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  • 1
    Electronic Resource
    Electronic Resource
    Isolation, cDNA cloning and expression of Lig v 1, the major allergen from privet pollen (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 26 (1996), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background An olive allergen-like protein has been detected in privet pollen. This protein could be involved in the allergenic cross-reactivity described for privet and olive tree pollen extracts.Objective Isolation and characterization of natural Lig v 1. Cloning and expression of its cDNA in order to assess its structural similarity with the olive allergen.Methods Current chromatographic methods were used to isolate the privet counterpart of Ole e 1. A pool of sera from subjects allergic to olive tree pollen was used to immunodetect the protein in the elution profiles. Ole e 1-specific polyclonal antibody and allergic sera were used in immunoblotting assays of the isolated protein, Polymerase chain reaction amplification of the first strand cDNA synthesized from the privet pollen total RNA was carried out to prepare a full-length fragment encoding Lig v 1. After nucleotide sequencing, expression of one clone was performed in Escherichia coli, under the form of a fusion protein with glutathione S-transferase. The IgE binding capability of the recombinant protein was also analysed.Results The major allergen from privet pollen, Lig v 1, was purified to homogeneity by two gel filtration chromatographies and one reverse-phase high-performance liquid chromatography. Its amino acid composition and N-terminal amino acid sequence were determined. Two different clones encoding Lig v 1 were sequenced. Strong sequence similarity between Lig v 1 and Ole e 1 was observed, the identity being 85 and 96%. One of the sequenced clones was expressed and the recombinant product exhibited IgG and IgE binding activities against both anti-Ole e 1 polyclonal antibodies and olive-allergic sera.Conclusion Privet pollen contains a protein structurally and immunologically related to the major allergen of ohve pollen. The similarity exhibited by these proteins could explain the cross-reactivity observed between the two pollen extracts. Since these allergens are highly polymorphic, the expression of an immunologically active recombinant Lig V 1 will permit the preparation of well defined molecules for both research and chnical purposes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1996.tb00542.x
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  • 2
    Electronic Resource
    Electronic Resource
    Detection, isolation and complete amino acid sequence of an aeroallergenic protein from rapeseed flour (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 27 (1997), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Seed proteins have been found to cause hypersensitivity by ingestion or inhalation. Rapeseed fiour was responsible for allergic symptoms in a patient, who develops into allergy to mustard spice.Objective To determine the presence of allergenic proteins in rapeseed fiour, and analyse the structure of the main component and its crossreactivity with the mustard allergeti.Methods SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and subsequent immunoblotting with a serum from a rapeseed allergic patient were performed to detect IgE-binding proteins. Proteolytic digestiotis and high performance liquid chromatography were used to obtain the peptides from the allergenic BnIII napin from rapeseed flour. Automatic Edman degradations were carried out to determine their amino acid sequences, which were compared with other sequences in nucleotide and amino acid sequence databases. Crossreactivity assays were carried out by ELISA inhibition using sera from a rapeseed allergic patient and from patients allergic to mustard.Results The 2S albumins of rapeseed were recognized by the serum from a patient allergic to this seed. The most abundant isoform of the allergenic napins, Bnlll, was used for structural and immunological analysis. The protein consists of two different chains of 9.5 and 4,5 kDa. Their complete amino acid sequences were determined. The protein exhibited structural relationships with other napin-like storage proteins from seeds. IgE and IgG crossreactivity between rapeseed and mustard allergens was also demonstrated. Considering the structural and immunological data, certain polypeptide regions are suggested to be involved in the allergenicity of these proteins.Conclusions Rapeseed contains 2S storage proteins which may cause allergy in hypersensitive individuals. These proteins exhibit great sequence similarity with 2S albumins from different seeds. Crossreactivity between mustard and rapeseed flours can be explained by sequetice homology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.1997.660843.x
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  • 3
    Electronic Resource
    Electronic Resource
    Echocardiographic evaluation of patients with rheumatoid arthritis (1983)
    Springer
    Clinical rheumatology 2 (1983), S. 381-385 
    Details
    ISSN: 1434-9949
