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  • 1
    Electronic Resource
    Electronic Resource
    PREPARATION OF AN IMITATION SOY SAUCE FROM HYDROLYZED DRIED YEAST CANDIDA UTILIS (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food processing and preservation 22 (1998), S. 0 
    Details
    ISSN: 1745-4549
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Acid hydrolysis of dried fodder's yeast Candida utilis was studied by a central composite design taking into account three independent variables: temperature (105–165C), processing time (30–70 min) and HCl concentration (7.5–17.5%). Response variables were: color, dry matter solubilization and amino nitrogen. Temperature was the most significant variable followed by acid concentration and processing time. Response surfaces were generated from fitted polynomials. Higher temperatures to prevent amino nitrogen degradation were not investigated. As different effects were found for color development and amino nitrogen, a combined function was tested which yields the behavior of all responses simultaneously. The best conditions were: 150C, 15% HCl and 40 min. After neutralization with 6N NaOH and centrifugation, the raw sauce was supplemented with 12% NaCl, 0.1% monosodium glutamate, 2% vinegar and 0.1% sodium benzoate and evaluated by sensory analysis for eight distinct attributes. Imitation sauce from yeast was at least as good as commercial hydrolyzed soybean sauce.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4549.1998.tb00360.x
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  • 2
    Electronic Resource
    Electronic Resource
    Enzyme activities and substrate degradation during white rot fungi growth on sugar-cane straw in a solid state fermentation (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 210-212 
    Details
    ISSN: 1573-0972
    Keywords: Enzyme activities ; lignocellulosics ; mushroom ; Pleurotus spp. ; sugar-cane straw ; white rot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Two strains of Pleurotus spp., grown in solid state fermentation on sugar-cane straw, degraded the dry matter by 50% after 60 days. The rate of substrate consumption and the dry weight of fruiting bodies decreased in consecutive flushings. Both strains vigorously attacked hemicellulose (80% of total degradation) and lignin (70%). Fruiting bodies were rich in protein and lipids, and had a low content of carbohydrates and ash.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327839
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  • 3
    Electronic Resource
    Electronic Resource
    Bioconversion of sugar cane crop residues with white-rot fungiPleurotus sp. (1992)
    Springer
    World journal of microbiology and biotechnology 8 (1992), S. 402-405 
    Details
    ISSN: 1573-0972
    Keywords: Amino acid ; cellulose ; lignin ; nitrogen content ; Pleurotus ; solid state fermentation ; sugar cane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Four mushroom strains ofPleurotus spp. were cultivated on sugar cane crop residues for 30 days at 26°C. Biochemical changes affected the substrate as a result of fungal growth, in terms of nitrogen, lignin, cellulose and hemicellulose contents. All strains showed a strong ligninolytic activity together with variable cellulolytic and xylanolytic action.Pleurotus sajor-caju attacked lignin and cellulose at the same rate, showing a degradation of 47% and 55%, respectively. A better balance was shown by theP. ostreatus-P. pulmonarius hybrid, which exhibited the poorest cellulolytic action (39%) and the highest ligninolytic activity (67%). The average composition of mushroom fruit bodies, in terms of nitrogen, carbohydrates, fats and amino acid profiles, was determined. Crude protein and total carbohydrate varied from 23% to 33% and 36% to 68% of dry matter, respectively. Fat ranged from 3.3% to 4.7% and amino acid content from 12.2% to 22.2%. Slight evidence for a nitrogen fixing capability was encountered in the substrate to fruit body balance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01198754
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  • 4
    Electronic Resource
    Electronic Resource
    Cross flow filtration of RNA extracts by hollow fiber membrane (1992)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 12 (1992), S. 49-56 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The feasibility of using ultrafiltration to concentrate RNA was investigated. Ultrafiltration flux and solute retentivity were affected by membrane cut-off and inlet pressure.The RNA can be concentrated by selecting a hydraulically permeable membrane (PM 30) and then operating the system at a sufficiently high pressure (2.07 · 105 Nm-2) and a high recirculation rate (150 cm-1). The use of this procedure was limited to a high-flux regime. However, a very high retentivity of macrosolute was achieved. The effect of transmembrane pressure on the apparent mass transfer coefficient in the “gel polarization model” was discussed and related to ultrafiltration flux.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370120113
