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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of a gene essential for biosynthesis of the lipopeptide antibiotics plipastatin B1 and surfactin in Bacillus subtilis YB8 (1996)
    Springer
    Archives of microbiology 165 (1996), S. 243-251 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsBacillus subtilis ; plipastatin B1 ; Surfactin ; Lipopeptide antibiotics ; Nucleotide sequence of lpa-8
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bacillus subtilis YB8 was found to produce the lipopeptide antibiotics surfactin and plipastatin B1. A gene, lpa-8, required for the production of both lipopeptides was cloned from strain YB8. When this gene was inactivated in strain YB8, neither surfactin nor plipastatin B1 was produced. However, the defective strain transformed with an intact lpa-8 gene had restored ability to produce both peptides. Nucleotide sequence analysis of the region essential for the production of the peptides revealed the presence of a large open reading frame. The deduced amino acid sequence of lpa-8 (224 amino acid residues) showed sequence similarity to that of sfp (from surfactin-producing B. subtilis), lpa-14 (from iturin A- and surfactin-producing B. subtilis), psf-1 (from surfactin-producing Bacillus pumilus), gsp (from gramicidin-S-producing Bacillus brevis), and entD (from siderophore-enterobactin-producing Escherichia coli), which are able to complement a defect in the sfp gene and promote production of the lipopeptide antibiotic surfactin. The sequence similarity among these proteins and the product similarity of cyclic peptides suggests that they might be involved in the biosynthesis or secretion of the peptides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050322
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  • 2
    Electronic Resource
    Electronic Resource
    Plasmid transformation in Bacillus subtilis NB22, an antifungal-antibiotic iturin producer (1990)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 67 (1990), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A transformation system with plasmids was developed for Bacillus subtilis NB22, an antibiotic iturin producing strain. Treatment of B. subtilis NB22 with 4 M KCl was effective for the induction of competence, followed by uptake of plasmid DNA in the presence of polyethylene glycol. The efficiency of transformation of this bacterium with pC194 and pUB110 was 4.1 × 103 and 1.5 × 103 transformants per μg DNA, respectively and the transformation frequency was 3.3 × 10−3 and 7.2 × 10−4, transformants per viable cell, respectively. This method was much faster and three orders of magnitude more efficient in transformation efficiency than protoplast transformation methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1990.tb13868.x
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  • 3
    Electronic Resource
    Electronic Resource
    Transformation ofBacillus subtilis with the treatment by alkali cations (1990)
    Springer
    Biotechnology letters 12 (1990), S. 99-104 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary ExposingBacillus subtilis cultures to high concentrations of alkali cations, especially K+, allows efficient transformation by plasmids. The method allows transformation with unfractionated plasmid DNA, monomeric plasmid DNA as well as linear plasmid DNA.B. subtilis strains, not amenable to natural transformation, were also transformed by the present method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01022423
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  • 4
    Electronic Resource
    Electronic Resource
    A newly isolated heterotrophic bacterium,Xanthomonas sp. DY44, to oxidize hydrogen sulfide to polysulfide (1991)
    Springer
    Biotechnology letters 13 (1991), S. 923-928 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A newly isolated heterotrophic bacterium,Xanthomonas sp. DY44, was found to be capable of oxidizing hydrogen sulfide (H2S). Cells made non-viable by heat treatment (120°C, 20 min) did not show H2S oxidation. However, both cells sterilized by γ-rays irradiation and cell-free extract oxidized H2S, suggesting the existence of the heat-labile intracellular enzymatic system for H2S oxidation. AsXanthomonas sp. DY44 exhibited no autotrophic growth with H2S in basal mineral medium, the H2S oxidation was judged not to be a consequence of chemolithotrophic activity. Using X-rays photoelectron spectroscopy (XPS), the metabolic product of H2S oxidation was assessed to be polysulfide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01022100
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  • 5
    Electronic Resource
    Electronic Resource
    Production of a lipopeptide antibiotic surfactin with recombinantBacillus subtilis (1992)
    Springer
