ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
: Nucleotide sequence and polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) analysis of the ribosomal RNA gene (rDNA) regions containing the internal transcribed spacers (ITSs) and the 5.8S rRNA coding sequence was used to differentiate between 7 typical Flammulina strains. These nucleotide sequences revealed the presence of strain-specific deletions, insertions, and substitutions. Moreover, RFLP patterns produced using restriction endonucleases DraI, FokI, HaeII, MboII, and NlaIV, enabled identification of specific Flammulina strains. Thus, PCR-RFLP analysis of the ITS regions appears to be a useful tool for the identification of Flammulina strains.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2002.tb08763.x
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