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  • 1
    Electronic Resource
    Electronic Resource
    Heterotypic induction of stomach-like glands in the urogenital sinus endoderm under the influence of stomach mesenchyme of foetal rats (1990)
    Springer
    Development genes and evolution 198 (1990), S. 483-487 
    Details
    ISSN: 1432-041X
    Keywords: Mesenchymal-epithelial interaction ; Stomach ; Heterotypic induction ; Pepsinogen ; Morphogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Urogenital sinus endoderm of 16.5-day rat foetuses was combined with stomach mesenchyme and the recombinants were either treated with testosterone and grown in vitro or cultured beneath the kidney capsule of adult male rats of the same strain. It was found that testosterone stimulated mitosis in the urogenital endoderm. In recombinants grown under the kidney capsule a stratified squamous epithelium and stomach-like glands were induced under the influence of the forestomach and glandular stomach mesenchymes. However, the induced glands expressed neither rat pepsinogen nor rat ventral prostatic antigen. They did not produce mRNA for the prostatic steroid-binding protein C1. Thus, stomach mesenchyme of rat foetuses induces organ-specific morphogenesis but not functional differentiation in the heterologous endoderm, indicating that cytodifferentiation does not always accompany morphogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00399060
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  • 2
    Electronic Resource
    Electronic Resource
    Cell-specific and spatio-temporal expression of intestinal fatty acid-binding protein gene during amphibian metamorphosis (1994)
    Springer
    Development genes and evolution 204 (1994), S. 150-155 
    Details
    ISSN: 1432-041X
    Keywords: Fatty-acid-binding protein ; Intestinal epithelium ; In situ hybridization ; Anuran metamorphosis ; Regional difference
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Intestinal fatty acid-binding protein (IFABP) gene is known to be regulated during Xenopus metamorphosis. To determine the relationship between its regulation and cellular differentiation during metamorphosis, we have examined the distribution of IFABP mRNA in the Xenopus digestive tract by in situ hybridization techniques. Throughout all stages examined, transcripts of IFABP gene were observed exclusively in absorptive epithelial cells of the small intestine, and they decreased in amount towards the posterior intestine. Around stage 58, just before metamorphic climax, IFABP mRNA level began to decrease in larval absorptive cells that still remained intact morphologically. Thereafter, IFABP mRNA was no longer detected among larval cells. In turn, at stage 62, IFABP mRNA became detectable in some of the newly formed adult epithelium that had not yet developed a brush border, but not in the remaining larval cells. By the end of metamorphosis, IFABP mRNA became more abundant towards the crest of intestinal folds. These results suggest that IFABP gene expression is specific for absorptive epithelial cells of the small intestine and is regionally regulated along the intestinal anterior-posterior axis in both tadpoles and frogs and also along the trough-crest axis of frog intestinal folds. In addition, our present study directly shows that IFABP mRNA level decreases in larval absorptive cells but increases in adult ones during metamorphosis, preceding morphological changes of both types of cells. Therefore, the regulation of IFABP gene is an early event during both larval epithelial cell death and adult epithelial cell differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00361110
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  • 3
    Electronic Resource
    Electronic Resource
    Noise-resistant and synchronized oscillation of the segmentation clock (2006)
    [s.l.] : Nature Publishing Group
    Nature 441 (2006), S. 719-723 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Periodic somite segmentation in vertebrate embryos is controlled by the ‘segmentation clock’, which consists of numerous cellular oscillators. Although the properties of a single oscillator, driven by a hairy negative-feedback loop, have been investigated, the system-level properties of the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature04861
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  • 4
    Electronic Resource
    Electronic Resource
    Mesoderm induction in zebrafish (1996)
    [s.l.] : Nature Publishing Group
    Nature 383 (1996), S. 131-132 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR á€" Mesoderm formation and its dorso-ventral specification are essential processes in vertebrate early development. The mesoderm produces many types of tissues such as notochord, muscle, bone and blood cells, arranged in the correct positions along the dorsoventral axis. The ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/383131a0
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  • 5
    Electronic Resource
    Electronic Resource
    Osteocalcin, deoxypyridinoline and interleukin-1β in peri-implant crevicular fluid of patients with peri-implantitis (2002)
    Oxford, UK : Munksgaard International Publishers
    Clinical oral implants research 13 (2002), S. 0 
    Details
    ISSN: 1600-0501
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The aim of the present study was to analyze the levels of osteocalcin, deoxypyridinoline (Dpd) and interleukin-1β as markers of bone metabolism in peri-implant crevicular fluid (PICF) from peri-implantitis patients. PICF was sampled from a total of 34 endosseous titanium implants from 16 patients; nine females (mean age 52.8, range 40–62 years) and seven males (mean age 56.0, range 36–66 years). The implants had been in place for a period of 9–112 months (mean; 35.8 months) since the loading. These sites were categorized as six peri-implantitis, eight peri-implant mucositis and 20 healthy implant. PICF volume from peri-implantitis sites was significantly higher than mucositis and healthy implant sites (P 〈 0.01). Osteocalcin levels in PICF from mucositis sites were significantly higher than healthy implants (P 〈 0.05), whereas peri-implantitis sites were not significantly different from either mucositis or healthy implant sites. Dpd could not be detected in any of the samples examined. IL-1β levels in PICF from peri-implantitis sites were significantly higher than levels from peri-implant mucositis (P 〈 0.05) and healthy implant sites (P 〈 0.01). In conclusion, osteocalcin in PICF may reflect increased local bone turnover around implants. Further, IL-1β should be a useful marker for peri-implant inflammation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0501.2002.130610.x
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  • 6
    Electronic Resource
    Electronic Resource
    Tbx24 , encoding a T-box protein, is mutated in the zebrafish somite-segmentation mutant fused somites (2002)
    [s.l.] : Nature Publishing Group
    Nature genetics 31 (2002), S. 195-199 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Somites are fundamental structures within the paraxial mesoderm of the vertebrate embryo that give rise to the vertebrae and muscle of the trunk and tail. Studies of knockout mice and gene expression analyses have shown that the Notch pathway is crucial in establishing the reiterative pattern of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng899
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  • 7
    Electronic Resource
    Electronic Resource
    Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments (2000)
    [s.l.] : Nature America Inc.
