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  • 1
    Electronic Resource
    Electronic Resource
    Technique of preparing powder samples for AES [Auger electron spectroscopy] and/or ESCA analysis (1975)
    s.l. : American Chemical Society
    Analytical chemistry 47 (1975), S. 1492-1493 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac60358a042
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Regional variation in adipose tissue insulin action and GLUT4 glucose transporter expression in severely obese premenopausal women (1997)
    Springer
    Diabetologia 40 (1997), S. 590-598 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Adipocyte ; fat ; glucose transport ; lipolysis ; adenosine.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Insulin action and GLUT4 expression were examined in adipose tissue of severely obese premenopausal women undergoing gastrointestinal surgery. Fat samples were taken from three different anatomical regions: the subcutaneous abdominal site, the round ligament (deep abdominal properitoneal fat), and the greater omentum (deep abdominal intraperitoneal fat). The stimulatory effect of insulin on glucose transport and the ability of the hormone to inhibit lipolysis were determined in adipocytes isolated from these three adipose depots. Insulin stimulated glucose transport 2–3 times over basal rates in all adipocytes. However, round ligament adipose cells showed a significantly greater responsiveness to insulin when compared to subcutaneous and omental adipocytes. Round ligament fat cells also displayed the greatest sensitivity and maximal antilipolytic response to insulin. We also investigated whether regional differences in fat cell insulin-stimulated glucose transport were linked to a differential expression of the GLUT4 glucose transporter. GLUT4 protein content in total membranes was 5 and 2.2 times greater in round ligament adipose tissue than in subcutaneous and omental fat depots, respectively. Moreover, GLUT4 mRNA levels were 2.1 and 3 times higher in round ligament than in subcutaneous or omental adipose tissues, respectively. Adipose tissue GLUT4 protein content was strongly and negatively associated (r = –0.79 to –0.89, p 〈 0.01) with the waist-to-hip ratio but not with total adiposity. In conclusion, these results demonstrate the existence of site differences in adipose tissue insulin action in morbidly obese women. The greater insulin effect on glucose transport in round ligament adipocytes was associated with a higher expression of GLUT4 when compared to subcutaneous abdominal and omental fat cells. Moreover, despite the regional variation in GLUT4 expression, an increased proportion of abdominal fat was found to be associated with lower levels of GLUT4 in all adipose regions investigated. [Diabetologia (1997) 40: 590–598]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050720
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  • 3
    Electronic Resource
    Electronic Resource
    Are gender differences in cardiovascular disease risk factors explained by the level of visceral adipose tissue? (1994)
    Springer
    Diabetologia 37 (1994), S. 757-764 
    Details
    ISSN: 1432-0428
    Keywords: Visceral fat ; sex dimorphism ; lipoprotein glucose metabolism ; computed tomography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It has been suggested that the lower prevalence of cardiovascular disease in women before menopause in comparison with men may be explained by differences in body fat distribution, plasma lipoprotein levels and indices of plasma glucose-insulin homeostasis. Thus, gender differences in visceral adipose tissue accumulation measured by computed tomography and metabolic variables were studied in 80 men and 69 pre-menopausal women, aged 23–50 years. Despite the fact that women had higher levels of total body fat (p〈0.0001), they displayed lower areas of abdominal visceral adipose tissue (p〈0.06) and a lower ratio of abdominal visceral to mid-thigh adipose tissue areas than men (p〈0.0001). After adjustment for body fat mass, women generally displayed a more favourable risk profile than men which included higher plasma HDL2-cholesterol and lower plasma insulin, apolipoprotein B and triglyceride levels (p〈0.01). Metabolic variables adjusted for body fat mass were then compared between genders after control for differences in abdominal visceral adipose tissue area. After such controls, variables related to plasma glucose-insulin homeostasis were no longer significantly different between men and women. Gender differences for plasma concentrations of triglyceride, apolipoprotein B and the ratio of HDL2-cholesterol/HDL3-cholesterol also disappeared, whereas plasma concentrations of HDL-cholesterol, HDL2-cholesterol as well as the ratio of HDL-cholesterol/total cholesterol remained significantly higher in women than in men (p〈0.01). These results suggest that abdominal visceral adipose tissue is an important correlate of gender differences in cardiovascular disease risk. However, additional factors are likely to be involved in gender differences in plasma HDL-cholesterol levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404332
