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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 10 (1975), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In the present study the hydroxyproline content in gingival exudate and serum obtained from individuals with periodontal disease was determined at three times; before, one month and six months after periodontal surgery. The gingival exudate was collected with filter paper strips according to the intracrevicular method. The hydrolysates of gingival exudate and serum were assayed colorimetrically for hydroxyproline.Although the hydroxyproline content of the exudate and of serum one month after surgery was higher than the values before surgery, the differences were not statistically significant. The values for exudate and serum at six months, however, were significantly lower than at one month; exudate 23.45 ± 13.13 μg/g vs. 39.85 ± 18.63, P 〈 0.05; serum 17.05 ± 2.22 μg/g ml vs. 19.34 ± 2.19, P 〈 0.05. These decreased values at six months postoperatively may reflect a decrease of collagen degradation in the course of gingival tissue repair.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 28 (1993), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Collagenolysis in periodontitis is thought to be modulated by the expression of three genes, collagenase, tissue inhibitors of metalloproteinases-1 and -2 (TIMP-1 and -2). We assessed the possible difference in TIMP-1, TIMP-2 and collagenase mRNA levels between gingival samples from patients with periodontitis and those from healthy subjects by reverse transcription-polymerase chain reaction (RT-PCR). This technique allows detection of transcripts from a very small sample quantity. The experiments showed that levels of TIMP-1 and collagenase transcripts relative to β-action are significantly higher in the diseased group than in healthy controls (8.11±0.83 versus 1.38±0.28% for TIMP-1 and 0.50±0.10 versus 0.0075±0.0024% for collagenase, respectively). The difference in TIMP-2 between the two groups (2.91±0.46 versus 1.84±0.87%) did not differ. Therefore, the host would have responded to the increase in collagenase level by preferentially producing TIMP-1 against tissue destruction. The differential gene expression of TIMP-1 and TIMP-2 in our study may account for a distinct genetic regulation of TIMP-1 and -2 in vivo.
    Type of Medium: Electronic Resource
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