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  • 1
    Electronic Resource
    Electronic Resource
    Egg White Lysozyme Purification by Ultrafiltration and Affinity Chromatography (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 58 (1993), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Isolation and purification of lysozyme from hen egg white was studied using a two-step procedure. The egg white was diluted 5- to 9-fold with sodium phosphate buffer, and then processed by sequential dilution diafiltration using a UF membrane (molecular weight cut-off 300,000 dalton). The membrane process increased the specific activity of lysozyme 6-fold, and recovered 96% of lysozyme activity. The permeate from diafiltration was further purified by affinity chromatography using chitin as adsorbent. The second step of the process yielded a product of specific activity of 70,400 units/mg protein. The overall lysozyme recovery was 79%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1993.tb04261.x
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  • 2
    Electronic Resource
    Electronic Resource
    Correspondence. Thermal Conductivity of Two-Phase Materials (1962)
    s.l. : American Chemical Society
    Industrial and engineering chemistry 1 (1962), S. 222-223 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/i160003a013
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  • 3
    Electronic Resource
    Electronic Resource
    Monitoring and control of methanol concentration during polysaccharide fermentation using an on-line methanol sensor (1992)
    Springer
    Bioprocess and biosystems engineering 7 (1992), S. 241-247 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Combining principles of membrane separation and semiconductor gas sensor technology, we constructed a methanol sensor to follow methanol concentrations on-line. A length of silicone tubing allowed for mass transfer of methanol from the fermentation medium to a carrier gas which then flowed over a semiconductor gas sensor for detection. The sterilizable sensor demonstrated excellent ability in following methanol concentrations during the batch production of a polysaccharide by the organism Methylomonas mucosa, even as the fermentation broth became increasingly viscous. During fed-batch control by feeding methanol to the fermentation to maintain setpoint methanol levels, a drift in the sensor signal was noted and quantified. A drift factor was determined which, after it was incorporated into the calibration calculations, improved methanol concentration control greatly. Methanol concentration was held constant over a range of set point concentrations during fedbatch fermentations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386233
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  • 4
    Electronic Resource
    Electronic Resource
    Fructose production from sucrose and high fructose syrup: A mycelial fungal system (1982)
    Springer
    Biotechnology letters 4 (1982), S. 353-358 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The use of Mucor sp. M105 and Fusarium sp. F5 in the production of fructose from sugarcane sucrose and high fructose syrup (HFS) was investigated. Although Mucor sp. could not utilize sucrose as the sole carbon and energy source for cell growth, Mucor sp. preferentially utilized glucose in a glucose:fructose (1:1) mixture during fermentation to ethanol. In contrast, Fusarium sp. utilized sucrose as sole carbon source by secretion of extracellular hydrolytic enzymes that degraded the disaccharide. In Fusarium sp., glucose formation in the medium was faster than fructose. Due to the low consumption rate of fructose, this substrate remained in the fermentation broth. The application of these biological systems for the production of fructose from either sucrose or HFS is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00135421
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  • 5
    Electronic Resource
    Electronic Resource
    Ethanol inhibition of D-xylulose fermentation by Schizosaccharomyces pombe (1984)
    Springer
    Biotechnology letters 6 (1984), S. 7-12 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The kinetics of the utilization of D-xylulose by the yeast Schizosaccharomyces pombe has been examined under anaerobic batch conditions. The inhibitory effect of ethanol on xylulose uptake and ethanol production was studied at pH 6.0 and 30°C. Ethanol had little or no effect on the sugar uptake rate, but end product inhibition was observed on ethanol production. This non-competitive inhibition was linear with respect to ethanol concentration between 0 and 60 g/l. A kinetic model for the alcoholic fermentation of xylulose is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00128222
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  • 6
    Electronic Resource
    Electronic Resource
    A model of acetic acid and 2,3-butanediol inhibition of the growth and metabolism ofKlebsiella oxytoca (1985)
    Springer
    Biotechnology letters 7 (1985), S. 727-732 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The main product of fermentation byKlebsiella oxytoca is 2,3-butanediol. This organism also produces acetic acid, ethanol, and acetoin. In this report, product inhibition due to 2,3-butanediol and acetic acid is considered. Although the acetate ion has little effect on growth, acetic acid is a strong inhibitor. Acetic acid inhibits growth more strongly than it inhibits respiration. The neutral product 2,3-butanediol is not a strong inhibitor; its effect on growth is no more than is expected by the decrease in water activity it causes. The effect of 2,3-butanediol on respiration can also be explained by a decreased water activity. It appears that it is possible to accumulate as much as 130 g/L butanediol while as little as 0.45 g/L acetic acid completely inhibits growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01032284
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  • 7
    Electronic Resource
    Electronic Resource
    Use of porous glass fiber as a support for biocatalyst immobilization (1986)
    Springer
    Biotechnology letters 8 (1986), S. 785-790 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Porous glass fiber has a very high surface area and good mechanical properties that make it an excellent support for biocatalyst immobilization. By packing aligned glass fibers in a tubular reactor such that the fibers are all parallel to the axis of the tube, the resulting pressure drop is considerably smaller than for a similar bed of packed beads. The utility of this support was demonstrated by immobilizing α-glucoamylase by silane-glutaraldehyde coupling, and measuring its activity toward converting maltose to glucose. Using optimized immobilization conditions, an enzyme loading of 1.5 mg protein perm 2 surface area was obtained, with an activity of 370 units/g glass at 50°C. The half-life of the immobilized glucoamylase was more than twice as long as that of the free enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01020823
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  • 8
    Electronic Resource
    Electronic Resource
    Ethanol production from corn cob pretreated by the ammonia steeping process using genetically engineered yeast (1996)
    Springer
    Biotechnology letters 18 (1996), S. 1013-1018 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A new and effective pretreatment process for biomass conversion involves the steeping of biomass in 2.9 M NH4OH. This resulted in the removing about 80–90% of the lignin along with almost all the acetate from cellulosic residues. Based on dry cellulose from corn cob, a high glucose yield of 92% was obtained after enzymatic saccharification of cellulose fraction. By using a genetically engineered, xylosefermenting Saccharomyces 1400(pLNH33) in the batch fermentation of a glucose-xylose mixture from corn cob, an ethanol concentration of 47 g/L was obtained within 36 h with 84% yield. In addition, an ethanol concentration of 45 g/L was obtained within 48 h with 86% yield using simultaneous saccharification-fermentation process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00129723
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  • 9
    Electronic Resource
    Electronic Resource
    Hydrodynamic effect of surfactants on gas-liquid oxygen transfer (1980)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 26 (1980), S. 1008-1012 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An experimental investigation of the hydrodynamics at the surface region of the liquid reveals that the soluble surfactants (sodium lauryl sulfate, glucose oxidase, and bovine serum albumin) affect both the length and the velocity scales of liquid eddies approaching the surface, which results in a decrease in mass transfer.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690260616
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  • 10
    Electronic Resource
    Electronic Resource
    Glucose isomerase immobolized on porous glass (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 389-413 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Partially purified glucose isomerase from a Streptomyces species was immobilized on porous glass particles and studied for various characteristics concerning its use as an industrial catalyst. The activities were investigated in relation to the reaction parameters and the enzyme deactivation was studied systematically under various reaction conditions. The half-life of the immobilized enzyme was found to exceed 200 days at 50°C. The rate equation of the reversible glucose ⇄ fructose reaction was derived and the kinetic constants were determined. The rate equation was found to be in good agreement with experimental data for both forward and reverse reactions. The degree of diffusional effects was experimentally measured and theoretically analyzed.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260180309
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