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  • 1
    Electronic Resource
    Electronic Resource
    Prostaglandin synthesis by the cochlea
    Amsterdam : Elsevier
    Prostaglandins 35 (1988), S. 173-184 
    Details
    ISSN: 0090-6980
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0090-6980(88)90085-8
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  • 2
    Electronic Resource
    Electronic Resource
    Primary reconstruction by modified cervical island skin flap following resection of oral and pharyngeal cancer (1978)
    Springer
    European archives of oto-rhino-laryngology and head & neck 221 (1978), S. 203-210 
    Details
    ISSN: 1434-4726
    Keywords: Primary reconstruction ; Modified island skin flap ; Intraoral defect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Reconstruction of the intraoral defect following cancer surgery often causes formation of fistula, impairment of lingual movement, and prolonged hospitalization by secondary reconstruction. To reduce such complications, the modified cervical island skin flap method as a one-stage intraoral reconstruction was used in 18 patients. Lateral cervical island flap was used in 15 cases and the median flap in three. This method proved to be successful for 17 patients. One patient who received a preoperative full dose of irradiation developed a fistula. There were two types of healing of the skin flap. The postoperative external appearance and function of the tongue were commendable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01886296
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  • 3
    Electronic Resource
    Electronic Resource
    An electron microscopic study of the muscle spindle in the arytenoid muscle of the human larynx (1987)
    Springer
    European archives of oto-rhino-laryngology and head & neck 244 (1987), S. 249-252 
    Details
    ISSN: 1434-4726
    Keywords: Muscle spindle ; Transmission electron microscope ; Human larynx ; Arytenoid muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We used transmission electron microscopy to examine a muscle spindle found in serial sections of the arytenoid muscle of the human larynx. Eight intrafusal fibers were observed at the equatorial region and contained two types of muscle fibers, nuclear bag fibers and nuclear chain fibers. The sensory nerve endings found on the nuclear bag fibers were annular or varicose in shape and contained numerous mitochondria. Another small sensory ending was composed of many vesicles and some dense granules. The fusimotor nerve endings found on the nuclear chain fibers had three types of endings with different sizes and complexities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455315
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  • 4
    Electronic Resource
    Electronic Resource
    Ciliated mucous cells found in the nasal mucosa of a patient with Kartagener's syndrome (1988)
    Springer
    European archives of oto-rhino-laryngology and head & neck 244 (1988), S. 360-362 
    Details
    ISSN: 1434-4726
    Keywords: Nasal mucosa ; Kartagener's syndrome ; Ciliated mucous cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Transmission electron microscopy revealed the presence of ciliated cells containing many mucous secretory granules in the nasal surface epithelium of a 13-year-old patient suffering from Kartagener's syndrome. In these cells, mucous secretory granules were accumulated in the apical cytoplasm, and the Golgi apparatus was well developed in the supranuclear region. Mucous secretory granules were discharged infrequently through the apical cell membranes by single or compound exocytosis. The cells were considered to be ciliated mucous cells, which have already been reported to be present in the lower respiratory tract but not in the upper respiratory tract.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00497465
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  • 5
    Electronic Resource
    Electronic Resource
    Immunocytochemical detection of triphosphoinositide in vestibular hair cells (1985)
    Springer
    Histochemistry and cell biology 82 (1985), S. 197-199 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Triphosphoinositide (TPI), an aminoglycoside receptor and a possible regulator of cationic permeation through its ability to bind with Ca++, was localized by the protein-A gold technique in vestibular sensory epithelia using an antibody highly specific to TPI. TPI was detected on the stereocilia, kinocilia, and cuticular plate of hair cells, and in the reticular membrane of supporting cells. The cilia of hair cells are damaged by aminoglycosides at a relatively early stage of toxicity. Ca++-regulated bioactivity in this area is probably involved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00708205
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  • 6
    Electronic Resource
    Electronic Resource
    Polymyxin-B-binding sites in the cochlea as demonstrated by polymyxin-B/gold labeling (1986)
    Springer
    Histochemistry and cell biology 86 (1986), S. 191-194 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A polymyxin-B/bovine-serum-albumin/gold complex was used as a probe to detect the binding sites of polymyxin B on thin sections of cochlea embedded in Spurr's resin. The binding sites were found to be mainly located on the stereocilia, the cuticular plate of hair cells, the head plate of Deiters' cells, the tonofilaments in pillar cells and Deiters' cells, fibrous structures in the spiral limbus, the tectorial membrane and the basilar membrane and neural elements such as nerve endings, fibers, and the myelin sheath. The mitochondria, plasma mimbrane, and chromatin of the nuclei of the cells observed also exhibited binding. Our results suggest that phospholipids, glycoconjugates, cytoskeletal proteins and nucleic acids are responsible for this binding activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493386
