ISSN:
1615-2573
Keywords:
Key words Diadenosine tetraphosphate
;
Cardioplegia
;
KATP channel
;
Purine receptor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Preischemic administration of diadenosine tetraphosphate (AP4A) has been shown to be cardioprotective. We evaluated the protective effect of AP4A when used as a cardioplegic adjuvant and tested contributions of the ATP-sensitive potassium channel (KATP channel), adenosine receptor (AR), and purine 2y receptor (P2yR) to the effect of AP4A. Isolated buffer-perfused rat hearts were subjected to 23 min of ischemia (37°C) followed by 20 min of reperfusion. Cardioplegia solution (St. Thomas Hospital solution) was infused during the first 3 min of ischemia. AP4A (10 μM) or AP4A with glibenclamide (KATP channel blocker, 100 μM), 8-SPT (AR antagonist, 300 μM) or reactive blue (P2yR antagonist, 13 nM) were added to the cardioplegia solution. Compared with the cardioplegia solution alone, administration of AP4A with the solution significantly increased the recovery of rate-pressure production (75% ± 11% vs 58% ± 10%; P 〈 0.05) and dp/dt at the end of reperfusion, and reduced the leakage of creatine kinase (3.2 ± 3.7 vs 13.2 ± 10.1 IU/g; P 〈 0.05) during reperfusion. This effect was reversed by coadministration of glibenclamide or reactive blue but not 8-SPT. The addition of AP4A into the cardioplegia solution led to an added cardioprotective effect, either by opening the KATP channel or by activating P2yR.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s003800070045
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