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  • 1
    Electronic Resource
    Electronic Resource
    Effect of etoposide on experimental testicular teratoma in 129/SvJ mice (2000)
    Springer
    Virchows Archiv 436 (2000), S. 608-616 
    Details
    ISSN: 1432-2307
    Keywords: Key words Testis ; Neoplasms ; Tissue culture ; Differentiation ; Etoposide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  To study the effects of etoposide on experimental testicular teratoma in 129/SvJ mouse we analysed the tumour growth, differentiation, apoptosis and the localisation of mdr1 P-glycoprotein (mdr1-Pgp). In this model the implanted gonadal ridges developed into testicular teratomas in 17 out of 56 implanted testes (30%) and in 14 out of 28 mice (50%). The tumour-bearing mice were treated with etoposide on 4 successive days either 4 weeks or 6 weeks after implantation, and killed 7 days after the last dose. The mice in the control groups did not receive etoposide. The teratomas consisted mainly of neural tissue. The etoposide-treated 4-week teratomas, but not the 6-week teratomas, were significantly smaller than those in the corresponding control groups. The density of apoptotic cells and the distribution of the mdr1-Pgp were not altered by etoposide. The decreased proportion of immature neuroectodermal tissue components was observed in all treated teratomas, converting the histology towards that of a mature teratoma. In addition, a low proportion of immature tissue components was frequently combined with a low density of apoptotic cells. In conclusion, etoposide decreased the immature tissue components of teratomas, while mature tissues remained unaffected. These results may have clinical relevance in man, since they confirm that postchemotherapy mature teratomas cannot be treated with chemotherapy. Despite benign histology, the human residual tumours have a significant malignant potential and require complete surgical excision and close surveillance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004280000194
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  • 2
    Electronic Resource
    Electronic Resource
    Abdominopelvic actinomycosis associated with intrauterine devices. Two case reports (1988)
    Springer
    Archives of gynecology and obstetrics 243 (1988), S. 237-241 
    Details
    ISSN: 1432-0711
    Keywords: Intrauterine devices (IUD) ; Actinomycosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two case reports of abdominopelvic actinomycosis associated with an intrauterine device (IUD) are presented. In the first case, the association was difficult to establish and in the second one, a pelvic malignancy was suspected. The diagnosis and treatment of IUD-associated abdominopelvic actinomycosis are discussed on the basis of the present cases and the literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00932275
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  • 3
    Electronic Resource
    Electronic Resource
    Early detection of cell damage by supravital acridine orange staining (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 265-266 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell damage can be detected in living cells by acridine orange fluorescence earlier than with phase contrast microscopy or with conventional histological methods. The change in the acridine orange fluorescence from green to red indicates that the secondary structure of the DNA is altered very early during the cell death.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02124103
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  • 4
    Electronic Resource
    Electronic Resource
    Binding of fluorescein isothiocyanate conjugated lectins to rat spermatogenic cells in tissue sections (1984)
    Springer
    Histochemistry and cell biology 80 (1984), S. 575-579 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The testes from three months old Spague-Dawley rats were fixed in Bouin's fluid or neutral buffered 10% formalin, embedded in paraffin, sectioned and after deparaffination stained with the following fluorescein isothiocyanate coupled lectins: PNA, WGA, Con A, RCA, SBA, DBA and UEA. The results show that there are considerable differences in the staining pattern of various spermatogenic cells between different lectins. The fixation in Bouin's fluid enhanced the staining of all the lectins compared to formalin fixation in which only a weak staining could be seen in the acrosomes of spermatids after WGA or PNA staining. PNA and WGA stained specifically the acrosome of the developing spermatids, which was seen from the beginning of the acrosome formation and lasted up to late spermiogenesis. However, the staining with PNA decreased in the late spermatids whereas the intensity of the staining remained unchanged with WGA. Con A did not stain the acrosome but stained unspecifically the cytoplasm of all spermatogenic cells. RCA stained faintly the acrosome throughout the spermatid differentiation. DBA and UEA stained specifically the chromosomes of B spermatogonia. DBA also faintly stained the cell membranes of early spermatids. SBA did not show any specific staining of the spermatogenic cells. Based on this it is suggested that the carbohydrates and glycoproteins which are known to be present in the acrosome are formed already in the beginning of the acrosome formation. The decrease in the PNA staining in late spermatids possibly reflects the fact that the receptor molecules are not synthesized in late spermatids but are formed in earlier developmental stages and are thereafter preserved in the acrosome. The enhancement of lectin binding caused by Bouin's fixative might also be applied to other tissues where previous experiments with formalin fixed tissue have failed to show any staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02400975
