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  • 1
    Electronic Resource
    Electronic Resource
    Hypolimnetic phosphorus retrieval by diel vertical migrations of lake phytoplankton (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 14 (1984), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. I. Movement of 33P from hypolimnion to epilimnion in a small, dystrophic lake was investigated using small-diameter experimental tubes enclosing thermally stratified water columns. This approach was made possible by the extremely sharp stratification found in such lakes, in which the euphotic zone closely coincides with the epilimnion.2. The vertical distribution of inorganic phosphorus in the lake showed a sharp increase across the thermocline so that enhanced concentrations were available to phytoplankton just below the thermocline. Inorganic nitrogen concentrations did not show such a marked relation to thermal stratification.3. One abundant motile alga (Cryptomonas marssonii) showed striking and regular vertical migrations in the lake, moving below the thermocline at night and returning to the surface waters in early morning. These migrations took cells across a 10°C temperature gradient. Non-motile phytoplankton showed constant vertical distributions.4. In the experimental tubes an upward movement of phosphorus took place from hypolimnion to epilimnion which was only attributable to transport by phytoplankton cells undertaking active vertical migrations. No equivalent movement of phosphorus occurred in control tubes from which algae were absent.5. The possible significance of such nutrient retrieval is discussed with reference to plankton phosphorus budgets and competition between phytoplankton species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2427.1984.tb00165.x
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  • 2
    Electronic Resource
    Electronic Resource
    Phosphorus transformations in the epilimnion of humic lakes: abiotic interactions between dissolved humic materials and phosphate (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 19 (1988), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 1. Sephadex gel filtration of filtered water from small, Finnish forest lakes demonstrated abiotic movement of 33P from added PO4 to two higher molecular weight fractions. This movement was most pronounced in waters with high humic content which also had high iron content. The two fractions which took up 13P had nominal molecular weights of 〉 100,000 and 10,000-20,000.2. An equilibrium existed between free PO4 and the two fractions. However, one fraction, at least, appeared to exist in two phases, with one phase in rapid equilibrium with free PO4 but the other in only slow equilibrium.3. Additions of ferric iron up to 1 mg Fe l−1 to the filtered lake water stimulated movement from free PO4, provided high concentrations of humic materials were present. In the absence of humic materials even 0.1 mg Fe 1−1 would precipitate all added 33PO4.4. The high molecular weight P was only partially reactive with standard molybdate reagents. Exposure of the high molecular weight P to sunlight caused a small release of PO4 under the experimental conditions employed.5. Possible implications for biological phosphorus demand of such sequestration of free PO4 by humic materials in combination with iron are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2427.1988.tb00357.x
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  • 3
    Electronic Resource
    Electronic Resource
    Does ionic strength affect the configuration of aquatic humic substances, as indicated by gel filtration? (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Freshwater biology 17 (1987), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. 1. The effect of ionic strength on the configuration of aquatic humic substances was studied by gel filtration and dialysis of water from small. Finnish forest lakes of varying colour.2. Sephadex gel filtration of water from the most humic lake gave similar elution profiles of UV absorbance and dissolved organic carbon (DOC). Gel filtration of unconcentrated samples from all three lakes under natural conditions of ionic strength (I = 1.7 × 10−4) and pH (5.5–6.0) gave similar fractionation of humic substances, despite their widely differing colour (30–350 mg Pt l−1) and DOC (5–25mg C l−1).3. Increasing the ionic strength by two orders of magnitude caused considerable retardation on the Sephadex columns of the humic substances, suggesting a decrease in their molecular size and/or shape.4. Dialysis experiments strongly indicated that ionic strength-induced changes in the configuration of the aquatic humic substances are indeed real. Hence it is probable that the elution behaviour of aquatic humic substances on Sephadex gels has previously been wrongly attributed to ionic strength-dependent interactions between the gel and the humic substances.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2427.1987.tb01066.x