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  • 4
    Electronic Resource
    Electronic Resource
    Are gender differences in cardiovascular disease risk factors explained by the level of visceral adipose tissue? (1994)
    Springer
    Diabetologia 37 (1994), S. 757-764 
    Details
    ISSN: 1432-0428
    Keywords: Key words Visceral fat, sex dimorphism, lipoprotein, glucose metabolism, computed tomography.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It has been suggested that the lower prevalence of cardiovascular disease in women before menopause in comparison with men may be explained by differences in body fat distribution, plasma lipoprotein levels and indices of plasma glucose-insulin homeostasis. Thus, gender differences in visceral adipose tissue accumulation measured by computed tomography and metabolic variables were studied in 80 men and 69 pre-menopausal women, aged 23–50 years. Despite the fact that women had higher levels of total body fat (p〈0.0001), they displayed lower areas of abdominal visceral adipose tissue (p〈0.06) and a lower ratio of abdominal visceral to mid-thigh adipose tissue areas than men (p〈0.0001). After adjustment for body fat mass, women generally displayed a more favourable risk profile than men which included higher plasma HDL2-cholesterol and lower plasma insulin, apolipoprotein B and triglyceride levels (p〈0.01). Metabolic variables adjusted for body fat mass were then compared between genders after control for differences in abdominal visceral adipose tissue area. After such controls, variables related to plasma glucose-insulin homeostasis were no longer significantly different between men and women. Gender differences for plasma concentrations of triglyceride, apolipoprotein B and the ratio of HDL2-cholesterol/HDL3-cholesterol also disappeared, whereas plasma concentrations of HDL-cholesterol, HDL2-cholesterol as well as the ratio of HDL-cholesterol/total cholesterol remained significantly higher in women than in men (p〈0.01). These results suggest that abdominal visceral adipose tissue is an important correlate of gender differences in cardiovascular disease risk. However, additional factors are likely to be involved in gender differences in plasma HDL-cholesterol levels. [Diabetologia (1994) 37: 757–764]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050176
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Cloning of Pseudomonas plasmid pMG7 and its restriction-modification system in Escherichia coli
    Amsterdam : Elsevier
    Gene 19 (1982), S. 355-359 
    Details
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; cosmid ; in vitro packaging ; pHC79 ; transduction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(82)90026-9
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  • 6
    Electronic Resource
    Electronic Resource
    Treatment with chitosan enhances resistance of tomato plants to the crown and root rot pathogen Fusarium oxysporum f. sp. radicis-lycopersici
    Amsterdam : Elsevier
    Physiological and Molecular Plant Pathology 41 (1992), S. 33-52 
    Details
    ISSN: 0885-5765
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0885-5765(92)90047-Y
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of exercise-training and detraining on fat cell lipolysis in men and women (1984)
    Springer
    European journal of applied physiology 53 (1984), S. 25-30 
    Details
    ISSN: 1439-6327
    Keywords: adipocytes ; Lipolysis ; Body fatness ; Training ; Detraining ; Sex difference
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of training and detraining on adipose tissue lipolysis were studied in 19 healthy subjects (7 women and 12 men) who were submitted to a 20-week aerobic training program. Thereafter, subjects refrained from exercise for a period of 50 days. Suprailiac fat biopsies were performed before training, after training, and at the end of the detraining period. Mean fat cell diameter and epinephrine stimulated lipolysis (ESL) were assessed on collagenase isolated fat cells. Body density through underwater weighing and skinfolds at seven different sites were also obtained. Training significantly increased ESL (P〈0.05) in men but not in women. However, ESL values in men returned to pretraining values after the exercise restriction period. No significant changes in women lipolysis were observed under any conditions. Changes in lipolysis were not correlated with changes in body fatness. However, a significant correlation was observed between the increase in ESL produced by training and the subsequent decrease caused by detraining (r=−0.53;P〈0.05). The present results suggest that lipolysis in fat cells from the female subjects seems to be insensitive to changes in energy expenditure. Moreover, the present study demonstrates that there are high and low responders in adipocytes ESL to variations in habitual energy expenditure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00964685
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    Paper (German National Licenses)
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