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  • 1
    Digitale Medien
    Digitale Medien
    QTL analysis of Cercospora leaf spot resistance in sugar beet (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: The inheritance of Cercospora leaf spot resistance in sugar beet was investigated by means of quantitative trait loci (QTL) analysis of a segregating population of 193 individuals, using 110 AFLP and 35 restriction fragment length polymorphism (RFLP) markers. Five QTL were found through composite interval mapping on linkage groups 1, 2, 3, and 9, respectively, two of which were linked on linkage group 3. The significance of these QTL was tested by permutation analysis The QTL had mostly additive, but also certain negative dominance effects; all the resistance alleles came from the Cercospora-resistant parent. Each quantitative trait locus accounted for 7-18% of the phenotypic variation, leaving 37% of the variation unexplained. The results are discussed in relation to the potential use of marker-assisted breeding for Cercospora leaf spot resistance in sugar beet.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1439-0523.1999.00390.x
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  • 2
    Digitale Medien
    Digitale Medien
    The use of bulked segregant analysis to accumulate RAPD markers near a locus for beet cyst nematode resistance in Beta vulgaris (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 116 (1997), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Bulked segregant analysis (BSA) was used to accumulate RAPD markers near the beet cyst nematode resistance locus Hslpro-1 of sugar beet (Beta vulgaris L.). Graphical genotypes constructed from RFLP data were utilized to select F2 individuals in (1) the construction of pools of plants used in the initial screening for polymorphisms, and (2) the selection of individual plants used to confirm the potential linkage. The pooled DNA samples were screened for polymorphisms using 668 RAPD primers. Forty-four candidate markers potentially linked to the region were analysed further using 14 segregating individuals. Close linkage was confirmed for 17 of the markers. Four of the RAPD markers were assigned map coordinates within the RFLP map. Three of these markers extended the RFLP map by 3cM. Altogether, the 8cM target interval contains 10 RFLP and 17 RAPD markers, corresponding to an average marker density of 0.3cM in the Hslpro-1 region.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.1997.tb00970.x
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  • 3
    Digitale Medien
    Digitale Medien
    Chiasma and recombination data in plants: are they compatible?
    Amsterdam : Elsevier
    Trends in Genetics 9 (1993), S. 344-348 
    Details
    ISSN: 0168-9525
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9525(93)90038-J
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  • 4
    Digitale Medien
    Digitale Medien
    Reply
    Amsterdam : Elsevier
    Trends in Genetics 10 (1994), S. 114-115 
    Details
    ISSN: 0168-9525
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9525(94)90205-4
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  • 5
    Digitale Medien
    Digitale Medien
    Linkage disequilibrium and fingerprinting in sugar beet (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 323-326 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words AFLP ; Fingerprinting ; Linkage disequilibrium ; Sugar beet
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  It has been suggested that map information for molecular markers can be used to strengthen finterprinting analyses. The success of this strategy depends on the distribution of linkage disequilibrium over the genome. Using 451 mapped AFLP markers, we investigated the occurrence of linkage disequilibrium in nine sugar beet breeding lines. A low but significant level of linkage disequilibrium was found for unlinked markers. Only for very tigthly linked (〈3 cM) markers was this level substantially higher. This implies that little is gained in utilising the map position of the markers in fingerprinting applications.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051486
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  • 6
    Digitale Medien
    Digitale Medien
    Evaluation of RFLP and RAPD markers in a comparison of Brassica napus breeding lines (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 123-128 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Brassica napus ; RFLP markers ; RAPD markers ; Genetic distance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract RFLP and RAPD markers were evaluated and compared for their ability to determine genetic relationships in a set of three B. napus breeding lines. Using a total of 50 RFLP and 92 RAPD markers, the relatedness between the lines was determined. In total, the RFLP and the RAPD analysis revealed more than 500 and 400 bands, respectively. The relative frequencies of loci with allele differences were estimated from the band data. The RFLP and RAPD marker sets detected very similar relationships among the three lines, consistent with known pedigree data. Bootstrap analyses showed that the use of approximately 30 probes or primers would have been sufficient to achieve these relationships. This indicates that RAPD markers have the same resolving power as RFLP markers when used on exactly the same set of B. napus genotypes. Since RAPD markers are easier and quicker to use, these markers may be preferred in applications where the relationships between closely-related breeding lines are of interest. The use of RAPD markers in fingerprinting applications may, however, not be warranted, and this is discussed in relation to the reliability of RAPD markers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222404
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  • 7
    Digitale Medien
    Digitale Medien
    Crossover distribution in barley analysed through RFLP linkage data (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 211-216 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Hordeum vulgare ; RFLP ; Genetic map ; Recombination ; Crossover