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  • 7
    Electronic Resource
    Electronic Resource
    Aminoglycoside binding sites in the cochlea as revealed by neomycin-gold labelling (1986)
    Springer
    Histochemistry and cell biology 85 (1986), S. 301-304 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neomycin/bovine serum albumin/gold was used as a probe to detect the binding sites of aminoglycosides on the thin sections of the cochlea embedded in Spurr. The binding sites were mainly located on the stereocilia, the cuticular plate of hair cells, the head plates of Deiters' cells, fibrous structures in pillar cells, in the spiral limbus and tectorial membrane and basilar membrane, plasma membranes, mitochondria and the chromatin of various kinds of cells. Triphosphoinositide, acidic glycosaminoglycans, and RNA were considered to be responsible for the binding activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493481
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  • 8
    Electronic Resource
    Electronic Resource
    Localization of triphosphoinositide in the cochlea (1984)
    Springer
    Histochemistry and cell biology 81 (1984), S. 157-160 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Triphosphoinositide (TPI) has been demonstrated to be a receptor for aminoglycosides in the cochlea and may regulate ionic permeability by its binding with Ca++. This phospholipid was localized by a protein A-gold technique in the cochlea at the electronmicroscopic level. TPI was prepared by a neomycin column and antibodies to it were raised in rabbits. The antibody used in this study reacted virtually only to TPI among the tested lipids. TPI was localized mainly at stereocilia, cuticular plates, head plates of Deiter's cells, plasma membrane, and mitochondria of various cells in the organ of Corti. In the vascular stria, TPI was found mainly at the plasma membrane of basal infoldings of the marginal cells. Possible physiological and pathophysiological roles of TPI in the cochlea are briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490110
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  • 9
    Electronic Resource
    Electronic Resource
    Binding sites of an aminoglycoside in the cochlea examined by immunocytochemistry (1985)
    Springer
    Histochemistry and cell biology 83 (1985), S. 237-240 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To localize the binding sites of aminoglycosides in the cochlea, immunocytochemistry was used with the antibody to gentamicin and the protein-A/gold complex. We found that the main binding sites were the stereocilia, the cuticular plates of hair cells, the head plates of Deiters' cells, cell filaments and the cones of pillar cells, tectorial membranes, basilar membranes, the matrix of the spiral limbus, plasma membranes, mitochondria, and the chromatin of various kinds of cells. Triphosphoinositide and acidic glycosaminoglycans are the two most likely candidates for the cause of binding activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00953990
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  • 10
    Electronic Resource
    Electronic Resource
    Serial section method for cytofluorometric determinations of the DNA content of component cells of the human cochlea (1987)
    Springer
    Histochemistry and cell biology 87 (1987), S. 115-121 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We describe a method for measuring the DNA content of the component cells of the organ of Corti using serial sections of human cochleae obtained at autopsy. Cochleae were fixed in Carnoy's solution and embedded in Acrytron E, a water-miscible methacrylate resin. A procedure was developed to reduce the background fluorescence in methacrylate-embedded sections; the resin was pretreated with ion-exchange resin (Amberlite IRA-410). Experiments showed that pretreatment reduce the background fluorescence practically to zero. Seventy 3 μm-thick serial sections were prepared on fluorescence free glass slides and stained with azocarmin G and acriflavine-Feulgen. After postirradiation using blue excitation light, the amount of Feulgen-DNA present in the target nucleus in each section was determined using a microfluorometer. The amount of DNA in the entire nucleus was determined by adding together the DNA contnet of the segments of the nucleus. The characteristic appearance of the organ of Corti made it easy to detect these cells; under green excitation light the cells of this organ exhibited red cytoplasmic azocarmin-G fluorescence. Due to the relatively wide internuclear spaces, cytofluorometry of individual nuclei could be performed without interference from the neighboring cells. Our technique using serial sections allowed us to measure the DNA contnet of individual cells and obtain histological information about particular cells and their neighboring cells. Several polyploid cells were found among the Hensen's cells in the cochlea, while all other component cells of the organ of Corti were diploid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00533395
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