ISSN:
1600-0714
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Proteolytic enzyme activity has been shown to be important for cyst formation. In this study, we constructed a cyst-like structure in vivo and analyzed molecular mechanisms involved in the development of the lesion. When SV40-immortalized duct cells of normal human salivary gland (NS-SV-DC) were treated with TGF-βl at a concentration of 1 ng/ml or 5 ng/ml followed by co-inoculation with Matrigel into the backs of nude mice, they formed large cysts containing fluid when 5 ng/ml of TGF-βl was used. Analysis of the fluid demonstrated high MMP activity. Immunohistochemical staining exhibited strong reactivity with anti-MMP-2 antibody in TGF-pl (5 ng/ml)-treated NS-SV-DC. Northern blot analysis indicated that the expression of TGF-β1 and MMP-2 mRNAs in ceils was greatly enhanced by treatment with 5ng/ml TGF-βl. These findings suggest that the in vivo cyst formation by TGF-βl-treated cells is associated with continuous induction of MMP-2 activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0714.1996.tb00299.x
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