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  • 1
    Electronic Resource
    Electronic Resource
    The pilA gene of Xanthomonas campestris pv. citri is required for infection by the filamentous phage cf (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 22-26 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsXanthomonas campestris pv. citri ; Bacterio- phage cf ; Pilin ; pilA ; Infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Host factors that are important for infection of Xanthomonas campestris pv. citri by the filamentous bacteriophage cf were investigated by transposon mutagenesis with Tn5tac1. A mutant, XT501, that was resistant to cf infection was recovered, showing that the gene inactivated by the transposon is required for infection by the phage but not for cf replication or assembly. A 1.7-kb SacI-ApaI DNA fragment from XT501 containing the bacterial DNA flanking one end of the transposon was cloned and shown to be required for cf infection. Nucleotide sequence analysis of the 1.7-kb fragment reveals the presence of an ORF that encodes a protein of 146 amino acids. This protein shows 42% identity to the type 4 prepilin encoded by the pilA genes of other bacteria. The pilA gene of X. campestris pv. citri is thus essential for infection by the bacteriophage cf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051055
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Radionecrosis of the temporal lobe: dynamic susceptibility contrast MRI (2000)
    Springer
    Neuroradiology 42 (2000), S. 149-152 
    Details
    ISSN: 1432-1920
    Keywords: Key words Carcinoma nasopharyngeal ; Radionecrosis ; Magnetic resonance imaging ; Pulse sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We examined nine patients with histologically proven nasopharyngeal carcinoma (NPC), treated with radiotherapy, using dynamic susceptibility contrast MRI (DSC-MRI). In eight there was clinical evidence of radionecrosis of the temporal lobe, and one was examined for local recurrence in the nasopharynx. All patients had either high-signal finger-like or cystic lesions in the temporal lobes on T2-weighted images. Heterogeneous contrast enhancement occurred in all patients. Relative regional cerebral blood volume (rrCBV) mapping revealed marked hypoperfusion in all patients. One underwent bilateral temporal lobectomy and radionecrosis was confirmed histologically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002340050036
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    Paper (German National Licenses)
    Fulltext
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