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  • 1
    Electronic Resource
    Electronic Resource
    Water relations of higher plants (1980)
    Springer
    European biophysics journal 6 (1980), S. 67-67 
    Details
    ISSN: 1432-1017
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00647539
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Extraction and analysis of sap from individual wheat leaf cells: the effect of sampling speed on the osmotic pressure of extracted sap (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 12 (1989), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. A modification to the pressure probe is described which allows very rapid extraction of sap samples from single higher plant cells. The performance of this rapid-sampling probe was assessed and compared with the unmodified probe for cells of both wheat and Tradescantia. Under some conditions, the unmodified probe operated too slowly to avoid dilution of cell sap during the extraction process. This led to values for apparent sample osmotic pressures that were below the turgor pressures for the same cells. The problem was particularly acute in young wheatleaf epidermal cells which are small, elongate and have high turgor pressure. These exhibited rapid water influx when their turgor was depressed during the sampling of their contents (half-time for pressure recovery in wheat cells was less than 1 s while in Tradescantia cells it was 3–5 s). Dilution during sampling was apparently negligible when the rapid sampling probe was used. The study was complemented by a simple model of the way cells dilute during sampling. Quantitative predictions of the model were consistent with our observed findings. The model is used to assess the major factors which determine a cell's susceptibility to dilution during sampling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1989.tb01971.x
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  • 3
    Electronic Resource
    Electronic Resource
    Control of wheat root growth. The effects of excision on growth, wall rheology and root anatomy (1988)
    Springer
    Planta 176 (1988), S. 399-405 
    Details
    ISSN: 1432-2048
    Keywords: Growth (root, excision) ; Pressure probe ; Root growth (excision effects) ; Triticum (root growth) ; Turgor pressure ; Wall extensibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Excision and subsequent incubation of the apices (1 cm) of wheat (Triticum aestivum L.) seedling roots in simple media severely reduced elongation from 28 mm·(24 h)-1 in intact roots to a maximum of 2 mm·(24 h)-1 in excised roots. The reduction in growth was accompanied by a loss of cell turgor in the growing zone but was correlated with a hardening of the cell walls in this region. Rheological properties were measured as percent extensibility (both plastic and elastic) using a tensiometer, and as instantaneous volumetric elastic modulus (ε i) using the pressure probe. Excision decreased plastic and elastic properties with a half-time of some 60 min. Plastic extension was reduced from 2.5% to 0.9% and elastic from 4.8% to 2.6% for an 8-g load. By contrast, ε i was increased by excision. The observed reduction in root elongation rate was accompained by a reduction in mature cell length from 240 μm to 40 μm and a shortening of the zone of cell expansion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395421
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  • 4
    Electronic Resource
    Electronic Resource
    A simple pressure-probe method for the determination of volume in higher-plant cells (1990)
    Springer
    Planta 182 (1990), S. 199-203 
    Details
    ISSN: 1432-2048
    Keywords: Cell volume ; Pressure probe ; Tradescantia (cell volume) ; Triticum (cell volume)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method is described for estimating volume in higher-plant cells from their behaviour under pressure probe. The method utilises differences between initial and final equilibrium turgor pressures associated with a pressure-relaxation experiment. The validity of the approach is tested using model parameters, and by comparing cell volumes obtained using the method with those estimated from direct visual inspection of the same cells. The range of practical application is limited by certain cell parameters, especially osmotic pressure. Nevertheless the method will be useful for many types of higher-plant cell, particularly those of irregular shape or those that are deeply embedded within plant tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197111
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  • 5
    Electronic Resource
    Electronic Resource
    Salt tolerance in the halophyte Suaeda maritima L. Dum. (1985)
    Springer
    Planta 165 (1985), S. 392-396 
    Details
    ISSN: 1432-2048
    Keywords: Salt tolerance ; Osmotic adjustment ; Turgor ; Suaeda (salt tolerance)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Osmotic potentials and individual epidermal cell turgor pressures were measured in the leaves of seedlings of Suaeda maritima growing over a range of salinities. Leaf osmotic potentials were lower (more negative) the higher the salt concentration of the solution and were lowest in the youngest leaves and stem apices, producing a gradient of osmotic potential towards the apex of the plant. Epidermal cell turgor pressures were of the order of 0.25 to 0.3 MPa in the youngest leaves measured, decreasing to under 0.05 MPa for the oldest leaves. This pattern of turgor pressure was largely unaffected by external salinity. Calculation of leaf water potential indicated that the gradient between young leaves and the external medium was not altered by salinity, but with older leaves, however, this gradient diminished from being the same as that for young leaves in the absence of NaCl, to under 30% of this value at 400 mM NaCl. These results are discussed in relation to the growth response of S. maritima.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392237
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic dissection of root growth in rice (Oryza sativa L.). II: mapping quantitative trait loci using molecular markers (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 143-152 
    Details
    ISSN: 1432-2242
    Keywords: Key words Drought resistance ; QTLs ; RFLPs ; Cell division ; Root growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Drought is a major abiotic stress of upland rice, and good root growth has been associated with drought avoidance. We report on the genetic mapping of root growth traits in an F2 population derived from two drought-resistant rice varieties, ‘Bala’ and ‘Azucena’. Restriction fragment length polymorphism (RFLP) between the parents was 32%, and a molecular map with 71 marker loci and 17 linkage groups covering 1280 cM was produced. Quantitative trait loci (QTLs) for eight root growth characteristics were mapped using phenotype data obtained in a hydroponic screen previously described in a companion paper. Using a significance threshold of LOD 2.4, we observed one QTL for maximum root length after 28 days growth on chromosome 11. It had a LOD score of 6.9, explained nearly 30% of the variation and appeared to be largely additive in effect. QTLs for maximum root length after 3, 7, 14 and 21 days of growth were also revealed. Some root-length QTLs, including that on chromosome 11, varied greatly with developmental stage. One QTL for root volume and two QTLs for adventitious root thickness were detected. No QTLs were detected for the length of cells in the mature (fully expanded) zone of adventitious root tips. The results obtained are discussed in the context of previous reports on mapping root growth parameters in rice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050542
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic dissection of root growth in rice (Oryza sativa L.) I: a hydrophonic screen (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 132-142 
    Details
    ISSN: 1432-2242
    Keywords: Key words Drought resistance ; RFLPs ; Cell division ; Genetic analysis ; Root growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Root growth is an important component of the adaptation of rice to drought-prone environments. A hydroponic screen was used to study root growth of 28 rice varieties. Both maximum root length and adventitious root thickness varied widely between varieties. In general, japonica varieties had larger root systems than indica varieties. Two F2 populations involving the thick- and long-rooted upland japonica variety ‘Azucena’ and two poor-rooting varieties, namely the upland indica‘Bala’ and the Italian japonica‘Maratelli’, were made and screened in hydroponics. Generation means analysis revealed significant additive and dominance main effects for the root length traits with a prevalence of dominance gene effects in both crosses. The dominance×dominance type of non-allelic interactions were important for maximum root length from day 7 to day 28, root volume, root thickness and root cell length in the cross ‘Bala’בAzucena’. The heritability (broad-sense) estimates varied from low to high for the traits and displayed differences between populations. This suggested that recombinant lines with improved root traits can be developed from the two crosses with selection methods that involve some form of progeny evaluation. In a companion paper, we report the mapping of quantitative trait loci (QTLs) for root growth traits in the ‘Bala’בAzucena’ population using restriction fragment length polymorphisms (RFLPs).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050541
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