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  • 5
    Electronic Resource
    Electronic Resource
    Behaviour of Candida utilis during growth in relation to Mg2+ and K+ concentration in sugar cane molasses (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 111-114 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Magnesium and potassium ions are present in sugar cane molasses in a concentration of about 0.3% and 2.5%, respectively, which is high enough to support biomass production from Candida utilis. Culture broth with 40 g/l of total reducing substances supplemented by the addition of 1 ppm of Mg2+ leads to a higher yield (Y x/s) in batch fermentation experiments. The subsequent addition of Mg2+ up to 10 ppm decreases the yield coefficient from 0.53 to 0.42 without affecting the growth rate. Fermentation media supplemented with 1 to 10 ppm of K+ decreased both the yield coefficient and the specific growth rate. A Mg/K ratio of about 0.1 seems to be optimal for yeast biomass propagation.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140118
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  • 6
    Electronic Resource
    Electronic Resource
    RNA removal from Candida utilis by chemical and thermal treatments (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 145-153 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The removal of the content of nucleic acids of fodder yeast (Candida utilis) by treatment with HCl or heat shock was investigatedAcid concentrations between 5 and 15% (on dried matter basis) were used. A maximal removal of the content of nucleic acids of 88% was realized wheńn was worked with 15% HCl, 90 °C during 30 minutes. But under this conditions were observed high demanges of protein and also of some essential amino acidsGood results for diminishing the content of nucleic acids were reached with the highest concentration of acid and a treatment during 20 minutesThe experiments with heat shock were carried out at 68 °C, different times for heating and different contents of yeastIn this way better results than for treatment with acid according to diminishing the content of nucleic acids and yield of protein and essential amino acids were reachedA removal of over 80% of the content of nucleic acids was achieved in all cases.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020205
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  • 7
    Electronic Resource
    Electronic Resource
    The influence of MAILLARD's reaction in sugar cane molasses on the kinetic fermentation parameters of Candida utilis NRRL Y-660 (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 53-59 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growth inhibition of Candida utilis NRRL Y-660 took place in molasses stored at 60°C for 120 days. The specific growth rate (μmax) was reduced from 0.42 h-1 to 0.200 h-1 as a result of a lack of affinity from the microorganism to the substrate and the increasing maintenance necessities. The Ks values arose from 1.40 mg/ml to 4.28 mg/ml within the whole experiment. At the same time, the maintenance coefficient (m) increased from 0.250 to 3.80 mg/ml. In a continuous culture the “wash-out” conditions were reached at dilution rate values (D) close to 0.40 h-1. The process productivity decreased up to 15% from its original value in fresh molasses.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140109
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  • 8
    Electronic Resource
    Electronic Resource
    Nucleic acid reduction from yeast activation of intracellular rnase (1985)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 5 (1985), S. 91-100 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The study of a heat-shock process for RNA reduction was carried out for different yeast strains. Different results were obtained from each of them. Candida utilis NRRL Y-660 shows its best performance after a 8-s. heat-shock in the presence of 3% NaCl. For commercial baker's yeast Saccharomyces cerevisiae and Kluyveromyces fragilis L-1930, similar results were obtained with only 1% of NaCl. The latter needed longer heat-shock periods. e.g. 15s. to give such an RNA reduction. Biomass recovery ranged from 60 to 75%, being higher for C. utilis and K. fragilis while excessive losses were observed in S. cerevisiae cells.No significant protein deterioration was obtained in the best performance samples. The aminoacid profile appears to be improved in comparison to the starting material in these strains after RNA reduction.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370050116
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