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  • 5
    Electronic Resource
    Electronic Resource
    Lectin binding to collagen strands in histologic tissue sections (1987)
    Springer
    Histochemistry and cell biology 87 (1987), S. 557-560 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histologic sections from human skin and uterine ligaments were stained with the following FITC conjugated lectins: Con A, WGA, s-WGA, SBA, DBA, UEA I, PNA, RCA I, BPA, GSA I, GSA II, MPA and LPA. The staining of the connective tissue was similar in the dermis and the uterine ligaments and it was most intense in the extracellular matrix containing collagen strands whereas the fibrocytes remained unstained. The staining was clear with glucose or N-acetylglucosamine binding lectins like Con A, WGA, s-WGA and GSA II, which may be related to the presence of glucose residues in collagenous hydroxylysine. The staining with some of the galactose or N-acetylgalactosamine binding lectins like RCA I, DBA, and BPA was less intense. This may reflect the presence of terminal galactose sugars in the hydroxylysine of collagen. No staining was found with SBA, UEA I, PNA, GSAI, MPA or LPA. The results show that different particularly glucose specific lectins bind to the extracellular matrix and especially to collagenous strands in connective tissue. It is suggested that this might be used in histochemical studies of connective tissue and particularly concerning the changes that may occur in different disease states.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492470
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  • 6
    Electronic Resource
    Electronic Resource
    Binding of fluorescein isothiocyanate conjugated lectins to rat spermatogenic cells in tissue sections (1984)
    Springer
    Histochemistry and cell biology 80 (1984), S. 475-579 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The testes from three months old Spague-Dawley rats were fixed in Bouin's fluid or neutral buffered 10% formalin, embedded in paraffin, sectioned and after deparaffination stained with the following fluorescein isothiocyanate coupled lectins: PNA, WGA, Con A, RCA, SBA, DBA and UEA. The results show that there are considerable differences in the staining pattern of various spermatogenic cells between different lectins. The fixation in Bouin's fluid enhanced the staining of all the lectins compared to formalin fixation in which only a weak staining could be seen in the acrosomes of spermatids after WGA or PNA staining. PNA and WGA stained specifically the acrosome of the developing spermatids, which was seen from the beginning of the acrosome formation and lasted up to late spermiogenesis. However, the staining with PNA decreased in the late spermatids whereas the intensity of the staining remained unchanged with WGA. Con A did not stain the acrosome but stained unspecifically the cytoplasm of all spermatogenic cells. RCA stained faintly the acrosome throughout the spermatid differentiation. DBA and UEA stained specifically the chromosomes of B spermatogonia. DBA also faintly stained the cell membranes of early spermatids. SBA did not show any specific staining of the spermatogenic cells. Based on this it is suggested that the carbohydrates and glycoproteins which are known to be present in the acrosome are formed already in the beginning of the acrosome formation. The decrease in the PNA staining in late spermatids possibly reflects the fact that the receptor molecules are not synthesized in late spermatids but are formed in earlier developmental stages and are thereafter preserved in the acrosome. The enhancement of lectin binding caused by Bouin's fixative might also be applied to other tissues where previous experiments with formalin fixed tissue have failed to show any staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00553720
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  • 7
    Electronic Resource
    Electronic Resource
    Differentiation-related changes in the distribution of glycoconjugates in rat testis (1990)
    Springer