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The pattern of recombination in barley with regard to (1) the distribution of crossover points among whole gametes, (2) the distribution of crossover points among individual chromosomes and (3) the distribution of crossover points within chromosomes has been analysed using data sets underlying two recently published restriction fragment length polymorphism (RFLP) linkage maps representing male and female meiosis, respectively. The data indicated that the process of recombination had been random with no interference. The two data sets gave similar results, indicating that male and female meiosis in barley do no differ significantly. The possibility of using RFLP data in studies of crossover distribution is stressed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00225144
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  • 8
    Digitale Medien
    Digitale Medien
    Evaluation of AFLP in Beta (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 845-852 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words AFLP ; Beta ; Polymorphism ; Reproducibility ; Sugarbeet
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  AFLP markers were evaluated for their usefulness in the genetic analysis of sugarbeet and wild Beta species. Accessions of ten different sugarbeet breeding lines and five wild beets were screened using 256 primer combinations. Of the 11 309 bands investigated, 96.4% were polymorphic among the accessions. A strong positive correlation was found between the number of polymorphisms and AT content of the selective bases of the primer combinations. Random subsets of primer combinations were used to produce genetic distance trees. Permutation tests showed that, for the wild beets, 500 AFLP bands sufficed to obtain the best topology of the tree with a probability at any given node of more than 99%. Ten times as many bands were necessary to obtain support values of the same order of magnitude for the sugarbeet lines. The reproducibility of AFLP for seven primer combinations was investigated by repeated analysis of all steps from DNA isolation to data scoring. For 5088 comparisons, the overall reproducibility was 97.6%. Robustness to genotyping errors was investigated by including an artificial F1 (1 : 1 DNA mixture) of two sugarbeet lines in the screen for polymorphisms. For the 3160 cases of polymorphism between the two lines, 0.2% genotyping errors were found. The general reliability and usefulness of AFLP markers are discussed in relation to the results obtained.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220051143
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  • 9
    Digitale Medien
    Digitale Medien
    Competition as a source of errors in RAPD analysis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1185-1192 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Random amplified polymorphic DNA ; Competition ; DNA mixtures
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have used artificial 1∶1 DNA mixtures of all pairwise combinations of four doubled haploid Brassica napus lines to test the ability of RAPDs to function as reliable dominant genetic markers. In situations where a specific RAPD band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in the 1∶1 DNA mixture. In 84 of all 613 heterozygous situations analysed, the expected band failed to amplify in the RAPD reaction. Thus, RAPD markers will lead to an erroneous genetic interpretation in 14% of all cases. In contrast, the formation of non-parental heteroduplex bands was found at a frequency of only 0.2%. Analysis of 1∶ 1 mixtures using (1) a different set of optimized reaction conditions and (2) a material with low genomic complexity (Bacillus cereus) gave identical results. Serial dilutions of one genome into another, in steps of 10%, showed that all of the polymorphic bands decreased in intensity as a linear function of their respective proportion in the mixture. In dilutions with water no differences in band intensity were detected. Thus, competition occurs in the amplification of all RAPD fragments and is a major source of genotyping errors in RAPD analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00223449
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  • 10
    Digitale Medien
    Digitale Medien
    Competition as a source of errors in RAPD analysis (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1185-1192 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Key words Random amplified polymorphic DNA  ;  Competition  ;  DNA mixtures
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have used artificial 1:1 DNA mixtures of all pairwise combinations of four doubled haploid Brassica napus lines to test the ability of RAPDs to function as reliable dominant genetic markers. In situations where a specific RAPD band is present in one homozygous line but absent in the other, the band is expected in the artificial heterozygote, i.e. in the 1:1 DNA mixture. In 84 of all 613 heterozygous situations analysed, the expected band failed to amplify in the RAPD reaction. Thus, RAPD markers will lead to an erroneous genetic interpretation in 14% of all cases. In contrast, the formation of non-parental heteroduplex bands was found at a frequency of only 0.2%. Analysis of 1: 1 mixtures using (1) a different set of optimized reaction conditions and (2) a material with low genomic complexity (Bacillus cereus) gave identical results. Serial dilutions of one genome into another, in steps of 10%, showed that all of the polymorphic bands decreased in intensity as a linear function of their respective proportion in the mixture. In dilutions with water no differences in band intensity were detected. Thus, competition occurs in the amplification of all RAPD fragments and is a major source of genotyping errors in RAPD analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001220050355
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