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  • 4
    Electronic Resource
    Electronic Resource
    Increased type I collagen mRNA levels in cultured scleroderma fibroblasts
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression 781 (1984), S. 183-186 
    Details
    ISSN: 0167-4781
    Keywords: (Scleroderma fibroblast) ; Cell culture ; Collagen synthesis ; collagen mRNA
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4781(84)90136-2
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  • 5
    Electronic Resource
    Electronic Resource
    Urinary glycosaminoglycans in aspartylglycosaminuria: evidence for disturbed proteoglycan metabolism
    Amsterdam : Elsevier
    Clinica Chimica Acta 146 (1985), S. 111-118 
    Details
    ISSN: 0009-8981
    Keywords: Aspartylglycosaminuria ; Glycoprotein storage disorders ; Heparan sulfate ; Urinary glycosaminoglycans
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0009-8981(85)90049-X
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  • 6
    Electronic Resource
    Electronic Resource
    Comparison of posttransfusion recoveries achieved with either fresh or stored platelet concentrates (1987)
    Springer
    Annals of hematology 54 (1987), S. 207-212 
    Details
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effectiveness of platelet concentrate transfusion depends on such variables as blood bag material, donor — recipient compatibility, and time elapsed between donation and transfusion. To study the latter a corrected thrombocyte increment for recovery in the recipients was evaluated with 108 platelet transfusions in 31 patients. In 83 treatment programs, the mean recovery at the one-hour post-transfusion time point was 8.6×109 platelets/l with fresh platelets and 5.9×109 platelets/l with stored platelets. Significantly better recovery was achieved with freshly prepared platelet over the total of platelet concentrates stored for up to 96 hours; however, if the recoveries in different patient groups given stored platelets were considered separately in terms of storage times of up to 48 h or 48–96 h, the good recovery with fresh platelets was significantly better only when compared to the older (p=0.034) but not to the younger group of stored platelets. In patients with signs indicating enhanced platelet destruction (fever, splenomegaly, disseminated intravascular coagulation) the transfusion with fresh platelet concentrates gave a significantly better recovery compared to stored platelet concentrates (p=0.028), whereas in the absence of such signs the recovery produced by fresh concentrates was not significantly higher than with stored concentrates. These findings may be relevant for the logistics in blood banking.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00594195
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  • 7
    Electronic Resource
    Electronic Resource
    Therapie und Prognose der oberen Einflußstauung beim anaplastischen kleinzelligen Bronchuscarcinom (1981)
    Springer
    Journal of molecular medicine 59 (1981), S. 385-389 
    Details
    ISSN: 1432-1440
    Keywords: Vena cava ; Superior/carcinoma ; Oat cell/drug therapy ; Obere Einflußstauung ; kleinzelliges Bronchuscarcinom ; Chemotherapie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In einer retrospektiven Studie wurden 149 Patienten mit anaplastischem kleinzelligem Bronchuscarcinom untersucht. 19 Patienten (13%) wiesen bereits initial eine obere Einflußstauung auf, 11 weitere Patienten (7%) entwickelten diese Komplikation im weiteren Verlauf ihrer Krankheit. 16 Patienten, die bereits zu Beginn ihrer Krankheit eine Obstruktion der oberen Hohlvene aufwiesen, wurden mit einer alleinigen Chemotherapie behandelt. 13 Patienten sprachen auf diese Behandlung mit einer teilweisen (7 Patienten) oder vollständigen (6 Patienten) Rückbildung der oberen Einflußstauung an. Wegen einer für heutige Begriffe suboptimalen Therapie entwickelten 7 der 13 Patienten (54%) im Mittel 4,5 Monate nach Therapiebeginn ein Rezidiv. 3 Patienten sprachen auf eine zweite Chemotherapie an, bei 4 Patienten war eine Radiotherapie erfolgreich. Die obere Einflußstauung ist eine häufige Komplikation des anaplastischen kleinzelligen Bronchuscarcinoms und kann mit alleiniger Chemotherapie erfolgreich behandelt werden.
