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  • 1
    Electronic Resource
    Electronic Resource
    Adsorption of fibronection to Teflon followed byS. aureus adherence (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 90-90 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01975915
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  • 2
    Electronic Resource
    Electronic Resource
    Hepatitis B antigen and systemic lupus erythematosus (1976)
    Springer
    Journal of molecular medicine 54 (1976), S. 303-307 
    Details
    ISSN: 1432-1440
    Keywords: Hepatitis B-Antigen ; Lupus erythematodes disseminatus ; Komplementfixation ; Hepatitis B antigen ; Systemic lupus erythematosus ; Complement fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Sera from 22 patients with systemic lupus erythematosus (SLE) were examined for the presence of hepatitis B antigen (HBsAg) by a complement fixation (CF) test, by an immunoelectrophoretic method (counterelectrophoresis-CEP), and by radioimmunoassay (RIA). The sera from 8 patients gave positive results using CF. However, the same sera and sera from 28 additional SLE patients, when tested with CEP and RIA, were not shown to contain HBsAg. Additional studies were carried out in order to characterize the factor responsible for the false positive CF of the 8 SLE sera. It was shown that the sera also fixed complement in presence of normal serum previously submitted to freezing and thawing or heating at 65°C. The complement fixing factor was readily absorbed by aggregated IgG but not by insolubilized HBsAg. Complement fixation was strongly diminished by 2-mercaptoethanol treatment of SLE serum. It thus appears that the false positive reactions for the presence of HBsAg obtained by CF are due to the occurrence of “anti-antibodies” reacting with aggregated IgG. There is no increased incidence of HBsAg in the serum of SLE patients.
    Notes: Zusammenfassung Seren von 22 Patienten mit Lupus erythematodes disseminatus (LED) wurden auf das Vorliegen des Hepatitis-B-Antigens (HBsAg) untersucht. Zu diesem Zwecke wurden 3 verschiedene Methoden eingesetzt: 1. Komplement-Fixationstest (KFT), 2. Überwanderungselektrophorese (UEP) und 3. Radio-Immunoassay. Seren von 8 Patienten zeigten positive Resultate im KFT. Dieselben Seren und solche von 28 weiteren LED Patienten enthielten kein HBsAg, wenn sie mit UEP und Radio-Immunoassay getestet wurden. Zusätzliche Studien dienten der Charakterisierung des für den positiven KFT verantwortlichen Faktors. Es zeigte sich, daß die LED Seren auch Komplement in Gegenwart von normalen Seren fixierten, wenn letztere mehrfach gefroren und aufgetaut oder auf 65°C erhitzt worden waren. Der Komplement-fixierende Faktor war absorbierbar mit aggregierten Gammaglobulinen, nicht aber mit HBsAg. Die Komplement fixierende Aktivität des Faktors wurde durch Behandlung der Seren mit 2-Merkapto-Aethanol abgeschwächt. Die falsch positiven Komplement-Fixationsteste mit LED Seren und anti HBsAg Antiseren können demzufolge auf das Vorliegen von Anti-Antikörpern mit Gammaglobulinspezifität zurÜckgeführt werden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01471575
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  • 3
    Electronic Resource
    Electronic Resource
    Serum and cellular factor involvement in nitroblue tetrazolium (NBT) reduction by human neutrophils (1973)
    Springer
    Journal of molecular medicine 51 (1973), S. 377-382 
    Details
    ISSN: 1432-1440
    Keywords: Phagocytosis ; leukocytes ; infectious diseases ; tetrazolium salts ; Phagocyten ; leukocyten ; Infektionskrankheiten ; Tetrazolium-Salze
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird eine Modifikation des NBT-Testes beschrieben, welche es ermöglicht, serum- und celluläre Einflüsse auf die NBT-Reduktion durch menschliche Leukocyten getrennt zu untersuchen. Zu diagnostischen Zwecken wurden die Leukocyten in Hanksscher Lösung mit oder ohne autologem Serum inkubiert. Als dritter Parameter diente die einfach durchzuführende Stimulation mit E. coli. Reproduzierbarkeit und Empfindlichkeit der Methode wurden mit verschiedenen Substanzen experimentell geprüft. Endotoxin und E. coli stimulieren die NBT-Reduktion dosisabhängig, Prednisolon und Cytochalasin üben einen Hemmeffekt aus. In der Anwendung dieses Drei-Komponenten-NBT-Testes auf ein definiertes Patientengut ergaben sich verschiedene Reaktionsmuster, denen stimulierende oder hemmende Serumund/oder Zell-Faktoren zugrunde liegen.
