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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of PCE-Dechlorinating Activity by Electrochemical Cultivation (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 20-21 (July 2007), p. 319-322 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Tetrachloroethene (PCE) is one of the grave environmental pollutants. Certain anaerobicbacteria have the ability to dechlorinate PCE as an electron acceptor for their respiration(dehalorespiration). Previously, we accumulated PCE-dechlorinating culture in the presence ofFe(III)-EDTA. In this study, we have described the regulation of dehalorespiration by electrolyticcontrol of the Fe(II)/Fe(III) ratio using a potentiostat (electrochemical cultivation).PCE-dechlorinating culture was cultivated under H2 conditions at constant redox potentials rangingfrom –0.1 to –0.8 V. After electrochemical cultivation for 3 weeks, PCE-dechlorinating activity ofthe mixed culture varied at different redox potentials. At –0.6 V, the culture showed the highestactivity and the highest ratio of Desulfitobacterium/total bacteria. Electrochemical cultivation is auseful method for regulating PCE-dechlorinating bacterium
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.319.pdf
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  • 2
    Electronic Resource
    Electronic Resource
    Enrichment of Chromium-Reducing Bacteria Using Potential-Controlled Electrochemical Cultivation (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Advanced materials research Vol. 20-21 (July 2007), p. 505-508 
    Details
    ISSN: 1662-8985
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We present here a method for the electrochemical enrichment of chromium-reducingbacteria from environmental samples containing various microorganisms. An electrochemicalcultivation system was constructed; this consisted of a Pt anode and a carbon cathode separated byan ion-exchange membrane. For an electrochemical cultivation, an environmental sample withchromium-reducing bacteria was inoculated into the anode well where a medium containing 0.1mM Cr(VI) ions was poured. On the other hand, Cr(VI)-free medium was poured into the cathodewell. Chromium-reducing bacteria grew selectively after 10 days of incubation and application of1.0 V (vs. Ag/AgCl) of anode potential that reproduces Cr(VI) ions. This result suggested thatCr(VI) ions generated on the anode promoted the growth of chromium-reducing bacteria as theirelectron acceptor and due to Cr(VI) toxicity, inhibited the growth of other microorganisms
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.505.pdf
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  • 3
    Electronic Resource
    Electronic Resource
    Symbiotic association in Chlorella culture (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 51 (2005), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Chlorella sorokiniana IAM C-212 has long been maintained in slant culture as a mixed strain, representing an associated natural microbial consortium. In this study, the consortium was separated and five nonalgal constituents, a fungal strain (CSSF-1), and four bacterial strains (CSSB-1, CSSB-2, CSSB-3, and CSSB-4) were isolated and identified. 16S rDNA sequence analysis revealed that strains CSSB-1, CSSB-2, CSSB-3, and CSSB-4 were close to Ralstonia pickettii (99.8% identity), Sphingomonas sp. DD38 (99.4% identity), Microbacterium trichotecenolyticum (98.6% identity), and Micrococcus luteus (98.6% identity) respectively. 18S rDNA sequence analysis revealed that strain CSSF-1 resembled Acremonium-like hyphomycete KR21–2 (98.8%). The fungal strain CSSF-1 and one of the bacterial strains, CSSB-3, were found to promote the growth of Chlorella while the presence of bacterial strains CSSB-1 and CSSB-2 had no effect. Strain CSSB-4 could not be subcultured so its role was not elucidated. These results show that the interaction between Chlorella and its symbionts under photoautotrophic conditions involved both mutualism and commensalisms. The chlorophyll content of mixed strain was stable in long-term cultivation (7 months) while the chlorophyll content of a pure culture showed a marked decline. Electron microscopic analysis showed the two bacterial strains CSSB-2 and CSSB-3 were harbored on the sheath excreted by Chlorella, while the fungal strain CSSF-1 and the bacterial strain CSSB-1 directly adhered to the Chlorella cell surface.This report is the first observation of a symbiotic relationship among fungus, bacteria, and Chlorella, and the first observation of direct adhesion of fungus and bacteria to Chlorella in a consortium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2004.08.004
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  • 4
    Electronic Resource
    Electronic Resource
    Desulfurization of coal by microbial column flotation (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 44 (1994), S. 125-131 
    Details
    ISSN: 0006-3592
    Keywords: Thiobacillus ferrooxidans ; flotation ; coal pyrite ; desulfurization ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Twenty-three strains capable of oxidizing iron were isolated from coal and ore storage sites as well as coal and ore mines, volcanic areas, and hot spring. Four strains were found to have high iron-oxidizing activity. One strain (T-4) was selected for this experiment since the strain showed the fastest leaching rate of iron and sulfate from pyrite among the four strains. The T-4 strain was assigned for Thiobacillus ferrooxidans from its cultural and morphological characteristics.Bacterial treatment was applied to column flotation. An increase of cell density in the microbial column flotation resulted in the increase of pyrite removal from a coal-pyrite mixture (high sulfur imitated coal) with corresponding decrease of coal recovery. The addition of kerosene into the microbial column flotation increased the recovery of the imitated coal from 55% (without kerosene) to 81% (with 50 μL/L kerosene) with the reduction of pyrite sulfur content from 11% (feed coal) to 3.9% (product coal). The kerosene addition could reduce the pyritic sulfur content by collecting the coal in the recovery. However, the addition could not enhance separation of pyrite from the coal-pyrite mixture, since pyrite rejection was not affected by the increase of the kerosene addition. An excellent separation was obtained by the microbial flotation using a long column which had a length-diameter (L/D) ratio of 12.7. The long column flotation reduced the pyritic sulfur content from 11% (feed coal) to 1.8% (product coal) when 80% of the feed coal was recovered without the kerosene addition. The long column flotation not only attained an excellent separation but also reduced the amount of cells for desulfurization to as little as one-tenth of the reported amount. © 1994 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260440118
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  • 5
    Electronic Resource
    Electronic Resource
    Mechanism of microbial flotation using Thiobacillus ferrooxidans for pyrite suppression (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 41 (1993), S. 671-676 
    Details
    ISSN: 0006-3592
    Keywords: Thiobacillus ferrooxidans ; bacterial adhesion ; flotation ; coal ; pyrite ; desulfurization ; ore dressing ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microbial desulfurization might be developed as a new process for the removal of pyrite sulfur from coal sluries such as coal-water mixture (CWM). An application of iron-oxidizing bacterium Thiobacillus ferrooxidans to flotation would shorten the periods of the microbial removal of pyrite from some weeks by leaching methods to a few minutes. The floatability of pyrite in flotation was mainly reduced by T. ferrooxidans itself rather than by other microbial substances in bacterial culture as additive of flotation liquor. Floatability was suppressed within a few seconds by bacterial contact. The suppression was proportional to increasing the number of cells observed between bacterial adhesion and the suppression of floatability. If 25% of the total pyrite surface area covered with the bacteria, pyrite floatability would be completely depressed. Bacteria that lost their iron-oxidizing activities by sodium cyanide treatment were also able to adhere to pyrite and reduced pyrite floatability as much as normal bacteria did. Thiobacillus ferrooxidans ATCC 23270, T-1, 9, and 11, which had different iron-oxidizing abilities, suppressed floatability to similar-levels. The oxidizing ability of bacteria did not influence the suppressing effect. These results showed the mechanism of the suppression of pyrite floatability by bacteria. Quick bacterial adhesion to pyrite induced floatability suppression by changing the surface property from hydrophobic. The quick adhesion of the bacterium was the novel function which worked to change the surface property of pyrite to remove it from coal. © 1993 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260410611
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