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The use of lignocellulosic wastes for production of cellulase by Trichoderma reesei (1987)

Doppelbauer, R. ; Esterbauer, H. ; Steiner, W. ; [et al.]

Springer

Applied microbiology and biotechnology 26 (1987), S. 485-494

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ISSN: 1432-0614

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary The feasibility of cellulase production by Trichoderma reesei using inexpensive lignocellulosic material was examined. Sulfite pulp used as standard substrate yielded 3.7 IU/ml filter paper units (FPU) and 2.15 IU/ml β-glucosidase. The yield was 185 FPU per gram total carbohydrate (CH) in the fermentation medium. Steam treated wheat straw (2%) gave 1.9 FPU/ml, 0.83 IU/ml β-glucosidase and 151 FPU/g CH, whereas the spent fibres remaining after enzymatic hydrolysis of steamed wheat straw gave 2.4 FPU/ml, 1.55 IU/ml β-glucosidase and 147 FPU/g CH. A good substrate (3%) was also the combustible fraction of municipal waste (BRAM) treated with NaOH, which gave 2.5 FPU/ml, 0.86 IU/ml β-glucosidase and 130 FPU/g CH. A further increase in the final enzyme titer is obtainable by increasing the substrate concentration. In shake cultures 5% steamed wheat straw gave 3.8 FPU/ml and 1.95 IU/ml β-glucosidase. Untreated wheat straw gave only low final enzyme titers and low yields of FPU/g CH. In the case of lignocellulosic substrates a constant pH-value of pH 6.0 during the fermentation gave optimal yields.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00253537

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Conversion of lignocellulosic material to ethanol influence of raw material yield and hemicellulose utilization on sales price of ethanol (1988)

Felber, J. ; Schiefersteiner, M. ; Steinmüller, H.

Berlin : Wiley-Blackwell

Acta Biotechnologica 8 (1988), S. 299-303

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ISSN: 0138-4988

Keywords: Life Sciences ; Life Sciences (general)

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: In the late 70's an Austrian Consortium consisting of the STEYRERMÜHL-Papierfabriks- und Verlags AG, the Voest-Alpine AG and the Universities of Graz was formed to develop a new process for the production of monosaccharides from renewable carbohydrate sources, in particular lignocellulosic material. After detailed examination of various hydrolytic processes it was decided to intensify work on enzymatic hydrolysis. The composition of this group brought the great advantage that it could focus not only on one problem but could also research into the total process. This included pretreatment, enzyme production, hydrolysis, by-product utilization and energy supply.The raw materials studied most thoroughly on our program were waste paper and wheat straw, since these lignocellulosics are available in large quantities in Austria.Until now all endevour to produce ethanol out of lignocellulosic biomass on an industrial scale failed due to uneconomical production. Thus it is evident that the economy of such a process is very dependent not only on the yield of sugar, which is strongly affected by the respective pretreatment, but also on the utilization of the hemicellulose. Summarizing it can be said that the cellulose has to be degregated to a high extent and that the hemicellulose must be utilized to reach a feasible project.

Additional Material: 5 Ill.