ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
H6 is an Atriplex nummularia gene having high sequence homology with the algal caltractin (a basal-body-associated calcium-binding protein) gene. Recombinant H6 was expressed at high levels in Escherichia coli. The recombinant protein exhibited an ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid-induced mobility shift during sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was able to bind 45Ca2-. An H6 cDNA probe detected three RNA transcripts of 1.3, 2.2 and 2.4 kb, respectively. The levels of these transcripts were regulated by different environmental cues and during developmental stages. The steady state levels of the 1.3-kb mRNA decreased after touch and heat treatments. Expression of the 2.2-kb message correlated with cell proliferation activity. In cultured cells, the highest level of the 2.2-kb message preceeded the peak of mitotic cell division activity. In plants, the 2.2-kb message was detected only in shoot tips that contained meristematic tissues. The 2.4-kb message was detected exclusively in heat-shocked cells. The relationship among the three transcripts is discussed in the context of the possible role of H6 in mediating developmental and environmental signals.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1996.tb00509.x
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