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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK; Malden, USA : Munksgaard International Publishers
    Physiologia plantarum 121 (2004), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A versatile two-step cultivation procedure for Arabidopsis thaliana is described for the production of large quantities of leaf material suitable for biochemical and biophysical analysis. The first step comprises a miniature greenhouse made out of a plastic pipette box to grow the seedlings to the six-leaf stage. For continued growth, the seedlings are transferred to hydroponic cultivation using an opaque container covered by a styrofoam lid. Transfer of the small seedlings to hydroponic culture is facilitated by growth in separate pipette tips, which protects vulnerable roots from damage. The hydroponic cultivation system is easy to scale-up and produces large amounts of relatively large leaves and roots. This hydroponic system produces enough plant material to make Arabidopsis a feasible model for biochemical and biophysical experiments, which can be combined with the available genetic information to address various aspects of plant functional genomics.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of advanced nursing 25 (1997), S. 0 
    ISSN: 1365-2648
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The aim of this study was to investigate the relationship between the performance of breast self-examiniation (BSE) and age, place of residence, ethnic background and religion, as well as whether a group willing to take part in an interview regarding BSE mirrored the whole population. Six hundred and ninety-four (69.4%) women, aged 25–80, responded to the questionnaire. Of gthese, 69.6% examined their breasts. Whether the subjects lived in urban or rural areas was of no importance for practising BSE, nor was country of birth or number of years in Sweden. A larger proportion of women aged 45–80 practised BSE compared with women aged 25–44 (P 〈 0.001), the former practising BSE once a month or more. Step-wise logistic regression analysis showed that, for the whole sample as well as for the interview group, age was the only significant predictor of breast self-examination (P 〈 0.001). There were no significant differences between the whole sample and the interview group. The importance of younger women performing the examiniation is stressed. Dur to nurses being strategically located in a wide range of geographical locations, allowing them to meet women in different settings, they are a good choice for motivating women to prctise BSE.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé L'incidence des lésions du nerf laryngé supérieur (NLS) et leur conséquences sur la voix ont été étudiées chez 20 patients ayant eu une chirurgie thyroïdienne par des chirurgiens non spécialistes en chirurgie endocrine. La fonction NLS a été étudiée par électromyographie (EMG) des muscles cricothyroïdiens avant et après l'acte chirurgical. Ont été analysés: (a) la fréquence moyenne, (b) les fréquences extrêmes de la voix parlée normale, et (c) la fréquence limite supérieure. La qualité de la voix a été appréciée par des auditeurs entraînés. Un questionnaire concernant les problèmes de la voix a été rempli par tous les patients. Tous les patients ont eu un examen laryngoscopique indirect avant et après l'acte chirurgical. Trois patients, n'ayant aucun antécédent de chirurgie cervicale, présentaient déjà des lésions partielles du NLS avant la chirurgie thyroïdienne. Selon les données EMG, aucun patient à risque n'a complété de paralysie du NLS après la chirurgie. Cependant, 58% des NLS exposés au risque ont présenté des lésions partielles du NLS en postopératoire, persistant chez tous les patients sauf un, à un an. Sept des 10 patients présentant des lésions isolées du NLS ont présenté des problèmes vocaux postopératoires, qui ont disparu chez 4 à un an. L'examen laryngoscopique indirect classique n'a pas permis de diagnostiquer de lésion isolée du NLS. La présence de lésion du NLS était corrélée significativement (p 〈 0.05) avec une baisse de la fréquence moyenne de la voix parlée. Les lésions partielles du NLS ne peuvent être détectées uniquement par la Symptomatologie vocale ou l'examen du larynx de routine. Seul l'électromyogramme permet un diagnostic précis. La mesure de la fréquence moyenne de la voix peut cependant servir comme examen non invasif de dépistage.
    Abstract: Resumen La incidencia de lesiones del nervio laríngeo superior (NLS) y sus consecuencias en cuanto a calidad de la voz fue estudiada en 20 pacientes sometidos a cirugía tiroidea por cirujanos no especialistas en cirurgía endocrina. La función del NLS fue valorada mediante electromiografía (EMG) de los músculos cricotiroideos antes y después de la cirurgía. El análisis de voz fue realizado mediante el registro del tono promedio y el rango de la voz hablada y el límite máximo del tono. La calidad percibida de la voz fue juzgada por un panel de oyentes. Los problemas de voz fueron registrados a través de un cuestionario. El estado de la laringe fue examinado por laringoscopia indirecta antes y después de la cirurgía. Très pacientes que no habían tenido operaciones sobre el cuello previamente presentaban lesiones parciales del NLS antes de la cirugía. Según la EMG, ningún NLS en riesgo desarrolló lesión completa, pero 58% de los NLS's desarrollaron lesiones parciales que persistieron por un ano después de la cirugía en todos menos uno de los pacientes examinados. Siete de 10 pacientes con lesiones aisladas del NLS presentaron problemas postoperatorios de voz, los cuales habían desaparecido después de un ano en 4 de ellos. La laringoscopia indirecta estandar falló en establecer el diagnóstico de una paresia parcial aislada del NLS. Se hallo una correlación significativa entre la lesión del NLS y una reductión en el tono promedio de la voz hablada (p 〈 0.05). Las lesiones parciales del NLS no pueden ser diagnosticadas exclusivamente con base en síntomas de voz y la laringoscopia indirecta estandar. Los registros de EMG hacen posible el diagnóstico definitivo. Las mediciones del tono promedio de la voz pueden ser utilizadas como procedimientos de tamizaje no invasivos.