    Biotechnology letters 14 (1992), S. 1165-1168 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Production of a lipopeptide antibiotic surfactin was carried out using a recombinantBacillus subtilis. Surfactin yield of the recombinant strain was about one and half times as much as that ofBacillus subtilis RB 14, the strain in which the surfactin gene was originated. This system is especially noteworthy because a recombinant strain surpassed the original strain in the production of a bacterial antibiotic as a secondary metabolite of the bacterium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01027022
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of super high magnetic field on lysogenic lambda phage with a temperature sensitive repressor (1992)
    Springer
    Biotechnology letters 14 (1992), S. 765-768 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Escherichia coli where bacteriophage λ was lysogenic was grown under the super high magnetic field (11.7 Tesla) and the effect of the field on the transition from lysogenic to lytic process of the phase was investigated. The occurrence of phage particles due to induction of phage was stimulated under 11.7 T in comparison with that in geomagnetic field by raising temperature from 30 to 45°C. Especially at 35°C, the phase titer was tenfold larger. No significant effect of the field on the phase particleper se was observed. A potential application of high magnetic strength as a controlling factor ofin vivo switching was implied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01029135
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  • 7
    Electronic Resource
    Electronic Resource
    Decolorization of molasses by a new isolate of Geotrichum candidum in a jar fermenter (1998)
    Springer
    Biotechnology techniques 12 (1998), S. 497-499 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Decolorization of crude molasses by a newly isolated dye-decolorizing fungus, Geotrichum candidum Dec 1, was conducted in a jar fermentor system having fan-type propellers and a pressure swing adsorption oxygen generator. The oxygen-fortified air supply was effective not only to obtain a highest decolorization degree of 80% molasses but also the highest peroxidase activity which is responsible for the decolorization of the dyes. © Rapid Science Ltd. 1998
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008824119174
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  • 8
    Electronic Resource
    Electronic Resource
    Co-utilization of Bacillus subtilis and Flutolanil in controlling damping-off of tomato caused by Rhizoctonia solani (2000)
    Springer
    Biotechnology letters 22 (2000), S. 1693-1697 
    Details
    ISSN: 1573-6776
    Keywords: Bacillus subtilis ; Flutolanil ; integration ; iturin A ; Rhizoctonia solani
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Damping-off of tomato caused by Rhizoctonia solani was controlled in a pot test using the biological agent, Bacillus subtilis RB14-C, and the chemical pesticide, Flutolanil. The co-utilization of B. subtilis RB14-C, and Flutolanil decreased the amount of Flutolanil used from 375 μg/pot when Flutolanil was used alone to 94 μg/pot, while exerting the same effect of reducing disease occurrence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005675829479
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  • 9
    Electronic Resource
    Electronic Resource
    Cultivation of baker's yeast by a fermenter with a basket-shaped unit for agitation (1989)
    Springer
    Biotechnology techniques 3 (1989), S. 381-384 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A simple and new basket-shaped unit for agitation made of stainless wire was developed. A fermenter equipped with this unit attained higher kLa, than 3500 h−1 under the condition with an aeration rate 2vvm and a rotation speed of the unit 1100rpm. In the cultivation of baker's yeast, the cell concentration reached about 110g per liter on dry weight basis in 17.5h.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01875003
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  • 10
    Electronic Resource
    Electronic Resource
    Isolation of electric pulse-induced Escherichia coli genes by operon fusion techniques (1993)
    Springer
    Biotechnology techniques 7 (1993), S. 719-722 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A search of electric-pulse induced genes in Escherichia coli was carried out by operon fusion techniques with a hybrid bacteriophage Mu, which creates transcriptional fusions of the structural gene of β-galactosidase to the host. Among two hundred transductants tested, nine colonies showed higher expression of more than twofold when they were treated with electric pulses. An electric-pulse stimulated transductant was not stimulated by UV irradiation, which is known to induce an SOS response. Conversely, strain PQ37, which has an operon fusion in one of the SOS genes, did not respond to an electric pulse treatment. A possibility of the presence of the electric-pulse stimulated genes, which were not induced by DNA damages, was suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00152619
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