    Histochemistry and cell biology 94 (1990), S. 387-395 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of glycoconjugates in differentiating rat testis was investigated by fluorescein labeled lectins during embryogenesis and postnatal development. Double immunofluorescence with rhodamine coupled laminin antibodies was used to delineate testicular cords from the interstitium in embryonic testes. Rat testis was found to be rich in various glycoconjugates, with distinct differentiation-related changes in their distribution. All types of germ cells contained carbohydrate rich compounds in their cytoplasm. Glycosylation in the embryonic testis was different from that in the adult rat. At an early stage of testicular differentiation, the labeling of germ cells and other testicular cells was almost identical. The lectin binding patterns of embryonic germ cells and somatic cells were related to the developmental age of the animal, with a graded disappearance of galactose containing glycoconjugates in embryonal spermatogonia. Spermatogenic cell differentiation was characterized by striking changes in lectin binding patterns of germ cells, particularly in the acrosomes of developing spermatids, in relation to their functional activation and the emergence of adult type of glycosylation during the postnatal maturation of the testis. As the knowledge of regular glycosylation throughout tissue differentiation is of significance for the analysis of aberrant glycosylations occurring in pathologic disorders, our findings suggest the usefulness of lectin histochemistry for the studies on germ cell differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266445
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  • 8
    Electronic Resource
    Electronic Resource
    Correlation between lectin binding and clinical factors in seminoma patients (1989)
    Springer
    Journal of cancer research and clinical oncology 115 (1989), S. 96-100 
    Details
    ISSN: 1432-1335
    Keywords: Seminoma ; Lectin ; Lectin histochemistry ; Testis ; Cryptorchism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The binding of a panel of lectins to histological sections of seminomas was studied. The findings were correlated with different clinical parameters. Concanavalin A and wheat germ agglutinin stained all seminomas whereas horse gram (DBA) and peanut agglutinin did not stain the tumor cells. A varying staining pattern was found with lectins from castor bean (RCA I), soy bean (SBA) and gorse (UEA I) indicating a heterogeneity of the tumor cell population. In the seminomas that were derived from undescended testis there were more cases that showed positive staining with soy bean agglutinin, which shows that the intra-abdominal location of the seminoma might cause changes in the cellular metabolism resulting in glycoconjugates different from those in descended tumors. No correlation was found between the lectin staining and the prognosis, stage or metastasis of the tumor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391608
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  • 9
    Electronic Resource
    Electronic Resource
    Should i.m. injections be avoided during cancer chemotherapy? (1988)
    Springer
    Journal of cancer research and clinical oncology 114 (1988), S. 531-532 
    Details
    ISSN: 1432-1335
    Keywords: Intramuscular injection ; Cancer chemotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The intravascular transit of malignant tumor cells constitutes an important step in the formation of distant metastases. The development of tumors in extravascular tissues depends upon the exit of these cells from the circulation by crossing the barriers formed by endothelium and basement membrane and their growth in the extravascular environment. Cytostatic drugs may disturb the function of these barriers and some of them, as well as antiemetic drugs, are given as i.m. injections. Thus these both mechanically and chemically induced endothelial and extravascular tissue changes might facilitate tumor cell transit to extravascular tissues. These aspects are discussed in this case report of a young female patient with a mediastinal germ cell tumor. At 4 years after complete remission induced with chemotherapy and radiotherapy she developed recurrent germ cell tumor in the form of a s.c. gluteal abscess. This gluteal region is the most common site of i.m. injections during or after cancer chemotherapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391508
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  • 10
    Electronic Resource
    Electronic Resource
    Tumor localization with18F-2-fluoro-2-deoxy-D-glucose: Comparative autoradiography, glucose 6-phosphatase histochemistry, and histology of renally implanted sarcoma of the rat (1985)
    Springer
    Research in experimental medicine 185 (1985), S. 87-94 
    Details
    ISSN: 1433-8580
    Keywords: Glucose metabolism ; Malignant tumors ; Glucose 6-phosphatase ; Autoradiography ; Enzyme histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rous sarcoma cells were implanted into the kidney of rats. After 5 days of growth the renal tumor was used for comparing histology with glucose 6-phosphatase (G6Pase) enzyme histochemistry (EHC) and18F-fluoro-2-deoxyglucose (FDG) auroradiography (ARG). It was found that the regions of the kidney tumor that had retained normal kidney structures were devoid of FDG, whereas there was histochemical staining of normal cortical areas. Regions of tumor growth, on the other hand, retained FDG and lacked G6Pase. Necrotic areas did not accumulate FDG. There was a dramatic decrease in the areas of G6Pase activity as a result of tumor infiltration in the kidney. The results show that FDG, currently being evaluated as a tumor detecting radiopharmaceutical indeed accumulates into areas of vital malignant growth, and they indicate that FDG positron emission tomographic (PET) images reveal the true anatomic location of malignant tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01854893
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