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  • 1
    Electronic Resource
    Electronic Resource
    Correspondence. Thermal Conductivity of Two-Phase Materials (1962)
    s.l. : American Chemical Society
    Industrial and engineering chemistry 1 (1962), S. 222-223 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/i160003a013
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  • 2
    Electronic Resource
    Electronic Resource
    Egg White Lysozyme Purification by Ultrafiltration and Affinity Chromatography (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 58 (1993), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Isolation and purification of lysozyme from hen egg white was studied using a two-step procedure. The egg white was diluted 5- to 9-fold with sodium phosphate buffer, and then processed by sequential dilution diafiltration using a UF membrane (molecular weight cut-off 300,000 dalton). The membrane process increased the specific activity of lysozyme 6-fold, and recovered 96% of lysozyme activity. The permeate from diafiltration was further purified by affinity chromatography using chitin as adsorbent. The second step of the process yielded a product of specific activity of 70,400 units/mg protein. The overall lysozyme recovery was 79%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1993.tb04261.x
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  • 3
    Electronic Resource
    Electronic Resource
    Monitoring and control of methanol concentration during polysaccharide fermentation using an on-line methanol sensor (1992)
    Springer
    Bioprocess and biosystems engineering 7 (1992), S. 241-247 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Combining principles of membrane separation and semiconductor gas sensor technology, we constructed a methanol sensor to follow methanol concentrations on-line. A length of silicone tubing allowed for mass transfer of methanol from the fermentation medium to a carrier gas which then flowed over a semiconductor gas sensor for detection. The sterilizable sensor demonstrated excellent ability in following methanol concentrations during the batch production of a polysaccharide by the organism Methylomonas mucosa, even as the fermentation broth became increasingly viscous. During fed-batch control by feeding methanol to the fermentation to maintain setpoint methanol levels, a drift in the sensor signal was noted and quantified. A drift factor was determined which, after it was incorporated into the calibration calculations, improved methanol concentration control greatly. Methanol concentration was held constant over a range of set point concentrations during fedbatch fermentations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386233
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  • 4
    Electronic Resource
    Electronic Resource
    System development for size exclusion chromatography of starch hydrolysates (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 27 (1982), S. 2281-2293 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A system for the aqueous size exclusion chromatography (SEC) of starch hydrolysates has been developed. Sepharose CL is the chromatographic support material, and aqueous sodium hydroxide solution is the eluent. Various factors affecting the resolution of the proposed system are discussed. Support materials having small particle size and narrow particle size distribution are necessary for maximizing separation efficiency. The ionic strength of the eluent, however, has a negligible effect on separation efficiency. The fractionation range of the system has been broadened by connecting in series columns containing two different Sepharose-CL gel types according to the bimodal pore size distribution concept. Well-characterized sodium polystyrene sulfonate and dextran standards are used to calibrate this SEC system. The Coll-Prusinowski calibration procedure leads to a linear calibration curve over a wide molecular weight domain.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1982.070270636
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  • 5
    Electronic Resource
    Electronic Resource
    Polyelectrolytes as secondary calibration standards for aqueous size-exclusion chromatography (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 27 (1982), S. 795-810 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The effect of the ionic strength of the solvent on the size-exclusion chromatography of narrow molecular weight dextrans and monodisperse sodium polystyrene sulfonates has been investigated. In both NaOH and NaCl solutions the elution volume of the sodium polystyrene sulfonates increased as the ionic strength was increased, while the elution volume of the dextrans decreased slightly as the ionic strength was increased. The elution data for various ionic strength solvents could not be described by the traditional universal calibration procedure of plotting the product of the intrinsic viscosity and the molecular weight vs. the elution volume. By using a modified calibration procedure which includes excluded volume effects, the elution data can be described for NaOH solution of moderate and high ionic strength. This modified calibration technique was unable to describe the elution data for very low ionic strength NaOH solutions and for the NaCl solutions. Possible explanations of the inability of straightforward size exclusion chromatographic calibration techniques to quantitatively described all the observed elution behavior are discussed.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1982.070270302
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  • 6
    Electronic Resource
    Electronic Resource
    A simple hobby Computer-based off-gas analysis system (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 23 (1981), S. 455-460 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260230222
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  • 7
    Electronic Resource
    Electronic Resource
    Membrane reactor for enzymatic hydrolysis of cellobiose (1981)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 23 (1981), S. 1501-1516 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A pressurized, stirred vessel attached with an ultrafiltration membrane was used as a membrane reactor, Cellobiose hydrolysis by cellobiase was carried out and theoretically analyzed in terms of steady-state conversion and flow rate through the membrane. When the flow rate exceeds a critical value, a significant fraction of the enzyme inside the reactor is localized in the concentration polarization layer where shear from stirring is high. Consequently, enzyme deactivation inside the concentration polarization layer is accelerated and the conversion decreases due to an exchange of active enzyme in bulk with deactivated enzyme in the polarization layer via convection and back diffusion. Successful operation can be obtained at flow rates lower than the critical point to avoid the polarization and thus the deactivation. It is shown that 6.5 L of 2 mg/mL of cellobiose solution is hydrolyzed to glucose with a conversion of 91% in 20 h with 1.617 mg of cellobiase enzyme, in a reactor attached with a PM 10 membrane of an effective surface area of 39.2 cm2.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260230710
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of 2-keto-gulonic acid and fermentation substrates by HPLC (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 374-376 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260413
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  • 9
    Electronic Resource
    Electronic Resource
    Preparation of cellodextrins using sulfuric acid (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 557-559 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260260525
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  • 10
    Electronic Resource
    Electronic Resource
    Adsorption of ethanol-water mixtures by biomass materials (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 725-730 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260240314
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