ISSN:
1573-6903
Keywords:
Neuronal survival
;
apoptosis
;
Heat-Shock Proteins
;
HSP-70
;
NMDA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Cerebellar granule cells (CGC) die apoptotically after five days in culture (DIV) at physiological concentrations of potassium (5 mM; K5). When CGC are depolarized (K25) or treated with NMDA (150 μM) cell survival is increased. CGC changed from K25 to K5 die after 24–48 h. It is known that heat shock protein (HSP) may protect from cell death. Here, we found that cells in K5 showed an increase in HSP-70 levels after 3 DIV. Similarly, in cells changed from K25 to K5, HSP-70 levels were increased after 6 h. Neither NMDA nor K25 treatment affected HSP-70 levels from 2–7 DIV. Ethanol or thermal stress induced HSP-70, but cell survival was not affected in K5 medium. These results suggest that HSP, particularly HSP-70, are not involved in the mechanisms by which NMDA and KCl promote cell survival.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007584802989
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