ISSN:
1420-908X
Keywords:
Key words: Antioxidants — Ca2+-ATPase inhibitors — TNF-α— RBL-2H3 cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract. Objective and Design: To investigate the effect of Ca2+-ATPase inhibitors on the production of TNF-α in rat basophilic leukemia (RBL-2H3) cells.¶Material: Two Ca2+-ATPase inhibitors, thapsigargin (TG) and cyclopiazonic acid (CPA), and three hydroquinone-antioxidants, 2,5-di-(tert-butyl)-1,4-hydroquinone (DTBHQ), 2,5-di-(tert/amyl)-1,4-hydroquinone (DTAHQ), 2-(tert-butyl)-1,4-hydroquinone (MTBHQ) were used.¶Treatment: Cells were treated with TG, CPA, DTBHQ, DTAHQ and MTBHQ for 3 h in the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) and released TNF-α from the cells was measured (n 〉 or = 4).¶Results: All Ca2+-ATPase inhibitors (TG, CPA, DTBHQ and DTAHQ) induced TNF-α release in a dose-dependent manner. TNF-α release was inhibited by treatment with protein kinase C inhibitors (staurosporine, Ro31-8220, calophostin C) (p 〈 or = 0.05). In contrast, MTBHQ, which does not induce increases in [Ca2+]i, did not induce the release of TNF-α. TNF-α release induced by DTBHQ and CPA was inhibited by treatment with actinomycin-D, the immunosuppressant FK506 and the glucocorticoid dexamethasone (p 〈 or = 0.01).¶Conclusions: These results suggest 1) that [Ca2+]i increase and subsequent activation of protein kinase C is necessary for the release of TNF-α, and they work synergistically, 2) that the TNF-α release induced by Ca2+-ATPase inhibitors can be regulated at the transcriptional level.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s000110050337
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