    Notes: Abstract The incidence of superior laryngeal nerve (SLN) lesions and their consequences in terms of voice quality was studied in 20 patients undergoing thyroid surgery, performed by surgeons who were not specialists in endocrine surgery. The SLN function was examined by electromyography (EMG) of the cricothyroid muscles before and after surgery. Voice analysis was performed through the registration of the average pitch and range of the speaking voice and the upper pitch limit. The perceptual voice quality was judged by a panel of listeners. Voice problems were registered through a questionnaire. The larynx status was examined by indirect laryngoscopy before and after surgery. Three patients who had not previously been operated on in the neck had partial SLN lesions before surgery. According to the EMG, no SLN at risk developed a complete lesion, but 58% of the SLN's developed partial lesions which persisted 1 year after surgery in all but 1 of the examined patients. Seven of 10 patients with isolated SLN lesions had postoperative voice problems, which had disappeared after 1 year in 4 of them. Standard indirect laryngoscopy failed to diagnose an isolated partial SLN paresis. A significant correlation was found between SLN lesion and a reduction in the average pitch of the speaking voice (p 〈 0.05). Partial SLN lesions cannot be diagnosed exclusively on voice symptoms and standard indirect laryngoscopy. EMG registrations make a definite diagnosis possible. Measurements of the average pitch of the voice could be used as a noninvasive screening procedure.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Bioscience reports 19 (1999), S. 499-509 
    ISSN: 1573-4935
    Keywords: Arabidopsis ; auxiliary enzymes ; light stress ; photosynthesis ; protein phosphorylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An improved cultivation system for Arabidopsis thaliana was developed, allowing advanced biochemical studies in vitro and in vivo of this important model plant. Highly functional Arabidopsis thylakoids were isolated and used to study both basic and regulatory photosynthetic functions with the aim to create a platform for the characterization of mutants deficient in auxiliary proteins. Light-induced proteolytic degradation of the D1 protein could be followed and shown to be a subsequent event to photoinactivation of electron transport. The phosphorylation and dephosphorylation of thylakoid proteins resembled that seen in spinach leaves although phospho-CP43 revealed an unusual regulatory behavior.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 81 (1988), S. 155-163 
    ISSN: 1573-4919
    Keywords: chloroplast ; thylakoid ; membrane ; inside-out vesicles ; photosystem II ; photosystem I ; counter current distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Inside-out thylakoid vesicles were isolated from spinach chloroplasts, and fragmented by sonication. Different fragments were separated by counter-current distribution and analyzed for chlorophyll and P700. The inside-out vesicles had a chlorophyll a/b ratio of 2.2–2.4 (original chloroplasts 2.8–3.0). After further fragmentation of the inside-out vesicles by sonication and separation by countercurrent distribution three populations of vesicles were obtained having chlorophyll a/b ratios of 1.7, 1.9 and 2.5 respectively. The P-700 was depleted in fractions with lower chlorophyll a/b ratio and was nearly absent in the fraction having a chlorophyll a/b ratio of 1.7 (chlorophyll/P700 〉 4500 mol/mol). That PSII membrane vesicles, with such a low chlorophyll a/b ratio and lacking PSI, can be prepared by a non-detergent method provides strong support for the notion that PSI and PSII are segregated along the thylakoid membrane. A plot of P700 per chlorophyll against chlorophyll b/(a+b) fits a straight line connecting the pure PSI membrane (chlorophyll a/b = 6; P700/chlorophyll = 5.6 mmol/mol) with the pure PSII membrane (chlorophyll a/b = 1.7; P700 = 0). These two membranes can be considered as separate phases of a two-dimensional phase system. Models for the thylakoid membrane are discussed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 20 (1989), S. 249-259 
    ISSN: 1573-5079
    Keywords: oxygen evolution ; phase partitioning ; photosystem II ; P700 ; sonication ; spinach chloroplast ; thylakoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An improved, non-detergent, method for preparative isolation of PS II membrane vesicles from spinach chloroplasts is presented. Thylakoids (chlorophyll (Chl) a/b ratio 2.8, Chl/P700 435) were fractionated by Yeda press treatment and aqueous two-phase partition to yield inside-out vesicles (1) (chl a/b 2.2, chl/P700 700). These vesicles were subjected a sonication — phase partitioning procedure; steps of sonication of inside-out vesicles, while still present in a dextran-polyethylene glycol two-phase system were alternated by phase partition. These steps selectively removed P700-containing membrane fragments from the inside-out vesicles and yielded a membrane fraction with improved PS II purity (Chl a/b ratio 1.9, Chl/P700 1500) and retained oxygen evolving capacity (295 μmol O2 mg Chl-1 h-1).
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    International journal of stress management 3 (1996), S. 239-253 
    ISSN: 1573-3424
    Keywords: unemployment ; fired manager ; stress-coping-reaction ; Sweden
    Source: Springer Online Journal Archives 1860-2000
    Topics: Psychology
    Notes: Abstract This paper purports to enhance the understanding of stress and its effects on unemployed executives. The idea that unemployed managers perceive and confront stress differently from their employed colleagues is addressed. A sampling of 32 unemployed executives and managers was taken using The Stress Profile, to survey reasons for stress, coping ability, and response to stress. The profiled samples were then compared to a comparison group of 367 employed managers. The results showed that unemployed managers, when compared to their employed associates, tended to have less desireable family situations, more worries about finances, decreased perception of self and self-coherence, decreased coping ability, and heightened emotional and behavioral reaction to stress. The conclusion was that when dealing with stressors, unemployed managers experienced a decreased sense of psychological well-being and coping abilities as compared to employed managers.
    Type of Medium: Electronic Resource
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