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  • 1
    Electronic Resource
    Electronic Resource
    Silver enhancement of protein A-gold probes on resin-embedded ultrathin sections (1987)
    Springer
    Histochemistry and cell biology 86 (1987), S. 371-373 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A simple method is described allowing the enhancement of the visibility of small gold probes for the electron microscopy. This method, which allows the silver intensification of gold directly on epon-embedded ultrathin sections, was used for the electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens in cultured cells derived from GR and BALB/cfRIII mouse mammary tumors. After the immunostaining with the preembedding protein A-gold technique, the ultrathin sections, placed on 200 mesh copper grids, were rehydrated and exposed to a photographic developer containing silver nitrate. During this physical development gold particles are incapsulated in growing shells of metallic silver, which gradually become more and more visible. We were able to obtain a heavy labelling of the viral particles, well visible even at low magmfication, with a negligeable background staining. The present technique can be useful whenever it is necessary to use the smallest gold probes today available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494995
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of metergoline, a powerful and long-acting antiserotoninergic agent, on insulin secretion in normal subjects and in patients with chemical diabetes (1975)
    Springer
    Diabetologia 11 (1975), S. 165-167 
    Details
    ISSN: 1432-0428
    Keywords: Serotonin ; 5-hydroxytryptamine ; metergoline ; arginine ; antiserotoninergic drugs ; insulin secretion ; glucose tolerance test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of metergoline on insulin secretion has been evaluated in normal subjects and in patients with chemical diabetes. The repeated administration of metergoline, 2 mg at four-hour intervals to give a total of 24 mg, has enhanced insulin secretion in response to i. v. glucose in normal subjects but not in chemical diabetics. No changes in blood glucose pattern were observed. Under similar conditions, metergoline administration caused a slight but significant decrease in arginine-induced insulin release, both in normal subjects and in chemical diabetics. These results support the concept of a serotoninergic control of insulin secretion and suggest that serotonin exerts different effects on insulin release according to the different stimuli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429842
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Behçet's disease with gastrointestinal involvement associated with myelodysplasia in a patient with congenital panhypopituitarism (1998)
    Springer
    Clinical rheumatology 17 (1998), S. 515-517 
    Details
    ISSN: 1434-9949
    Keywords: Behçet's disease ; Congenital panhypopituitarism ; Myelodysplastic syndromes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Myelodysplastic syndromes (MDS) are hematologic disorders characterised by peripheral cytopenias and the hystologic features of hematologic dysplasia. Their association with autoimmune manifestations have been suggested by several authors: recently, the appearance of Behçet's disease during MDS has been reported. We describe the occurrence of MDS in a patient affected by Behçet's syndrome with gastrointestinal involvement and congenital panhypopituitarism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01451290
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    Paper (German National Licenses)
    Fulltext
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