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  • 1
    Electronic Resource
    Electronic Resource
    The Influence of “Slip” on Rheological Measurements on a Mycelial Broth of Aspergillus niger (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 506 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb23853.x
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  • 2
    Electronic Resource
    Electronic Resource
    Phenol degradation by a psychrotrophic strain of Pseudomonas putida (1991)
    Springer
    Applied microbiology and biotechnology 34 (1991), S. 539-543 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Cell growth and phenol degradation kinetics were studied at 10°C for a psychrotrophic bacterium, Pseudomonas putida Q5. The batch studies were conducted for initial phenol concentrations, So, ranging from 14 to 1000 mg/1. The experimental data for 14〈=So〈=200 mg/1 were fitted by non-linear regression to the integrated Haldane substrate inhibition growth rate model. The values of the kinetic parameters were found to be: μm=0.119 h−1, K S=5.27 mg/1 and K I=377 mg/1. The yield factor of dry biomass from substrate consumed was Y=0.55. Compared to mesophilic pseudomonads previously studied, the psychrotrophic strain grows on and degrades phenol at rates that are ca. 65–80% lower. However, use of the psychrotrophic microorganism may still be economically advantageous for waste-water treatment processes installed in cold climatic regions, and in cases where influent waste-water temperatures exhibit seasonal variation in the range 10–30°C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00180585
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  • 3
    Electronic Resource
    Electronic Resource
    Kinetics of the simultaneous batch degradation of p-cresol and phenol by Pseudomonas putida (1988)
    Springer
    Applied microbiology and biotechnology 29 (1988), S. 599-604 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The kinetics of the batch degradation of phenol and p-cresol in a dual-substrate system by Pseudomonas putida was investigated. A model was developed which was able to successfully predict phenol and p-cresol levels during batch growth. A constant specific growth rate (μ) was assumed together with the assumption that the biomass yield factors (Y) for growth on phenol and p-cresol were identical. These simplifying assumptions allowed an analytical solution to the model equations to be easily obtained, as well as reducing the number of unknown parameters that required estimation. Phenol and p-cresol were metabolized simultaneously by P. putida. P. putida did not appear to metabolize either of the two substrates preferentially. The rate of utilization of aparticular organic substrate was related to its fraction of the total organic substrate present, i.e., for substrate 1 the assimilation rate depended on S 1/(S 1+S 2), where S iis the concentration of the ith substrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260991
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  • 4
    Electronic Resource
    Electronic Resource
    Enhanced enzymatic hydrolysis of lignocellulosic biomass pretreated by low-pressure steam autohydrolysis (1985)
    Springer
    Biotechnology letters 7 (1985), S. 531-536 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The use of low-pressure steam autohydrolysis in the pretreatment of corn stover and hybrid poplar has been assessed. In terms of yield of prehydrolyzed solids, minimal by-product formation and extent of subsequent enzymatic saccharification, the results of low-pressure steam pretreatment were found to be as good as or better than those reported for more severe pretreatment processes. Almost complete saccharification of the cellulose in the prehydrolyzed biomass solids was obtained within 24h with a commercial cellulase preparation — Celluclast. The presence of grinding elements (glass beads) during the enzymatic hydrolysis was found to increase the extent of saccharification by 40% to 50% over controls without any grinding elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01199874
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  • 5
    Electronic Resource
    Electronic Resource
    An inexpensive system to provide sparged aeration to shake flask cultures (1995)
    Springer
    Biotechnology techniques 9 (1995), S. 665-670 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary We have constructed a durable and inexpensive aeration system for providing sparged aeration of bacterial cultures grown in shake flasks. A detailed description of the apparatus is presented, sufficient to allow other researchers to construct such a system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00156353
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  • 6
    Electronic Resource
    Electronic Resource
    Disruption of Saccharomyces cerevisiae using enzymatic lysis combined with high-pressure homogenization (1990)
    Springer