    Notes: Summary The nitroblue tetrazolium (NBT) test has been modified in an attempt to separately assess leukocytic and serum factors in the reduction of NBT. For diagnostic purposes, NBT processing is assayed on leukocytes suspended in Hanks' solution in the absence and in the presence of serum, as well as with E. coli stimulation. The method has been tested for its reproducibility and for the suspceptibility of the leukocytes to stimulatory and inhibitory agents. Endotoxin and E. coli produce a dose-dependent stimulation, whereas prednisolone and cytochalasin B cause a dose-dependent inhibition of NBT processing. Applied to patients, different reaction patterns have been observed, revealing stimulatory and inhibitory serum factors, as well as increased and decreased cellular reactivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01468085
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  • 4
    Electronic Resource
    Electronic Resource
    High doses of antigen-nonspecific IgG do not inhibit pemphigus acantholysis in skin organ cultures (1985)
    Springer
    Archives of dermatological research 277 (1985), S. 299-303 
    Details
    ISSN: 1432-069X
    Keywords: Polyclonal, polyspecific human IgG ; Pemphigus ; Skin organ culture ; Plasma exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A patient suffering from severe pemphigus vulgaris was treated using large-volume plasma exchange in combination with an immunosuppressive regimen. As some recent reports have shown evidence that polyclonal, polyspecific human IgG in high doses through the i.v. route (IGIV) protect target platelets in idiopathic thrombocytopenic purpura from attack by antiplatelet autoantibodies and/or immune complexes, we also administered IGIV to this pemphigus-vulgaris patient. In order to test the hypothesis that IGIV might protect in vitro-cultured human skin from acantholysis induced by pemphigus antibodies, studies with skin organ cultures were carried out using plasma from another pemphigus-vulgaris patient who had undergone plasma exchange. The preincubation of either the skin explants or the pemphigus plasma with various concentrations of IGIV (ranging from 0.15 to 15 mg/ml in the culture medium) did not prevent acantholysis induced by the pemphigus plasma nor did it inhibit the binding of the specific antibodies visualized by direct immuno-fluorescence. Thus, the assumption that IGIV may coat the pemphigus antigens on epidermal cells making them inaccessible to pathogenic autoantibodies was not substantiated by our tests in vitro; likewise, the hypothesis of functionally blocking autoantibody activity by means of anti-idiotype effects of IGIV cannot be supported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00509084
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  • 5
    Electronic Resource
    Electronic Resource
    An Extended Clq-Binding Assay Using Lactoperoxidase- and Chloramine-T-Iodinated Clq (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 18 (1983), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: An extension of the Clq-binding assay for the detection of immune-aggregate-mediated and non-immune-aggregate-mediated Clq binding is reported. The assay involves the use of two different Clq preparations, one radioiodinated by means of lactoperoxidase (LPO-125I-Clq) and the other by means of chloramine-T (CT-125I-Clq). The treatment with CT for 20 min at room temperature before iodination for 1 min led to abolishment of the Clq-binding capacities to complexed IgG: approximately 50% of LPO-125I-Clq but only 2% of CT-125I-Clq bound to 80 μg/ml of IgG forming part of tetanus toxoid/anti-tetanus toxoid complexes or to 200 μg/ml of heat-aggregated human gamma globulin. Similar results were obtained with staphylococcal protein-A-aggregated IgG. CT-treated Clq was haemolytically inactive. In contrast to the results with complexed IgG, CT treatment did not markedly reduce binding capacities of Clq to heparin: approximately 55% of LPO- and CT-125I-Clq were bound by 127 U/ml of commercial heparin in normal human serum. Both Clq preparations bound to a comparable extent to fibronectin, fibrinogen, and various bacterial endotoxins. When the LPO- and CT-125I-Clq-binding patterns obtained on serum samples from patients with systemic lupus erythematosus, rheumatoid arthritis, or essential mixed cryoglobulinaemia were compared with binding patterns observed using laboratory reactants, an immediate detection of non-immune-aggregate-mediated Clq binding became possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1983.tb01803.x
