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  • 1
    Electronic Resource
    Electronic Resource
    Studies on a new proteolytic enzyme from Achromobacter lyticus M497-1 I. Purification and some enzymatic properties
    Amsterdam : Elsevier
    BBA - Enzymology 660 (1981), S. 44-50 
    Details
    ISSN: 0005-2744
    Keywords: (Achromobacter lyticus) ; Amino acid composition ; Protease purification
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2744(81)90106-6
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on a new proteolytic enzyme from Achromobacter lyticus M497-1 II. Specificity and inhibition studies of Achromobacter protease I
    Amsterdam : Elsevier
    BBA - Enzymology 660 (1981), S. 51-55 
    Details
    ISSN: 0005-2744
    Keywords: (Achromobacter lyticus) ; Inhibition study ; Protease specificity
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2744(81)90107-8
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Augmented railgun using a permanent magnet (1995)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 66 (1995), S. 4227-4232 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The use of a permanent magnet instead of an electromagnet has been proposed for the augmentation of the magnetic field of a railgun driven by a current of approximately 20 kA. A permanent magnet has the following advantages in comparison with conventional augmentations using additional turns: (1) simple configuration of the system, (2) temporally and spatially constant magnetic fields, and (3) high efficiency. Here, the operation of a conventional railgun and that of an augmented railgun using a permanent magnet are compared experimentally, and the usefulness of the permanent magnet is described. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1145374
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Single anion-selective channel and its ion selectivity in the vascular smooth muscle cell (1988)
    Springer
    Pflügers Archiv 411 (1988), S. 304-311 
    Details
    ISSN: 1432-2013
    Keywords: Anion-selective Cl channel ; Cultured smooth muscle cell ; Ion selectivity ; Voltage-dependent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The properties of voltage-gated Cl channels of cultured smooth muscle cell prepared from embryonic rat aorta were studied. In the excised patch (inside-out configuration), we observed the activity of channels, opening and closing spontaneously, when the membrane potential was held at around 0 mV. The channels were active at a potential range between +10 and −10 mV. A step change of the membrane potential from the active potential range in either a positive or a negative direction closed the channel to an apparently inactivated state. The time course of this inactivation process became faster as the amplitude of the step change was increased. Returning the membrane potential to 0 mV allowed the channel to recover from the inactivated state. The channel had at least two open conductance states. Ca ions at the cytoplasmic face were not required for the activation of the channel. Adenosine nucleotides at the same side of the membrane had no effect on channel activity. The channels were selective to anions rather than cations, and they had a large single channel conductance of 340.5±20.4 pS in symmetrical 150 mM TEA-Cl. The reversal potential of the channel was shifted by −15.2±2.6 and 17.0±1.7 mV, when the Cl concentration at the intracellular side was changed to 75 mM or 300 mM, respectively. The permeability sequence of halides was I−〉Br−〉Cl−〉F− (1.4∶1.3∶1.0∶0.7), whereas the conductance sequence was Cl−〉Br−〉F−〉I− (1.00∶0.89∶0.86∶0.83). The internal dimension of the channel was estimated by measuring the permeability of various anions with different molecular cross section. We suggest that the smallest cross section of the channel pore is about 32Å2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585119
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  • 5
    Electronic Resource
    Electronic Resource
    An Alu-mediated large deletion of the FUT2 gene in individuals with the ABO-Bombay phenotype (2000)
    Springer
    Human genetics 〈Berlin〉 106 (2000), S. 80-85 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Recently, we have found an allelic deletion of the secretor α(1,2)fucosyltransferase (FUT2) gene in individuals with the classical Bombay phenotype of the ABO system. The FUT2 gene consists of two exons separated by an intron that spans approximately 7 kb. The first exon is noncoding, whereas exon 2 contains the complete coding sequence. Since the 5' breakpoint of the deletion has previously been mapped to the single intron of FUT2, we have cloned the junction region of the deletion in a Bombay individual by cassette-mediated polymerase chain reaction. In addition, the region from the 3' untranslated region of FUT2 to the 3' breakpoint sequence has been amplified from a control individual. DNA sequence analysis of this region indicates that the 5' breakpoint is within a free left Alu monomer (FLAM-C) sequence that lies 1.3 kb downstream of exon 1, and that the 3' breakpoint is within a complete Alu element (AluSx) that is positioned 1.5 kb downstream of exon 2. The size of the deletion is estimated to be about 10 kb. There is a 25-bp sequence identity between the reference DNA sequences surrounding the 5' and 3' breakpoints. This demonstrates that an Alu-mediated large gene deletion generated by unequal crossover is responsible for secretor α(1,2)fucosyltransferase deficiency in Indian Bombay individuals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004399900212
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Detection of T to G mutation at position 59 in the Lewis gene by mismatch polymerase chain reaction (1995)
    Springer
    International journal of legal medicine 107 (1995), S. 280-282 
    Details
    ISSN: 1437-1596
    Keywords: Blood group ; Lewis gene ; Mismatch PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract Recently, we discovered the missense mutations of T to G at position 59 and of G to A at position 508 in one of the Lewis-negative (le) genes (Koda et al. 1993). In the present study, we report a method to detect the mutation at position 59 using mismatch PCR amplification and endonucleaseMspI digestion. For this mutation, we found that 7 out of 12 Lewis-negative, and none of 15 Lewis-positive individuals were homozygous, while 4 out of 12 Lewis-negative, and 4 out of 15 Lewis-positive individuals were heterozygous. These results indicate that the mutation at position 59 is a common mutation in thele genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01246873
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