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  • 1
    Electronic Resource
    Electronic Resource
    Effect of environmental factors on PCR-DNA analysis from dental pulp (1996)
    Springer
    International journal of legal medicine 109 (1996), S. 125-129 
    Details
    ISSN: 1437-1596
    Keywords: DNA polymorphisms ; PCR ; Dental pulp ; Identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract This study was designed to observe the results of DNA typing on teeth subjected to aging, different temperatures and various environmental factors. A total of 570 teeth were studied. The study included the analysis of the PCR-based polymorphisms HLA DQA1, D1S80, HUMTH01, HUMFES/FPS and the XY homologous gene amelogenin. In general the best results were obtained with the XY homologous gene amelogenin, followed by the two STRs studied (HUMTH01 and HUMFES/FPS). The small fragment sizes and the method of detection used after PCR amplification are the main factors explaining this fact. In general, teeth submerged in water gave the poorest results. Teeth exposed to outdoor conditions provided better results than teeth buried in sand or soil, but even in these cases good results were obtained. Up to 4°C, temperature had only a slight influence on the results. Positive results were obtained in most cases at high temperatures (400°C for 2 min) which are rarely reached in practical casework. Positive typing results for the XY homologous gene amelogenin and the STRs were obtained from teeth 10–30 years old. The usefulness of dental pulp for identification purposes is exemplified in some actual cases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01369671
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A review of the collaborative exercises on DNA typing of the Spanish and Portuguese ISFH working group (1997)
    Springer
    International journal of legal medicine 110 (1997), S. 273-277 
    Details
    ISSN: 1437-1596
    Keywords: Key words DNA polymorphisms ; Standardization ; Collaborative exercise ; Forensics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract Since 1992 the Spanish and Portuguese Working Group (GEP) of the International Society for Forensic Haemogenetics (ISFH) has been organizing collaborative exercises on DNA profiling with the aim of making progress on standardization and discussing technical and statistical problems in DNA analysis. A total of four exercises (GEP-92 to GEP-95) have been carried out until now. A consequence of these exercises was the creation of a quality control programme in Spain and Portugal in 1995 which was carried out simultaneously with the GEP-95 exercise. The number of participating laboratories increased from 10 in the first exercise (GEP-92) to 19 in the last exercise (GEP-95). Despite this increasing number of participating laboratories, results remained satisfactory. In the last exercises, all the laboratories used PCR-based DNA polymorphisms with an increasing number of markers obtaining good results. SLPs were used by only 30% of laboratories in the last two exercises but the results indicated a good level of expertise in most of these laboratories. The reasons for these successful results are the common use of the EDNAP protocol for SLP analysis and commercially available kits or common sequenced allelic ladders for PCR-based DNA polymorphisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140050084
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Plasma protein and red cell enzyme groups in Galicia (North West Spain) (1987)
    Springer
    International journal of legal medicine 98 (1987), S. 133-140 
    Details
    ISSN: 1437-1596
    Keywords: Blood groups, GALT-EsD-PLG-Hp ; GALT-EsD-PLG-Hp gene frequencies, Galicia (Spain) ; Blutgruppen, GALT-EsD-PLG-Hp ; GALT-EsD-PLG-Hp Genfrequenzen, Galizien (Spanien)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Die Phänotypen der GALT, EsD und PLG wurden durch PAGIF in einer Stichprobe aus Galizien bestimmt. Hp wurden durch konventionelle Elektrophorese bestimmt. Folgende Genfrequenzen wurden ermittelt: für GALT: GALTN: 0,930; GALTD1: 0,044; GALTD2: 0,025; für EsD: EsD1: 0,874; EsD2: 0,104; EsD3: 0,021; für PLG: PLG1: 0,800; PLG2: 0,199; für Hp: Hp1: 0,426; Hp2: 0,573. Die Ergebnisse der Bevölkerungsdaten aller elektrophoretischen Charakteristika (Markers), die bisher innerhalb der Galizischen Bevölkerung ermittelt wurden, wurden ebenfalls berücksichtigt.
    Notes: Summary Galactose-1-phosphate uridyl transferase (GALT), esterase D (EsD), and plasminogen (PLG) phenotypes were determined by isoelectric focusing in thin-layer polyacrylamide gels (PAGIF) in a random sample from Galicia. Haptoglobins (Hp) were determined by conventional electrophoresis. The following gene frequencies were observed: for GALT: GALTN: 0.930; GALTD1: 0.044; GALTD2: 0.025; for EsD: EsD1: 0.874; EsD2: 0.104; EsD3: 0.021; for PLG: PLG1: 0.800; PLG2: 0.199; for Hp: Hp1: 0.426; Hp2: 0.573. Population data results of all electrophoretic markers typed until now in Galician population are also included.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00200471
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  • 4
    Electronic Resource
    Electronic Resource
    Determination of phosphoglucomutase (PGM1), acid phosphatase (ACP), and esterase D (ESD) in human bloodstains by hybrid isoelectric focusing (HIEF) (1989)
    Springer
    International journal of legal medicine 102 (1989), S. 271-275 
    Details
    ISSN: 1437-1596
    Keywords: Blood groups, PGM1, ESD, ACP ; Bloodstains, PGM1, ESD, ACP determination by HIEF ; Blutgruppen, PGM1, ESD, ACP ; Blutspuren, Nachweis von PGM1, ESD, ACP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung PGM, ESD, and ACP werden in Blutspuren durch IEF mit CA und durch HIEF bestimmt. Das HIEF zeigt bessere Ergebnisse bei der Typisierung von PGM in Blutspuren während sich für die Typisierung von ESD und ACP die IEF mit als bessere Methode erweist.
    Notes: Summary PGM1, ESD, and ACP were determined in bloodstain extracts by isoelectric focusing (IEF) with carrier ampholytes (CA) and HIEF. HIEF yields superior results in PGM typing from bloodstain extracts, whereas for ESD and ACP typing isoelectric focusing with carrier ampholytes seems to be the method of choice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201323
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  • 5
    Electronic Resource
    Electronic Resource
    Y chromosome STR haplotypes: genetic and sequencing data of the Galician population (NW Spain) (1998)
    Springer
    International journal of legal medicine 112 (1998), S. 15-21 
    Details
    ISSN: 1437-1596
    Keywords: Key words Y chromosome haplotypes ; STRs ; Sequencing data ; Galician population
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract Recently described Y-STR polymorphisms can be analysed as informative haplotypes which are useful in the forensic field. In order to include these systems in our forensic routine, we have carried out a population study in Galicia (NW Spain) analysing seven Y-STR polymorphisms (DYS19, DYS389-I, DYS389-II, DYS390, DYS393 and DYS385: two loci). The results were compared with other population studies. In addition various alleles for each system (except DYS385) were sequenced and the corresponding allelic ladders constructed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140050191
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    Paper (German National Licenses)
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