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  • 1
    Electronic Resource
    Electronic Resource
    Bacteriophage SP01 regulatory proteins directing late gene transcription in vitro (1976)
    [s.l.] : Nature Publishing Group
    Nature 262 (1976), S. 753-757 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Expression of the temporally defined “late” genes of Bacillus subtilis phage SP01 requires the protein products of regulatory genes 33 and 34. These proteins have now been isolated in pure form. Together with a host factor delta, they act synergistically to direct “late” ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/262753a0
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro transcription of a late class of phage SP01 genes (1975)
    [s.l.] : Nature Publishing Group
    Nature 257 (1975), S. 248-251 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 SDS polyacrylamide slab gel electrophoresis of enzyme B, enzyme C, 8 and a. Enzymes B and C were purified as described previously6. 5 and a were dissociated from purified holoenzyme9 from uninfected B. subtilis by chromatography on phosphocellu-lose9, dialysed agamst buffer C (ref. 4) ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/257248a0
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  • 3
    Electronic Resource
    Electronic Resource
    New RNA polymerase from Bacillus subtilis infected with phage PBS2 (1974)
    [s.l.] : Nature Publishing Group
    Nature 252 (1974), S. 21-24 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Bacillus subtilis infected with bacteriophage PBS2 contains a new rifampicin-resistant RNA polymerase that is apparently composed of four different ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/252021a0
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  • 4
    Electronic Resource
    Electronic Resource
    Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis (1982)
    Springer
    Molecular genetics and genomics 186 (1982), S. 339-346 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have determined the nucleotide sequence of twoBacillus subtilis promoters (veg andtms) that are utilized by the principal form ofB. subtilis RNA polymerase found in vegetative cells (σ55-RNA polymerase) and have compared our sequences to those of several previously reportedBacillus promoters. Hexanucleotide sequences centered approximately 35 (the “-35” region) and 10 (the “-10” region) base pairs upstream from theveg andtms transcription startpoints (and separated by 17 base pairs) corresponded closely to the consensus hexanucleotides (TTGACA and TATAAT) attributed toEscherichia coli promoters. Conformity to the preferred -35 and -10 sequences may not be sufficient to promote efficient utilization byB. subtilis RNA polymerase, however, since three promoters (veg, tms andE. coli tac) that conform to these sequences and that are utilized efficiently byE. coli RNA polymerase were used with highly varied efficiencies byB. subtilis RNA polymerase. We have also analyzed mRNA sequences in DNA located downstream from eightB. subtilis chromosomal and phage promoters for nucleotide sequences that might signal the initiation of translation. In accordance with the rules of McLaughlin, Murray and Rabinowitz (1981), we observe mRNA nucleotide sequences with extensive complementarity to the 3′ terminal region ofB. subtilis 16S rRNA, followed by an initiation codon and an open reading frame.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00729452
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  • 5
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of a promoter recognized by Bacillus subtilis RNA polymerase (1980)
    Springer
    Molecular genetics and genomics 180 (1980), S. 57-65 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report the nucleotide sequence of a promoter recognized by RNA polymerase from the gram-positive bacterium Bacillus subtilis. This promoter, which was isolated from B. subtilis phage SP01 DNA, is homologous to promoters for Escherichia coli RNA polymerase; the sequences of the “-35 region” and the “Pribnow box” were 5′TTGACT and 5′CATAAT, respectively (T is the thymine analog 5-hydroxymethyluracil in SP01 DNA). These sequences each differed by only a single base pair from the preferred sequences for E. coli promoters. Not surprisingly, the SP01 promoter was actively transcribed in vitro by E. coli RNA polymerase as well as by B. subtilis RNA polymerase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267352
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