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  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical study of cytochrome P450 2C and 3A in human non-neoplastic and neoplastic tissues (1999)
    Springer
    Virchows Archiv 434 (1999), S. 401-411 
    Details
    ISSN: 1432-2307
    Keywords: Key words Human ; Cytochrome P450 2C ; Cytochrome P450 3A ; Immunohistochemistry ; Neoplasms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Organ and cellular distribution and expression constancy of microsomal cytochrome P450 (CYP) 2C and 3A in humans were studied with new polyclonal antibodies to CYP2C (MP-1) and 3A (NF-2) active in formalin-fixed, paraffin-embedded tissues. Antibodies were raised against purified human CYP2C9 and CYP3A4. On western blotting, MP-1 reacted with 2C8, 2C9, 2C18 and 2C19, and NF-2 with 3A4. In both frozen and paraffin sections, hepatocytes showed diffuse immunoreactivity with MP-1 and centrilobular staining with NF-2. Inparaffin sections of 40 kinds of nonneoplastic tissues, epithelium of the small and large intestine, bile duct, nasal mucosa, kidney and adrenal cortex stained positively with both MP-1 and NF-2 antibodies. Epithelium of gastric fundic glands, salivary glands, tracheobronchial glands, Brunner’s glands, the prostate, uterine cervix and nasopharynx showed definite reactivity with MP-1. Epithelium of the gastric mucosa with intestinal metaplasia, duodenum, gallbladder and intercalated ducts of the pancreas and chief cells of the parathyroid and the corpus luteum of the ovary reacted with NF-2. Among the neoplastic tissues, MP-1 reacted with pleomorphic adenoma of the salivary gland and carcinomas of six different organs, and NF-2 with those of 7 different organs. These results indicate that CYP2C and CYP3A are distributed widely and organ specifically, as well as being variably expressed in neoplastic and normal states.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280050359
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  • 2
    Electronic Resource
    Electronic Resource
    Hamartoma of the parotid gland: A case report with immunohistochemical and electron microscopic study (1987)
    Springer
    Virchows Archiv 411 (1987), S. 473-478 
    Details
    ISSN: 1432-2307
    Keywords: Hamartoma ; Parotid gland ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of a solid parotid tumour in a 16-year-old boy is presented. Histologically, the tumour demonstrated some peculiar findings. An acinar pattern was predominant although every component seen in the normal salivary gland was present, namely, serous and mucous gland acini, ducts, myoepithelial cells, adipose and lymphoid tissue. Large eosinophilic granules were abundant in the large acinar cell cytoplasm. Immunohistochemically, the tumour demonstrated the proteins which are present in the normal parotid gland, for example, amylase, lactoferrin and lysozyme. Electron microscopic features were quite similar to those of normal parotid tissue except for accumulation of a large number of cytoplasmic granules in the acinar cells. There has been no previous report of a tumour with the same features as seen in this case. Our pathological diagnosis is hamartoma, although the possibility of hyperplasia or neoplasia can not be excluded.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00735229
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  • 3
    Electronic Resource
    Electronic Resource
    Cholangiocarcinoma arising in bile duct adenoma with focal area of bile duct hamartoma (1995)
    Springer
    Virchows Archiv 426 (1995), S. 209-213 
    Details
    ISSN: 1432-2307
    Keywords: Bile duct adenoma ; Cholangiocarcinoma ; Bile duct hamartoma ; Proliferating cell nuclear antigen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 59-year-old male with history of sigmoid colon cancer had a high serum-CEA level and was referred for the evaluation of metastatic liver disease. Ultrasonography and computerized tomography showed two tumours in the liver. Macroscopically, these were in segment 4 (S4) and 2 (S2). Histologically, the tumour in S4 showed a number of bile ductules with variable amounts of stroma, an appearance compatible with bile duct adenoma (BDA). There were markedly atypical ductules of various sizes, the epithelium of which had coarsely granular/hyperchromatic large nuclei, in some areas of the lesion. These atypical ductules showed invasive growth into the liver parenchyma. Some cystically dilated ductules with bile plugs resembling bile duct hamartoma (BDH) were also seen. The other tumour in S2, was a metastatic adenocarcinoma from sigmoid colon and showed strongly positive staining for CEA. Since the lesion in S4 of our case is solitary and most of histological features are similar to those of BDA with markedly atypical bile ductules, we consider that this may be the first case of cholangiocarcinoma associated with BDA with focal area of BDH. It is possible that the adenoma-carcinoma sequence occurs in biliary tumours.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192644
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  • 4
