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Expression of augmenter of liver regeneration (ALR) in human liver cirrhosis and carcinoma (2005)

Thasler, W E ; Schlott, T ; Thelen, P ; [et al.]

Oxford, UK : Blackwell Science Ltd

Histopathology 47 (2005), S. 0

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ISSN: 1365-2559

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Aims : To determine the expression of a protein termed augmenter of liver regeneration (ALR), recently found to have a specific and beneficial effect on the process of liver regeneration in normal and diseased human liver.Methods and results : ALR expression in normal and cirrhotic human livers with various underlying diseases as well as in tissue samples of hepatocellular carcinoma (HCC) and cholangiocellular carcinoma (CCC) was analysed by immunohistochemistry and quantitative reverse transciptase-polymerase chain reaction (RT-PCR). Expression analysis of ALR in total liver protein extracts by Western blotting showed mainly dimeric ALR protein. Immunohistochemically, cytosolic and perinuclear immunosignals were found in hepatocytes and cholangiocytes in normal, cirrhotic or cancerous liver tissue and only weak signals in some endothelial cells in normal livers. Quantitative mRNA analysis revealed significantly increased ALR expression in cirrhosis compared with normal liver tissue. In HCC and CCC ALR mRNA expression was also significantly enhanced compared with normal liver tissue, but expression levels did not differ from the matching non-neoplastic tissue in the same patient.Conclusions : The findings suggest an important role for ALR in hepatocellular regeneration in liver cirrhosis as well as in hepatocarcinogenesis and therefore its potential value in the clinical diagnosis of hepatic cirrhosis and cancer.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2559.2005.02172.x

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Molecular characterization of the p83/100 proteins of variousBorrelia burgdorferi sensu lato strains (1996)

Rößler, D. ; Eiffert, H. ; Jauris-Heipke, S. ; [et al.]

Springer

Cellular and molecular life sciences 52 (1996), S. 305-306

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ISSN: 1420-9071

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Conclusions As in OspA-serotyping experiments, theB garinii group (OspA-sterotype 3–7) showed highest diversity within this internal fragment of p83/100, whereas theB. afzelii group (OspA-type 2) and theB. burgdorferi sensu stricto group (OspA-type 1) were nearly identical. Determination of the size of the PCR products as well as restriction fragment length polymorphism analysis (DraI) can be used for classification into the three species ofB. burgdorferi sensu lato. Since p83/100 is chromosomally encoded, this protein might be a more stable marker for classification than the plasmid-encoded OspA. In contrast to the flagellin gene a subclassification of theB. garinii group is possible due to the diversity of the p83/100 internal fragment.