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  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 54 (1998), S. 281-283 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: PAPS reductase from E. coli is involved in sulfur metabolism and catalyses the reduction of phospho-adenylyl-sulfate (PAPS) to sulfite. The protein has been cloned, overexpressed and purified from E. coli. Crystallization experiments resulted in crystals suitable for X-ray diffraction. The crystals belong to the orthorhombic space group C2221 with cell dimensions a = 81.9, b = 97.4, c = 109.5 Å, and contain one molecule per asymmetric unit. At cryogenic (100 K) temperatures the crystals diffract to a resolution limit of 2.7 Å using a rotating anode and to 2.0 Å at a synchrotron source.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 385 (1997), S. 365-368 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The SRP-type GTPases form a distinct subfamily of GTPases10. The NG domain was identified by sequence alignments3'4 in all SRP-type GTPases, and could also be obtained as a stable proteolytic fragment from both FtsY (ref. 7) and SRP54 (ref. 12), the signal sequence-binding protein (Ffh in E. coli). ...
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1572-9699
    Keywords: carbon catabolite repression ; glucose kinase ; Streptomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Glucose kinase of Streptomyces coelicolor A3(2) is essential for glucose utilisation and is required for carbon catabolite repression (CCR) exerted through glucose and other carbon sources. The protein belongs to the ROK-family, which comprises bacterial sugar kinases and regulators. To better understand glucose kinase function, we have monitored the cellular activity and demonstrated that the choice of carbon sources did not significantly change the synthesis and activity of the enzyme. The DNA sequence of the Streptomyces lividans glucose kinase gene glkA was determined. The predicted gene product of 317 amino acids was found to be identical to S. coelicolor glucose kinase, suggesting a similar role for this protein in both organisms. A procedure was developed to produce pure histidine-tagged glucose kinase with a yield of approximately 10 mg/l culture. The protein was stable for several weeks and was used to raise polyclonal antibodies. Purified glucose kinase was used to explore protein-protein interaction by surface plasmon resonance. The experiments revealed the existence of a binding activity present in S. coelicolor cell extracts. This indicated that glucose kinase may interact with (an)other factor(s), most likely of protein nature. A possible cross-talk with proteins of the phosphotransferase system, which are involved in carbon catabolite repression in other bacteria, was investigated.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 28 (1997), S. 285-288 
    ISSN: 0887-3585
    Keywords: SRP ; SRP receptor ; GTPase ; expression ; crystallization ; x-ray diffraction ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: FtsY is the docking protein or SRα homologue in E. coli. It is involved in targeting secretory proteins to the cytoplasmic membrane by interacting with the signal recognition particle, controlled by guanosine 5′-triphosphate. Two different constructs have been used in crystallization studies: the full-length protein and a truncated fragment with a his-tag at the C terminus. Only the second construct resulted in crystals suitable for x-ray diffraction. The crystals belong to the monoclinic space group P21 with cell dimensions a = 32.20 Å, b = 79.57 Å, c = 59.21 Å, and β = 94.45, and contain one molecule per asymmetric unit. At cryogenic temperatures the crystals diffract to a resolution limit of 2.5 Å by using a rotating anode, and beyond 1.8 Å by using synchrotron radiation. Proteins 28:285-288, 1997. © 1997 Wiley-Liss Inc.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied climatology 59 (1998), S. 79-91 
    ISSN: 1434-4483
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Summary During the summer season, typhoons form in the western north Pacific Ocean and travel westward towards China. Some recurve northward off the coast, whereas others continue in over land. These typhoons bring heavy rainfall to the Huai river basin in eastern central China. In August 1975, the remnant of typhoon Nina caused exceptionally heavy rainfall in the Hongru river basin, in the mountainous upper reaches of the Huai river. The rainfall lasted five days from 4 to 8 August. This type of nearly stationary typhoon can cause rainfall of large intensity for a long duration, and is suitable for maximization to derive probable maximum precipitation (PMP) estimates. The PMP is transformed into a probable maximum flood hydrograph that is subsequently used to design spillways etc. In this study the PMP values have been estimated using a hydrometeorological method involving depth-area-duration analysis, moisture maximization, and altitude adjustment for typhoon Nina, for 1, 2, and 3 days duration. Areal PMP values were obtained for the entire Hongru river catchment, as well as for the subcatchments upstream the dams at Banqiao (762 km2), Shimantan (230 km2), Boshan (580 km2), and Suyahu (4 498 km2). For point values, the PMP was estimated to 1 200 mm/day, 1 460 mm/2 days, and 1 910 mm/3 days at altitudes about 100 m, which agrees well with previous studies.
    Type of Medium: Electronic Resource
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