    Biotechnology techniques 4 (1990), S. 329-334 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The disruption of commercially-available pressed Bakers' yeast (Saccharomyces cerevisiae) was studied using a relatively new high-pressure homogenizer (the Microfluidizer). Initial experiments using only mechanical disruption generally gave low disruption yields (i.e., less than 40% disruption in 5 passes). Consequently combinations of two disruption methods, namely enzymatic lysis and subsequent homogenization, were tested to identify achievable levels of disruption. The enzyme preparation employed was Zymolyase, which has been shown to effectively lyse the walls of viable yeast. Yeast cell suspensions ranging in concentration from 0.6 to 15 gDW/L were disrupted with and without enzymatic pre-treatment. Final total disruption obtained using the combined protocol approached 100% with 4 passes at a pressure of 95 MPa, as compared to only 32% disruption with 4 passes at 95 MPa using only homogenization. A model is presented to predict the fraction disrupted while employing this novel enzymatic pretreatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00157431
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  • 7
    Electronic Resource
    Electronic Resource
    To the editor (1974)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 20 (1974), S. 1233-1233 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690200634
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  • 8
    Electronic Resource
    Electronic Resource
    Stirred-tank mechanical power requirement and gas holdup in aerated aqueous phases (1977)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 23 (1977), S. 48-56 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Gas holdup and the ratio of the turbulent regime mechanical power consumed in aerated compared to that in nonaerated aqueous phases were measured in two laboratory sized tanks. Standard six-blade turbine (D/T = 1/3), six-blade paddle (D/T = 1/3), and four-blade paddle (D/T = 2/3) impellers were used over a wide range of impeller rotational speed and gas sparging rate. For all systems, the power ratio results were found to fit a semitheoretical correlation (derived from dimensional analysis) involving the impeller Weber number, the aeration number, and the ratio of dispersion and liquid densities. Empirical correlations for gas holdup in water, aqueous solutions of nonelectrolytes, and an aqueous electrolyte solution are given. The overall results lead to the conclusion that power ratio and gas holdup correlations are highly specific to a particular impeller type and are also dependent on the tank size and the liquid phase physicochemical properties.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690230109
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  • 9
    Electronic Resource
    Electronic Resource
    Mass transfer studies of external-loop airlifts and a bubble column (1989)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 393-405 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Gas holdups (∊GR) and liquid-phase volumetric oxygen transfer coefficients (kLaT) were measured for a bubble column (BC) and three different external-circulation-loop airlift (ECL-AL) fermentors of 50 to 60 L working volume, using viscous non-Newtonian aqueous solutions of various carboxymethylcelluloses. Some measurements also were done with a viscous Newtonian system (51.8 wt. % sucrose solution).Discussed in this paper are correlations of ∊GR and kLaT with riser superficial gas velocity (0.02 ≦ UGR ≦ 0.26 m/s), the ratio of the downcomer and riser crosssectional areas (0[BC] ≦ Ad/Ar ≦ 0.444), and the effective viscosity of the liquid phase (0.02 ≦ ηeff ≦ 0.5 Pa · s), over the parameter ranges indicated. It is shown that both ∊GR and KLaT are highly dependent upon UGR and Ad/Ar. The effective viscosity has a significant effect on KLaT, but has only a relatively weak affect on ∊GR. The KLaT correlation developed for non-Newtonian systems was extended to include the results obtained for the viscous Newtonian system studied by incorporating the effects of liquid-phase molecular diffusivity, density, and interfacial tension as determined by Nakanoh and Yoshida (1980).
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690350307
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  • 10
    Electronic Resource
    Electronic Resource
    Simultaneous measurement of interfacial area and mass transfer coefficients for a well - mixed gas dispersion in aqueous electrolyte solutions (1974)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 20 (1974), S. 285-294 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new measuring technique involving concurrent chemical absorption of carbon dioxide and desorption of oxygen is developed for simultaneously evaluating the liquid-phase mass transfer coefficient kL and the specific area a of sparingly-soluble gas dispersions in stirred tanks containing an aqueous solution of inorganic electrolytes. The method ensures that kL and a are evaluated under consistent hydrodynamic conditions.Results from three different nonviscous systems show that at high agitation power, such that the average bubble diameter is between 0.2 and 2 mm, kL decreases with increasing power input and is dependent on the bubble diameter. This behavior is in contrast to the results of others at lower agitation levels or in nonelectrolytic liquids, but in general agreement with previous results for bubbles of the same diameter range produced in viscous, nonelectrolytic solutions.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690200212
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