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  • 1
    Electronic Resource
    Electronic Resource
    Chalcone synthase in rice (Oryza sativa L.): Detection of the CHS protein in seedlings and molecular mapping of the chs locus (1996)
    Springer
    Plant molecular biology 32 (1996), S. 735-743 
    Details
    ISSN: 1573-5028
    Keywords: chalcone synthase cDNA (chs cDNA) ; RFLP ; anthocyanins ; flavonoids ; synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chalcone synthase is a key enzyme that catalyses the first dedicated reaction of the flavonoid pathway in higher plants. The chs gene and its protein product in rice has been investigated. The presence of a chalcone synthase (CHS) protein in rice seedlings and its developmental stage-specific expression has been demonstrated by western analysis. The chalcone synthase of rice was found to be immunologically similar to that of maize. A rice cDNA clone, Os-chs cDNA, encoding chalcone synthase, isolated from a leaf cDNA library of an indica rice variety Purpleputtu has been mapped to the centromeric region of chromosome 11 of rice. It was mapped between RFLP markers RG2 and RG103. RG2 is the nearest RFLP marker located at a genetic distance of 3.3 cM. Some segments of chromosome 11 of rice including chs locus are conserved on chromosome 4 of maize. The markers, including chs locus on chromosome 11 of rice are located, though not in the same order, on chromosome 4 of maize. Genetic analysis of purple pigmentation in two rice lines, Abhaya and Shyamala, used in the present mapping studies, indicated the involvement of three genes, one of which has been identified as a dominant inhibitor of leaf pigmentation. The Os-chs cDNA shows extensive sequence homology, both for DNA and protein (deduced), to that of maize, barley and also to different monocots and dicots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020214
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  • 2
    Electronic Resource
    Electronic Resource
    Transposable elements of maize (1983)
    Springer
    Molecular genetics and genomics 192 (1983), S. 21-31 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The maize Enhancer transposable element can be targeted to any locus and many unstable alleles have been isolated. Normally, the result is an autonomously controlled allele leading to the mutability expression that is coordinately inherited with this locus. In this study, six alleles at the c locus (c-m) autonomously controlled by En, gave rise to a modified expression (original pattern of mutability, coarse is modififed to fine) that is under the control of a factor, segregating independently. This factor modifies the coarse pattern to fine by delaying the early occurring mutation events (c→C) during kernel development leading to the appearance of relatively sharp colored spots. This factor shows En-like activity but is limited to the pattern differentiatio of any one of the progenitor c-m alleles. These source c-m alleles are not affected by the standard En. A model is presented to support the hypothesis that this factor is a modified En with an altered mutator (M) effect and thus delays the M effect on the coarse allele. This altered En allele can be considered as En-Malt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327642
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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