    Nature biotechnology 18 (2000), S. 62-65 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have established a transgenic zebrafish line carrying a shuttle vector plasmid (pML4) for detecting mutagens in aquatic environments. The plasmid contains the rpsL gene of Escherichia coli as a mutational target gene, and the kanamycin-resistance gene for recovering the plasmid from the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/71938
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  • 8
    Electronic Resource
    Electronic Resource
    Selective production of glutamate by an immobilized marine blue-green alga, Synechococcus sp. (1988)
    Springer
    Applied microbiology and biotechnology 28 (1988), S. 373-376 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Among 200 strains of marine bluegreen algae isolated from the coastal areas of Japan, the marine blue-green alga Synechococcus sp. NKBG 040607 excreted glutamate at the highest rate, 82.6% of total amino acids production being glutamate. Synechococcus sp. NKBG 40607 was immobilized in calcium alginate gel. Glutamate production by immobilized cells was double that of native cells. Maximal glutamate production (25 μg/cm3 gel per day) of the immobilized cells was observed under a light intensity of 144 μEinstein/m2 per second at a cell concentration of 7.5 mg dry cells/cm3 gel. Immobilized cells of Synechococcus sp. can use nitrate as a nitrogen source. Immobilized marine Synechococcus sp. produced 0265 mg/cm3 gel of glutamate for 7 days in the presence of chloramphenicol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268198
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  • 9
    Electronic Resource
    Electronic Resource
    Heat tolerance in food legumes as evaluated by cell membrane thermostability and chlorophyll fluorescence techniques (1996)
    Springer
    Euphytica 88 (1996), S. 35-45 
    Details
    ISSN: 1573-5060
    Keywords: Chickpea ; chlorophyll fluorescence ; groundnut ; heat tolerance ; membrane stability ; photosystem ; pigeonpea ; screening methods ; soya bean ; thermostability ; Cicer arietinum ; Glycine max ; Arachis hypogaea ; Cajanus cajan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genotypic variation for heat tolerance in chickpea, groundnut, pigeonpea, and soya bean was evaluated by testing membrane stability and photosystem (PSII) function in leaves at high temperatures. The legumes could be ranked from heat-tolerant to sensitive in the order: groundnut, soya bean, pigeonpea and chickpea. The damage to cell membranes (as reflected by an increased leakage of electrolytes) and PS II (as reflected by a decrease in the ratio of variable to maximum fluorescence) was less, and recovery from heat stress was faster in groundnut than in other crops. Prior exposure of plants to 35°C for 24h led to a reduced leakage of electrolytes at high temperatures in all crops but the differences among legumes were consistent. Substantial genotypic variation for heat tolerance was found in all legumes. Membrane injury was negatively associated with specific leaf weight in groundnut (r=−0.69**) and soya bean (r=−0.56**) but not in the pulses. Electrolyte leakage and fluorescence ratio were negatively correlated in all legumes. The potential use of electrolyte leakage and fluorescence tests as screening procedures for breeding heat-tolerant legumes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029263
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  • 10
    Electronic Resource
    Electronic Resource
    Renaturation and activity staining of glycosidases and glycosyltransferases in gels after sodium dodecyl sulfate-electrophoresis (1994)
    Weinheim : Wiley-Blackwell
    Electrophoresis 15 (1994), S. 911-915 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Glycosidases and glycosyltransferases were electrophoresed in the presence of sodium dodecyl sulfate (SDS) in a thin-layer gel supported by a glass plate, treated with the nonionic detergent Triton X-100, and specifically stained for the sugar-releasing activity of these enzymes. Staining is based on conversion of monosugars or a sugar phosphate to glucose-6-phosphate by the appropriate intermediary enzymes, reduction of NADP+ to NADPH, and accumulation of reduced Nitroblue Tetrazolium in the gel. Among the enzymes tested, α-glucosidase, β-glucosidase and β-mannosidase could not be renatured, whereas β-fructofuranosidase and α-mannosidase could be renatured unless heated before electrophoresis. Sucrose phosphorylase, glucosyltransferase and fructosyltransferase, which are single-peptide proteins with no cystine bond, could be renatured even after pretreatment with SDS and/or mercaptoethanol at 100°C for 10 min. However, exclusive heating remarkably decreased the activities of these enzymes. Two-dimensional separation of the five renaturable enzymes was done in a single thin-layer gel, using SDS-electrophoresis in the first dimension and isoelectric focusing in the second dimension.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501501131
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    Paper (German National Licenses)
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