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  • 6
    Electronic Resource
    Electronic Resource
    Polyvalent immunoglobulins for prophylaxis of bacterial infections in patients following multiple trauma (1985)
    Springer
    Intensive care medicine 11 (1985), S. 288-294 
    Details
    ISSN: 1432-1238
    Keywords: Immunoglobulins ; Polytrauma ; Head injury ; Mechanical ventilation ; Nosocomial infections
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract One hundred and fifty severely injured patients requiring long-term artificial ventilation were evaluated in a prospective, randomized, double blind study comparing the prophylactic effect of an intravenous immunoglobulin (Sandoglobulin; IGIV) against nosocomial infections with a placebo preparation. The groups were comparable in age, sex, injury pattern, and severity of the trauma. Seventy-six patients received 12 g of Sandoglobulin as a 3% solution on day 0, day 5 and day 12, i.e. a total of 36g. Sandoglobulin significantly reduced the incidence of pneumonia (28 cases in the IGIV group, 43 cases in the placebo group, p=0.0111). This resulted in a reduced therapeutic use of antibiotics. For the occurrence of sepsis (IGIV: 14 cases; placebo 19 cases) and other infections (IGIV: 11 cases; placebo: 10 cases) no significant differences were found. No side effects of the administration of IGIV were observed. IGIV prophylaxis neither reduced the overall death rate nor those deaths caused by infection. On day 5 after administration of the first 12 g of IGIV, the IgG serum concentrations were significantly higher in the Sandoglobulin group (8.41±1.96 mg/ml and 7.42±2.25 mg/ml respectively, p〉0.001) whereas later serum samples showed no significant differences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273538
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  • 7
    Electronic Resource
    Electronic Resource
    Comparison of posttransfusion recoveries achieved with either fresh or stored platelet concentrates (1987)
    Springer
    Annals of hematology 54 (1987), S. 207-212 
    Details
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effectiveness of platelet concentrate transfusion depends on such variables as blood bag material, donor — recipient compatibility, and time elapsed between donation and transfusion. To study the latter a corrected thrombocyte increment for recovery in the recipients was evaluated with 108 platelet transfusions in 31 patients. In 83 treatment programs, the mean recovery at the one-hour post-transfusion time point was 8.6×109 platelets/l with fresh platelets and 5.9×109 platelets/l with stored platelets. Significantly better recovery was achieved with freshly prepared platelet over the total of platelet concentrates stored for up to 96 hours; however, if the recoveries in different patient groups given stored platelets were considered separately in terms of storage times of up to 48 h or 48–96 h, the good recovery with fresh platelets was significantly better only when compared to the older (p=0.034) but not to the younger group of stored platelets. In patients with signs indicating enhanced platelet destruction (fever, splenomegaly, disseminated intravascular coagulation) the transfusion with fresh platelet concentrates gave a significantly better recovery compared to stored platelet concentrates (p=0.028), whereas in the absence of such signs the recovery produced by fresh concentrates was not significantly higher than with stored concentrates. These findings may be relevant for the logistics in blood banking.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00594195
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