    Notes: Summary One hundred and forty nine consecutive patients with small-cell anaplastic carcinoma of the lung were studied retrospectively. 19 patients (13%) presented with superior vena caval obstruction, 11 further patients (7%) developed this complication during the course of their disease. 16 patients presenting with superior vena caval syndrome were treated with chemotherapy alone and 13 patients responded. Due to suboptimal chemotherapy for current standards 7 of the 13 successfully treated patients (54%) relapsed after a median of 4.5 months. 3 patients were effectively retreated with another chemotherapy combination, 4 patients with radiation therapy. Superior vena caval obstruction is a frequent complication of small-cell anaplastic carcinoma of the lung. It can be treated effectively with combination chemotherapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01698516
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  • 8
    Electronic Resource
    Electronic Resource
    Characterization of the perivascular reticulin network in a case of primary brain lymphoma (1985)
    Springer
    Acta neuropathologica 66 (1985), S. 299-305 
    Details
    ISSN: 1432-0533
    Keywords: Perivascular reticulin network ; Primary brain lymphoma ; Collagen ; Laminin ; Fibronectin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The character of the silver positive reticulin network was analyzed with immunofluorescence and immunoperoxidase methods in an intra vitam diagnosed case of primary brain lymphoma. The network was shown to contain connective tissue proteins rich in hexose-sugars, such as type III collagen (classical “reticulin”), basal lamina constituents type IV collagen and laminin, pericellular type V collagen, as well as fibronectin (protein involved in cell adhesion). On the other hand, very little of the fibrous type I collagen was discernible. Similarly as the silver positive network, the immunohistochemically demonstrable reticulum seemed to hold the cells in the perivascular location, and once it was broken diffuse spread into the tissue occurred. Since malignant cells of B-lymphocyte origin are not known to synthesize so-called reticulin, it is suggested that the network in primary brain lymphomas is produced by cells in the brain parenchyma (possibly pericytes or astrocytes) as a protective attempt to restrict the spread of foreign cells into the brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690962
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  • 9
    Electronic Resource
    Electronic Resource
    Neurofibromatosis tumor and skin cells in culture (1984)
    Springer
    Acta neuropathologica 63 (1984), S. 269-275 
    Details
    ISSN: 1432-0533
    Keywords: Neurofibromatosis ; Cell culture ; Cell surface ; Cytoskeleton ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Structural proteins of cultured neurofibromatosis (NF) tumor and skin cells were studied with reference to control skin fibroblasts. In polyacrylamide gel electrophoresis (PAGE)/fluorography the banding patterns of the cell lysates were markedly similar. NF tumor cells, however, produced a 60 kD band with a stronger and a 48 kD band with a lighter protein staining and metabolic labeling intensity. Furthermore, skin cells were also characterized by a 26 kD protein and the tumor cells by a 22 kD protein with high metabolic labeling intensity. Neuraminidase/galactose oxidase/NaB3H4-labeled NF skin and control skin cells possessed a 220 kD protein that was less intensively labeled in the tumor cells. The banding pattern of the skin cells was also characterized by a protein with slightly lower molecular weight (86 kD) than that of the tumor cell lysates (90 kD). In all cell lines studied indirect immunofluorescence stainings revealed bright arrays of vimentin type intermediary filaments but no desmin, cytokeratin, glial fibrillary acidic protein (GFAP), or neurofilament proteins. NF skin and control skin cells possessed well developed actin-containing bundles of microfilaments, while those of the tumor cells lacked a typical stress-fiber organization. The general morphology of the tumor cell cultures was also irregular. Transmission electron microscopy revealed no basic differences in the structure of intermediary filaments or microfilaments. The present data provide basic knowledge of neurofibromatosis skin and tumor cells and demonstrate that cultured cells originating from neurofibromas are defective in both their intracellular and extracellular organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687332
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  • 10
    Electronic Resource
    Electronic Resource
    Central nervous system involvement in gyrate atrophy of the choroid and retina with hyperornithinaemia (1999)
    Springer
    Journal of inherited metabolic disease 22 (1999), S. 855-866 
    Details
    ISSN: 1573-2665
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In gyrate atrophy of the choroid and retina with hyperornithinaemia (GA), a genetically determined deficiency of ornithine {3}d-aminotransferase activity leads to high ornithine concentrations in body fluids. GA is characterized by centripetally progressing retinal and choroidal destruction and selective atrophy with tubular aggregates in type II skeletal muscle fibres. These findings have been suggested to be mediated by hyperornithinaemia-induced deficiency of high-energy creatine phosphate. As abnormal brain magnetic resonance images and electroencephalograms are found in another disorder of creatine metabolism, guanidinoacetate methyltransferase deficiency, we investigated the central nervous system involvement in GA, which seems to be associated with a milder degree of phosphocreatine deficiency. We compared 23 untreated GA patients with age-matched healthy controls, and with 9 patients who had received creatine or creatine precursor supplementation daily for several years. The mean age of the patients (32±18 years) was similar to that of the controls (36±22 years). The MRI or EEG findings of the patients on creatine supplementation did not differ from those of the untreated group. Brain MRI revealed degenerative lesions in the white matter in 50% of the GA patients, and 70% of the patients had premature atrophic changes, with a striking increase in the number of Virchow's spaces. Of the patients whose EEG was recorded, 58% had abnormal slow background activity, focal lesions or high-amplitude β rhythm (〉50μV). The EEG findings were not associated with the MRI changes or with the age or the sex of the patients. Early degenerative and atrophic brain changes and abnormal EEG are thus features of GA, in addition to the well-characterized eye and muscle manifestations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005602405349
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