ISSN:
1432-203X
Keywords:
Key words Eastern gamagrass
;
Tripsacum
;
Chromosome doubling
;
Tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Eastern gamagrass, (Tripsacum dactyloides L.) is a perennial, warm-season grass that is being developed as a forage plant. Shoots were derived from callus initiated from immature embryos and immature inflorescences of diploid (2n=2x=36) gynomonoecious eastern gamagrass. These shoots were induced to microtiller in the presence of 3 mg/l benzyladenine. Amiprophosmethyl (10, 15, or 20 μm) was applied to 27 microtillers for 3–5 days to induce chromosome doubling. All 14 surviving plants were tetraploid, (2n=4x=72), as determined by flow cytometry or chromosome counts. These plants were morphologically normal and produced seed. Test crosses were made with a known diploid. Flow cytometry and chromosome counts showed that the progeny were triploid, proving that the induced tetraploids reproduce sexually.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050502
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