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  • 1
    Electronic Resource
    Electronic Resource
    A proposed framework for the description of plant metabolomics experiments and their results (2004)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 1601-1606 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The study of the metabolite complement of biological samples, known as metabolomics, is creating large amounts of data, and support for handling these data sets is required to facilitate meaningful analyses that will answer biological questions. We present a data model for plant metabolomics known ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1041
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  • 2
    Electronic Resource
    Electronic Resource
    Sterols of male and female compound inflorescences of Zea mays L. (1976)
    Springer
    Planta 133 (1976), S. 89-93 
    Details
    ISSN: 1432-2048
    Keywords: Zea mays ; Inflorescences ; Male ; Female ; Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comparison has been made between the sterols of male and female inflorescences and of pollen from Zea mays. The female inflorescence was shown to contain cholesterol, 24-methylcholesterol, 24-ethyl-5,22-cholestadien-3β-ol, 24-ethylcholesterol and (28Z)-24-ethylidenecholesterol. Themale inflorescence contained the same five compounds together with 24-methylenecholesterol. Pollen contained 24-methylenecholesterol as its main sterol together with lesser amounts of cholesterol, 24-ethylcholesterol, (28Z)-24-ethylidenecholesterol, 24-methylene-5α-cholest-7-en-3β-ol and 4α-methyl-24-methylene-5α-cholest-7-en-3β-ol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386011
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  • 3
    Electronic Resource
    Electronic Resource
    Sterols of male and female flowers of Cucumis sativus (1977)
    Springer
    Planta 134 (1977), S. 115-117 
    Details
    ISSN: 1432-2048
    Keywords: Female ; Flowers ; Male ; Meiosis ; Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sterols of male and female flowers of Cucumis sativus L. were similar in composition. The principal compound was 24ξ-ethyl-5α-cholesta-7,22-dien-3β-ol. Five other 5α-Δ7 were detected: 24ξ-methyl-7-ene, 24ξ-ethyl-7-ene, 24-ethyl-7,24(28)Z-diene, 24ξ-ethyl-7,25-diene and 24ξ-ethyl-7,22,25-triene. Small amounts of Δ5 (cholesterol, 24ξ-methylcholesterol and 24ξ-ethylcholesterol) were detected. The possible significance of these sterols is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384959
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  • 4
    Electronic Resource
    Electronic Resource
    The effect of water stress on 1-(malonylamino)cyclopropane-1-carboxylic acid concentration in plant tissues (2000)
    Springer
    Plant growth regulation 31 (2000), S. 183-193 
    Details
    ISSN: 1573-5087
    Keywords: gas chromatography ; mass spectrometry ; 1-aminocyclopropane-1-carboxylic acid ; 1-(malonylamino)cyclopropane-1-carboxylic acid ; Lycopersicon esculentum Mill.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Since the discovery of1-(malonylamino)cyclopropane-1-carboxylic acid (MACC)as a major metabolite of both endogenous andexogenously applied 1-aminocyclopropane-1-carboxylicacid (ACC), it has become evident that the formationof MACC from ACC can act to regulate ethyleneproduction in certain tissues. Hence it was suggestedthat MACC could serve as an indicator of water-stresshistory in plant tissues. The accurate quantificationof MACC in plant tissues is essential forunderstanding the role of MACC in the regulation ofethylene biosynthesis.Hoffman et al. [15] described a method for themeasurement of MACC in which MACC was hydrolysed byHCl to ACC, which was then assayed by chemicaloxidation to form ethylene. Attempts have been made byothers to raise monoclonal antibodies to MACC so thatan immunoassay could be developed in order to gain adeeper understanding of stress-induced ethyleneproduction but no further publications have beenforthcoming.Here a method employing GC-MS is compared with theindirect assay for MACC, which is based uponhydrolysis of MACC to ACC and conversion of ACC byhypochlorite reagent to ethylene which is subsequentlyquantified by GC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006354415329
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  • 5
    Electronic Resource
    Electronic Resource
    The quantification of 1-(malonylamino)cyclopropane-1-carboxylic acid in plant tissues by quantitative mass spectrometry (1996)
    Springer
    Plant growth regulation 19 (1996), S. 133-137 
    Details
    ISSN: 1573-5087
    Keywords: gas chromatography ; mass spectrometry ; 1-(malonylamino)cyclopropane-1-carboxylic acid ; Triticum aestivum L. ; Lycopersicon esculentum Mill
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A method for the quantitation of 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC), a conjugated form of 1-aminocyclopropane-1-carboxylic acid (ACC), in plants is described. [2,2,3,3-2H4]MACC has been used as an internal standard for selected ion monitoring/isotope dilution quantitation of MACC in wheat seedlings and in tomato leaves. This method is compared with a widely-used two step indirect assay for MACC, which is based upon hydrolysis of MACC to ACC and conversion of ACC by hypochlorite reagent to ethylene which is subsequently quantified by gas chromatography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024579
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  • 6
    Electronic Resource
    Electronic Resource
    Explanatory Analysis of the Metabolome Using Genetic Programming of Simple, Interpretable Rules (2000)
    Springer
    Genetic programming and evolvable machines 1 (2000), S. 243-258 
    Details
    ISSN: 1573-7632
    Keywords: metabolome ; tomato fruit ; salinity ; Fourier transform infra-spectroscopy (FTIR) ; chemometrics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract Genetic programming, in conjunction with advanced analytical instruments, is a novel tool for the investigation of complex biological systems at the whole-tissue level. In this study, samples from tomato fruit grown hydroponically under both high- and low-salt conditions were analysed using Fourier-transform infrared spectroscopy (FTIR), with the aim of identifying spectral and biochemical features linked to salinity in the growth environment. FTIR spectra of whole tissue extracts are not amenable to direct visual analysis, so numerical modelling methods were used to generate models capable of classifying the samples based on their spectral characteristics. Genetic programming (GP) provided models with a better prediction accuracy to the conventional data modelling methods used, whilst being much easier to interpret in terms of the variables used. Examination of the GP-derived models showed that there were a small number of spectral regions that were consistently being used. In particular, the spectral region containing absorbances potentially due to a cyanide/nitrile functional group was identified as discriminatory. The explanatory power of the GP models enabled a chemical interpretation of the biochemical differences to be proposed. The combination of FTIR and GP is therefore a powerful and novel analytical tool that, in this study, improves our understanding of the biochemistry of salt tolerance in tomato plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1010014314078
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