    Electronic Resource
    Electronic Resource
    Fibrotic focus in infiltrating ductal carcinoma of the breast: A significant histopathological prognostic parameter for predicting the long-term survival of the patients (1998)
    Springer
    Breast cancer research and treatment 49 (1998), S. 195-208 
    Details
    ISSN: 1573-7217
    Keywords: infiltrating ductal carcinoma ; breast cancer ; histology ; fibrotic focus ; prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The presence of fibratic fows (FF) in infiltrating ductal carcinoma (IDC) has been shown to be an important histological factor associated with high tumor aggressiveness, or early tumor recurrence or death. However, the clinicopathological significance of FF for predicting the long-term survival of the patients with IDC has not been fully investigated. In order to elucidate this aspect, we divided 140 IDCs with at least 10 years of follow up into tumors with FF and those without. IDC with FF showed significantly higher histologic grade (P=0.02), higher frequency of tumor necrosis (P=0.02), higher frequency of cases with more than three positive lymph node metastases (P=0.04), higher T classification (P=0.009), and higher pathological stage (P=0.0002) than those without FF. Relative risk (RR) of tumor recurrence and death was significantly higher in tumors with FF than in those without (RR=4.5, P 〈 0.00001 and RR=5.6, P 〈 0.00001, respectively). In cases of early stage cancer (stages I, IIA, and IIB), or in those with less than four lymph node metastases, IDCs with FF demonstrated a significantly higher risk than those without. Multivariate adjustments for other pathological factors did not change the RRs significantly. These results indicate that in long-term follow up the presence of FF is a significant prognostic parameter for IDC, and therefore strongly suggest that IDCs must be divided into those with and without FF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006067513634
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  • 5
    Electronic Resource
    Electronic Resource
    Peripheral T-cell lymphoma (1988)
    Springer
    Cancer and metastasis reviews 7 (1988), S. 243-261 
    Details
    ISSN: 1573-7233
    Keywords: lymphoma/leukemia ; T-lymphocytes ; pathology ; adult T-cell leukemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Since the discovery of human T-cell leukemia virus type 1 (HTLV-1) in patients with adult T-cell leukemia/lymphoma (ATLL), malignant neoplasms of mature (peripheral) T lymphocytes have attracted a great deal of attention. This type of neoplasm is more common in Japan than in Western countries, and may show distinct clinical pictures such as hypergammaglobulinemia, hypercalcemia, etc. T-cell lymphomas are more prone than B-cell lymphomas to become leukemic. Because of a marked intermingling of reactive cells (histiocytes, eosinophils, etc.), the histologic diagnosis of T-cell lymphoma is often difficult. Proliferation pattern and cellular size do not correlate with prognosis as in B-cell lymphoma. Since T-cell lymphomas often manifest with several distinct clinicopathologic settings, their categorization should be based on several parameters, such as the presence or absence of ATLL-associated antigen in serum, histology, phenotype of the neoplastic cell, and clinical features. Since a classification for T-cell lymphomas has not been established, a further multi-disciplinary approach is necessary for a better understanding of this interesting neoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00047754
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  • 6
    Electronic Resource
    Electronic Resource
    Prevention of hemoglobinuria by administration of haptoglobin (1980)
    Springer
    Research in experimental medicine 177 (1980), S. 1-12 
    Details
    ISSN: 1433-8580
    Keywords: Hemolysate ; Hemoglobinuria ; Haptoglobin ; Iron deposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This report describes the role of haptoglobin in preventing hemoglobinuria. In rabbits, human haptoglobin administered i.v. worked preventively against the development of hemoglobinuria in spite of the presence of a large amount of hemolysate containing free hemoglobin. More specifically: (1) the kidney did not present a dark brown color, (2) the deposition of iron in the kidney was significantly less than that in the same organ of rabbits given hemolysate only, and (3) the microangiographic and histological findings of the kidney were close to normal patterns. From the above it may be concluded that the administration of haptoglobin is effective for the prevention of hemoglobinuria and subsequent renal damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851626
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  • 7
    Electronic Resource
    Electronic Resource
    Nuclear accumulation of beta-catenin in intestinal-type gastric carcinoma: correlation with early tumor invasion (2000)
    Springer
    Virchows Archiv 437 (2000), S. 508-513 
    Details
    ISSN: 1432-2307
    Keywords: Gastric carcinoma Beta-catenin Nuclear accumulation Mutational analysis Invasive front
