ISSN:
1432-0533
Keywords:
EAE Serum
;
Myelin Inhibition
;
Tissue Culture
;
Oligodendroglia
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Myelinating mouse spinal cord fragments were cultured in Maximow assemblies. A variable interval after explanation they were exposed to low concentrations of serum from rabbits with experimental allergic encephalomyelitis (EAE). As a general rule, the longer they were kept under EAE serum, the more completely myelination was inhibited. If the experiments were made with low concentrations of EAE serum (about 1%), the number of myelinated cultures was proportional to the number of days by which the application of EAE-serum was delayed. If one worked with EAE-serum at higher concentrations (5%), the cultures on which the serum was applied with a delay from 1 to 5 days were practically completely inhibited in their myelination. If exposed from the 7th day onward only, nearly all of these cultures were able to myelinate and therefore showed myelin, when viewed on day 12 in vitro. If, however, they were left under the same concentrations of EAE-serum for another week, the myelin sheaths disappeared again without leaving any signs of myelin destruction. These cultures showed loose spirals of glial cytoplasma membranes around axons. Cultures in which the application of EAE-serum was delayed for 4–6 days showed differentiated oligodendrocytes and astrocytes, which was not the case in cultures inhibited from the explantation onward.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00687558
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