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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 16 (1969), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Groups of albino rats were fed low and high protein diets and the former group was fed with different supplements. The supplements used were niacin, pyridoxine and glutamic acid. A low protein diet was found to decrease the activities of l-glutamate-NAD-oxidoreductase and l-glutamate-1-carboxy-lyase in the cerebrum; this confirmed the results of previous work in this laboratory. Addition of glutamic acid to a low protein diet was found to reverse the effects of a low protein diet to some extent.Groups of animals were fed kodri (Paspalum scorbiculatum), a millet deficient in lysine, with or without lysine itself or foodstuffs rich in it, e.g. moth bean (Phaseolus aconitifolius Jacq.), peas (Pisum sativuni) and skim milk powder. When combined, the groups fed on the lysine or lysine-source supplemented diets were found to perform better in a water maze on the basis of the relative ease with which they could reverse a previous learned pattern. They also had higher activities of l-glutamate-NAD-oxidoreductase and l-glutamate-1-carboxy-lyase than groups with no lysine or lysine source added. There was no significant difference between the groups receiving the different lysine supplements.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1335
    Keywords: Effusions ; Jackfruit lectin ; Diagnostic cytology ; Malignancy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Distinguishing reactive mesothelial cells from adenocarcinoma cells in serous effusions on the basis of morphological criteria alone is often difficult. Interest has therefore been focused on identifying reliable methods to supplement the conventional cytological techniques. Plant lectins have been reported as diagnostic markers for malignant cells. We studied 51 aspirated samples of benign and malignant effusions using horseradish-peroxidase-conjugated jackfruit lectin. No significant difference was observed between the cells of pleural and peritoneal fluids. The reactively proliferated mesothelial cells of benign effusions showed a predominence of mild staining while moderate and intense staining was predominant in malignant effusions. Intense and irregular lectin binding was observed in macrophages irrespective of the cause of effusion. The lectin staining method therefore appears to have some clinical significance as an additional diagnostic aid for use in effusion cytology.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1572-9699
    Keywords: growth temperature ; cell age ; fatty acid composition ; bacillus stearothermophilus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of growth temperature, media composition and cell age on the chemical composition of Bacillus stearothermophilus strain AN 002 has been determined. The total cellular protein decreased and the free amino acid content increased with growth temperature, in both exponential and stationary growth phase. The protein and free amino acid contents of cells were higher in the stationary phase than in the exponential phase, irrespective of growth temperature and media composition. The RNA content was only reduced in cells grown at 55° C. No significant variations were observed in the DNA and carbohydrate contents with respect to growth temperature and cell age. The total lipid and fatty acid compositions on the other hand varied as a function of growth temperature, cell age and media composition. Differences in the relative concentrations of even, odd and branched chain fatty acids were noticed. Novariation was observed in the antiiso and unsaturated fatty acids with respect to growth temperature. The unique variations in the fatty acid composition and total lipids at the growth temperature of 50° C and their variations in the stationary growth phase seem to be characteristic for B. stearothermophilus AN 002.
    Type of Medium: Electronic Resource
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