the vas deferens and accessory genital glands and, in normal conditions, they are downregulated by androgens.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 34 (2005), S. 0 
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Introduction:  The interactions occurring between nervous and immune systems are well documented. These interactions involve several types of chemical messengers including hormones, cytokines, classic neurotransmitters and neuropeptides. It has been observed that the lymphoid organs receive a dense peptidergic innervation and immune cells produce neuropeptides. Neuropeptides, in turn, are involved in the regulation of the inflammatory processes and in the maturation of the lymphoid organs. Several studies have demonstrated that the immunomodulatory neuropeptides and their receptors are expressed in the thymus and bursa of fabricius. PACAP is a glucagon/VIP/secretin family peptide. It was originally isolated from the ovine hypothalamus and then it was found in the autonomic nervous system. PACAP is involved in the regulation of the hypothalamic-pituitary function, neurotransmission and neuromodulation. In the immune system, PACAP is expressed in lymphoid tissues of the rat and in the lymphocytes of the duck GALT. PACAP, therefore, could be a messenger of the dialogue between nervous and immune system. It may have a role in the regulation of the inflammatory processes by stimulating histamine and serotonin and modulating the production of the cytokines in immune cells.Methods:  Immunohistochemistry on paraffin-embedded sections of thymus and bursa of fabricius of the duck of different ages by using an antibody anti-PACAP38.Results and Discussion:  In the thymus, PACAP-immunoreactivity was found in lymphoid cells and, with a lesser extent, in epithelial reticular cells. The immunoreactive lymphocytes were primarily observed in the interlobular septa in close vicinity to the interlobular veins. The number of positive lymphocytes increased with ageing. In the bursa of fabricius, PACAP-IR was found in nerve fibres and in a few lymphoid cells. These results suggest that PACAP could play a role in the maturation and involution of these organs and in the immune functions.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 27 (1998), S. 0 
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The morphology of the ureter of the duck was investigated, using histological, SEM and TEM techniques. The inner perimeter, the total thickness of the ureteral wall and the thickness of each uretral layer were measured. The epithelium was tall columnar and pseudostratified along all the tracts of the ureter and showed a high muco-secretive activity. The lamina propria contained numerous capillaries and aggregates of leucocytes and macrophages. Throughout the lamina propria there was a dense plexus of nerves. Some denuded single nerve fibres were observed between the epithelial basal cells. A plexus of nerves was also observed in the tunica muscularis. The ostium cloacale ureteris opened on a well-developed papilla in the dorsal region of the urodeum. The total thickness of the ureteral wall, the thickness of the lamina propria and the tunica muscularis, and the inner perimeter progressively increased towards the ostium. The above observations suggest that the avian ureter plays an important role in the modification of the urine coming from medullary cones, and in emission of the urine into the cloaca.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Lung 138 (1968), S. 227-228 
    ISSN: 1432-1750
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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