    Keywords: Echocardiography ; Rheumatoid Arthritis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mode M echocardiograms in 34 consecutive adult RA patients revealed no significant differences with the findings obtained in 18 normal controls of similar age and sex. One patient had an 11 mm separation between the anterior chest wall and the right ventricle suggestive of a 350 ml pericardial effusion but had no corresponding findings between the posterior left ventricular wall and the pericardium. In this patient the electrocardiogram showed low voltage and the chest roentgenogram an enlarged cardiac silhouette. The differences between these series and other series reported in the literature may reflect ethnic and therapeutic differences between the respective RA populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02041559
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  • 4
    Electronic Resource
    Electronic Resource
    A highly active silica(silicon)-supported vanadia catalyst for C1 oxygenates and hydrocarbon production from partial oxidation of methane (1995)
    Springer
    Catalysis letters 33 (1995), S. 279-289 
    Details
    ISSN: 1572-879X
    Keywords: gas phase ; methane partial oxidation ; vanadia/silica-silicon ; C1 oxygenates ; C2+ hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A very low surface area silica-silicon substrate has been used as a support for vanadium oxide and has been tested in the partial oxidation of methane. Use of a reactor with variable dead volume ahead of the bed of the catalyst allows determining the relevance of gas phase reactions in initiating methane conversion. Experimental evidence supports that at atmospheric pressure C1 oxygenates are essentially produced on the catalyst surface rather than in the gas phase. Comparison with a high surface area silica-supported vanadium oxide catalyst clearly highlights the double role of surface area in promoting catalytic activity, but also in promoting non-selective further oxidation of reaction products. It is shown that a reaction system combining dead volume upstream the bed of the catalyst and a very low surface area is very promising to activate methane conversion to C1 oxygenates and C2+ hydrocarbons at remarkable TOF number preventing further non-selective oxidation. In addition, production of C2+ hydrocarbons is observed at temperatures as low as 750 K.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00814231
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  • 5
    Electronic Resource
    Electronic Resource
    An XPS and reduction study of PrCoO3 (1988)
    Springer
    Journal of materials science 23 (1988), S. 1018-1023 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The perovskite-type oxide PrCoO3 has been studied by means of X-ray photoelectron spectroscopy (XPS), reduction in H2 and X-ray diffraction. Two types of oxygen were detected: lattice oxygen (binding energy = 528.4 eV) and adsorbed oxygen (binding energy = 530.9 eV). The increase in relative intensity of the peak corresponding to the latter species after reduction of PrCo03 to 3e− per molecule is assigned to the formation of hydroxyl groups. Temperature-programmed reduction (TPR) results showed two reduction steps: to 1 e− per molecule (Co3.1 → Co2+) at 475 to 635 K, and to 3e− per molecule (Co21 → Co0) at 725 to 815 K. Reduction in the first and second steps occurs according to the contracting sphere model and the nucleation mechanism, respectively. Reduction of Co3+ to Co2+ causes minimal structural changes in the perovskite. Reduction to 3e− per molecule yielded Pr2O3 and metallic cobalt. After this reduction and reoxidation at 973 K, the perovskite structure was regained. By XPS and TPR it was shown that PrCo03 is more easily reducible than LaCo03. It is concluded that the cation in the A position of the structure plays a significant role in the bulk and surface properties of LnCo03 (Ln, lanthanide elements) oxides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01154005
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  • 6
    Electronic Resource
    Electronic Resource
    The influence of MAILLARD's reaction in sugar cane molasses on the kinetic fermentation parameters of Candida utilis NRRL Y-660 (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 53-59 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growth inhibition of Candida utilis NRRL Y-660 took place in molasses stored at 60°C for 120 days. The specific growth rate (μmax) was reduced from 0.42 h-1 to 0.200 h-1 as a result of a lack of affinity from the microorganism to the substrate and the increasing maintenance necessities. The Ks values arose from 1.40 mg/ml to 4.28 mg/ml within the whole experiment. At the same time, the maintenance coefficient (m) increased from 0.250 to 3.80 mg/ml. In a continuous culture the “wash-out” conditions were reached at dilution rate values (D) close to 0.40 h-1. The process productivity decreased up to 15% from its original value in fresh molasses.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140109
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