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  • 1
    Electronic Resource
    Electronic Resource
    Export of the periplasmic NADP-containing glucose-fructose oxidoreductase of Zymomonas mobilis (1996)
    Springer
    Archives of microbiology 166 (1996), S. 32-41 
    Details
    ISSN: 1432-072X
    Keywords: Key words Glucose-fructose oxidoreductase ; Zymomonas mobilis ; Gfo-deficient mutant ; Sorbitol ; Protein export ; Signal sequence ; Periplasmic NADP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Glucose-fructose oxidoreductase (GFOR) of the gram-negative bacterium Zymomonas mobilis is a periplasmic enzyme with the tightly bound cofactor NADP. The preprotein carries an unusually long N-terminal signal sequence of 52 amino acid residues. A sorbitol-negative mutant strain (ACM3963) was found to be deficient in GFOR activity and was used for the expression of plasmid-borne copies of the wild-type gfo gene or of alleles encoding alterations in the signal sequence of the pre-GFOR protein. Z. mobilis cells with the wild-type gfo allele translocated pre-GFOR, at least partially, via the Sec pathway since CCCP (carboxylcyanide-m-chlorophenylhydrazone; uncoupler of proton motive force) or sodium azide (inhibitor of SecA) abolished the processing of GFOR. A gfo allele with the hydrophobic region of the signal sequence removed (residues 32–46; Δ32–46) led to a protein that was no longer processed, but showed full enzymatic activity (180 U/mg) and had the cofactor NADP firmly bound. A deletion in the n-region of the signal sequence (residues 2–20; Δ2–20) or exchange of the entire GFOR signal sequence with the signal sequence of gluconolactonase of Z. mobilis led to active and processed GFOR. Strain ACM3963 could not grow in the presence of high sugar concentrations (1 M sucrose) unless sorbitol was added. The presence of the plasmid-borne gfo wild-type allele or of the Δ2–20 deletion led to the restoration of growth on media with 1 M sucrose, whereas the presence of the Δ32–46 deletion led to a growth behavior similar to that of strain ACM3963, with no sorbitol formation from sucrose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050352
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  • 2
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray crystallographic analysis of recombinant transaldolase B from Eschericha coli (1996)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 192-193 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Recombinant transaldolase from Escherichia coli, an enzyme of the pentose phosphate pathway has been crystallized by the vapor-diffusion method using polyethylene glycol 6000 as precipitant. The crystals are orthorhombic, space group P212121 with cell dimensions a = 68.9, b = 91.3 and c = 130.5 Å and diffract to 2 Å resolution on a conventional X-ray source. The asymmetric unit very likely contains two subunits, corresponding to a packing density of 2.9 Å3 Da−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444995010365
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  • 3
    Electronic Resource
    Electronic Resource
    A specific amino acid repeat in squalene and oxidosqualene cyclases
    Amsterdam : Elsevier
    Trends in Biochemical Sciences 19 (1994), S. 157-158 
    Details
    ISSN: 0968-0004
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0968-0004(94)90276-3
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  • 4
    Electronic Resource
    Electronic Resource
    Genetics and genetic engineering ofZymomonas mobilis (1993)
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 17-24 
    Details
    ISSN: 1573-0972
    Keywords: Cloning vectors ; ethanol ; plasmids ; strain improvement ; Zymomonas genetics ; Zymomonas mobilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Present knowledge on the genetics of the ethanologenic anaerobeZymomonas mobilis includes background information on: size, restriction, and to some extent hybridization, analysis of indigenous plasmids; mutagenesis and isolation of a wide variety of auxotrophic, drug resistant and conditional mutants; construction of shuttle cloning vectors able to replicate and express inZ. mobilis; development of gene transfer systems based on conjugal mobilization of plasmids fromEscherichia coli donors toZ. mobilis; expression of heterologous genes inZ. mobilis; cloning and analysis of genes encoding enzymes of the Entner-Doudoroff pathway. Moreover, preliminary data on recombinational repair mechanisms and plasmid stability, which are now available, makeZ. mobilis an attractive model system for molecular genetic research and, furthermore, they contribute towards expansion of the substrate and product range of this industrial microorganism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00656509
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  • 5
    Electronic Resource
    Electronic Resource
    Die Geschwindigkeit des monomolekularen Zerfalls einfacher Gase (1929)
    Springer
    Naturwissenschaften 17 (1929), S. 997-997 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01505862
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  • 6
    Electronic Resource
    Electronic Resource
    Die Darstellung und Eigenschaften des Nitrylchlorids (1929)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 182 (1929), S. 139-144 
    Details
    ISSN: 0863-1786
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Es wird eine neue Methode zur Darstellung des Nitrylchloride NO2Cl1 angegeben und dieses erstmalig rein dargestellt.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19291820112
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  • 7
    Electronic Resource
    Electronic Resource
    Über die Existenz höherer Stickoxyde (1929)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 42 (1929), S. 697-700 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19290422603
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