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Mutation of the adenomatous polyposis coli gene, which is known to be an early event in the carcinogenesis of intestinal-type gastric carcinoma, leads to accumulation of beta-catenin. In addition, beta-catenin has been found to activate down stream signaling molecules in the wingless/Wnt pathway. In this study, the clinical significance of nuclear accumulation of beta-catenin was evaluated in gastric carcinoma. Immunohistochemical staining showed nuclear localization in 16 (12%) of 139 (94 intestinal-type and 45 diffuse-type) gastric carcinomas, and all 16 lesions with nuclear staining were intestinal-type adenocarcinomas. Of the 16 cases, 15 were in the early clinical stage. In the remaining case, the lesion had invaded the subserosal layer and showed strong nuclear staining at the invasive front. In 14 of the 16 cases with nuclear localization, there were no abnormal mobility shifts detected using polymerase chain reaction-single strand conformational polymorphism analysis. This was confirmed using direct sequencing analysis, which revealed the wild-type sequence in the 12 cases tested. Nuclear accumulation of beta-catenin did not correlate with lymph node metastasis or 5-year survival. These findings suggest that high intranuclear levels of beta-catenin protein play an important role in early tumor growth and may function in initiation of invasive processes in intestinal-type gastric carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280000283
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  • 8
    Electronic Resource
    Electronic Resource
    Pituitary adenomas in patients under 20 years old. A clinicopathological study of 12 cases (1986)
    Springer
    Journal of neuro-oncology 4 (1986), S. 79-89 
    Details
    ISSN: 1573-7373
    Keywords: Pituitary adenoma ; prolactinoma ; Cushing's syndrome ; hypopituitarism ; hypogonadism ; immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The clinical manifestations, pathological features and follow-up data on 12 cases of pituitary adenoma in patient less than 20 years old were evaluated. This group represented 8.5% of our 142 cases of pituitary adenoma from all age groups during the period of study. There were five males and seven females whose ages ranged from 13 to 19 years at diagnosis. Immunocytochemical staining demonstrated the presence of prolactin in 10 tumors, ACTH in one tumor and the remaining neoplasm was negative for the five major pituitary hormones (prolactin, hGH, ACTH, TSH, gonadotrophin). The results of the immunocytochemistry correlated appropriately with the clinical manifestations. Extension beyond the sella turcica at presentation was a common feature as evidenced by the high incidence of visual defects (75%). A complete excision was accomplished in only two patients. The aggressive behavior of these tumors was demonstrated by a recurrence rate of 50% and only a single long-term cure. Early detection and therapy, if possible, are essential for the successful management of pituitary adenomas in younger patients as implied by our study and other reports in the literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02158007
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  • 9
    Electronic Resource
    Electronic Resource
    Studies of hCG binding and endocytosis in rat ovary by ultrastructural immunocytochemistry (1982)
    Springer
    Cell & tissue research 224 (1982), S. 647-653 
    Details
    ISSN: 1432-0878
    Keywords: hCG binding sites ; Endocytosis ; Ovary ; Ultrastructural immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Localization of hCG binding sites and the process of endocytosis in pseudopregnant rat ovaries were investigated by indirect electron-microscopic immunocytochemistry. Immature female rats were treated with pregnant-mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) to induce ovarian luteinization. Eight days after priming with PMSG-hCG and 1–6 h before sacrifice the animals were given another injection of hCG to bind the receptors. Receptor sites to hCG localized by reaction product were present in most luteal cells, but not in primary follicular cells. The receptor sites were distributed on luteal cell surfaces facing interstitial spaces. Endocytotic pits containing hCG binding sites were rarely seen 1 h after hCG injection. At 2 h, hCG and presumably its receptor were taken up within endocytotic vesicles with the evidence of reaction product coated on the vesicle wall. With time, fusion of endocytotic vesicles with lysosome occurred and the reaction product appeared in phagolysosomes. The reaction product was localized on phagolysosomal inner surface or in free granular form. These findings suggest that hCG and its receptors were internalized through endocytotic pits and endocytotic vesicles and delivered to lysosomes probably for degradation. An additional experiment for localization of acid phosphatase was also performed to delineate the lysosomes and phagolysosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213759
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