ISSN:
1365-2761
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Abstract. The kinetics of gentamicin and tobramycin in juvenile brown sharks, Carcharhinus plumbeus (Nardo), were studied using a commercially available radioimmune assay (Clinical Assays, Cambridge, Massachusetts). Preliminary recovery studies in vitro demonstrated 95% recovery of antibiotic spiking with no detection of interfacing substances. There were no differences between serum and plasma data using this assay. Both drugs demonstrated distinct two-compartment characteristics. The mean half-life of the alpha component was 5.4±1.0 h for gentamicin and 3.2±0.5 h for tobramycin. The half-lives of the beta components were markedly longer, 54±4.9 h for gentamicin and 48±2 h for tobramycin. The experimental designs could not exclude third compartments. A therapeutic dosage schedule for gentamicin of 2 mg/kg intramuscularly followed by 1 mg/kg at 8 and 72 h was tested. This schedule avoided plasma levels greater than 12μg/ml and troughs greater than 2 μg/ml but more frequent administration may be required to treat infections with bacteria which are less sensitive to aminoglycoside antibiotics. A therapeutic tobramycin schedule of 2.5 mg/kg intramuscularly followed by 1.0 mg/kg, 4 h later and daily thereafter, achieved plasma levels within the human therapeutic range (5.8μg/ml) on the second day of therapy. Levels considered toxic to humans were not reached for 5 days.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2761.1986.tb01019.x
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