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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Water and environment journal 10 (1996), S. 0 
    ISSN: 1747-6593
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Recent work in the US has shown that contact between lubricants and oils and drinking water can promote the growth of heterotrophic micro-organisms. It is unlikely that these species are pathogenic, and therefore harmful to man. However, the hygienic status of water distribution systems could be affected, leading to higher heterotrophic plate counts during routine sampling. Experimental work has been undertaken to study the microbiological impact of a range of lubricants and oils on drinking water. The methods have been based on an existing procedure for the assessment of the growth potential of drinking water, i.e. the ‘assimilable organic carbon’test. The results of this work could serve as the basis of a future standard method for the rapid and inexpensive screening of oils and lubricants for their microbiological suitability in potable-water applications.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Water and environment journal 9 (1995), S. 0 
    ISSN: 1747-6593
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: The microbiological safety of water supplies is at present assured by monitoring for the absence of the faecal indicator organisms, total coliform bacteria and Escherichia coli. This monitoring work represents the bulk of the workload of microbiology laboratories in the water industry. The accuracy of current methods is not questioned, but the times taken to obtain a presumptive result (18 h) and a confirmed result (up to 72 h) impose delays, particularly with regard to the recommissioning of distribution systems. There is therefore a continued demand for more rapid techniques, which has not been satisfied, despite the advances in analytical methods which have been adopted in other industries. Alternatives to the traditional culture-based methods are reviewed in this paper, and promising techniques for water applications are highlighted. Highly specific bacterial labelling methods and sensitive instrumentation for detection and enumeration already exist, so that rapid same-day detection of coliforms is technically feasible. Suitable separation methods for rapid analysis of water samples have not yet been developed, but antibody-mediated separations which are now employed in clinical and food microbiology may be applicable.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0884-3996
    Keywords: Luminometer ; evaluation ; rapid microbiology ; ATP assay ; firefly luciferase ; photometer ; radiometer ; comparison ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: An assessment has been carried out of the relative performance of ten instruments for quantification of adenosine triphosphate (ATP) by the firefly luciferase assay. The instruments evaluated were Amersham Amerlite Analyser, Dynatech Tube Luminometer, Dynatech Multiplate Luminometer, Dynatech Camera Luminometer, Hamilton Lumicon, LKB 1250 Luminometer, LKB 1251 Luminometer, Lumac Biocounter M2010A, Turner 20 TD Luminometer and a prototype version of the CLEAR Speed Tech 2000. An 800-fold difference in sensitivity was found between the most sensitive (Lumac, Turner) and the least sensitive (Dynatech Tube) of the conventional instruments. The Dynatech Camera Luminometer which worked on a completely different principle to the other instruments was about 5000 times less sensitive than the best of the photomultiplier tube instruments. The relative sensitivity of the instruments was maintained regardless of whether solutions of ATP in water or trichloroacetic acid extracts of bacteria were analysed. An analysis of 960 ATP bioluminescence assays showed that data obtained from such measurements are normally distributed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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