ISSN:
1399-3054
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Biologie
Notizen:
The amino acid nicotianamine (NA) is essential for micronutrient metabolism in plants. Lack of NA results in a chlorotic phenotype and oxidative stress, since NA is a chelator of iron and other metal nutrients. To investigate the precise cellular function of NA in micronutrient transport and homeostasis, a protocol for the production of [14C]-labelled NA was developed. Recombinant NA synthase was used to generate [14C]-NA from [14C]-S-adenosylmethionine. After purification by solid-phase ion exchange about 66% yield was achieved. The identity of the [14C]-NA with chemically synthesized NA was demonstrated by several independent methods, including two TLC systems, two HPLC systems and immuno-detection. Moreover, biological function was shown by complementation of the Lycopersicon esculentum mutant chloronerva that is free of NA due to a defect in NA synthase. Proof-of-function for the produced [14C]-NA as a suitable tool for transport studies was provided monitoring the distribution of [14C]-NA after feeding to tomato and Ricinus communis seedlings.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1034/j.1399-3054.2003.00128.x
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