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  • 1
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 53-57 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Millet amylase formed during malting was used for teh saccharification of cassava (Manihot esculenta) into glucose syrup. The resulting syrup was also fermented to ethanol and citric acid. The maximum α-amylase activity occurred within 30 hours of millet germination at an optimum pH 8.0 and 40°C. The T. reesei enzymes enhanced the rate of cassava hydrolysis and paper chromatography revealed the presence of maltose, glucose and galactose in the cassava hydrolysate. The low cost of millet coupled with the fact that it is readily available makes it an economic source of amylase.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 11 (1991), S. 31-37 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Three yeast strains were isolated from a spontaneously fermented native millet (Pennisetum typhoideum) malt beer (Oyokpo). One of the yeast isolates found to have the most highly fermenting capacity was characterised and identified as a strain of Saccharomyces cerevisiae. The yeast was then utilised as the pitching yeast in a subsequent controlled fermentation of millet wort at 20°C for 120 hours. Bitter leaf (Vernonia amagdalina) extract was used as the bittering and flavouring agent. The Oyokpo beer sample produced under these conditions was found to possess both chemical and organoleptic qualities comparable to some extent, to the conventional barley malt beer. At the end of fermentation, the pH, specific gravity, alcohol content, reducing sugar content and protein content of the beer were 4.11, 1.0308, 2.81% (v/v), 4.00 (mg/ml) and 0.84 (mg/ml) respectively.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 8 (1988), S. 295-298 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A laboratory version of orgoro; a low alcohol plantain beverage was found to possess a better odour, taste and colour after 7 days of fermentation compared with a native brew. The pH of the native brew decreased from 3.90 to 1.93 after 21 days of fermentation while the filterable acidity increased from 0.100 to 0.157%. The laboratory brew showed a decrease of pH from 4.60 to 2.72 and an increase in filterable acidity from 0.250 to 0.485%. Both brews had alcohol contents of 0.109 and 0.197% (v/v) respectively after this period. The total sugars content, soluble protein and total nitrogen contents of the native brew were 2.0 mg/ml, 1.3 mg/ml and 2.05 mg/ml respectively while values for the laboratory brew were 5.0 mg/ml, 2.2 mg/ml and 4.0 mg/ml respectively.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 1 (1981), S. 153-159 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The improved method for preparing Oyokpo a Nigerian fermented beverage from millet, and the preparation of single cell proteins from the spent grain is described. Improvement of the brew was made by controlled malting, mashing and brewing with a pure culture of Saccharomyces cerevisiae. It had a reducing sugar content of 19.73 g/100 ml before fermentation and after fermentation 5.56% alcohol, 0.58 g/100 ml titratable acidity as acetic acid, a final pH of 4.2 and consisted of a yellowish clear liquid, slightly sour. The native brew had a reducing sugar content of 7.37 g/100 ml before fermentation and after fermentation, 2.40% alcohol, 0.43 g/100 ml titratable acidity, a final pH of 3.8 and consisted of a creamy yellowish liquid with a very sour taste. Fermented spent grain gave a higher protein yield compared to unfermented or ground millet. The lipids, proteins and crude fibre were 4.94%, 11.20% and 4.33% respectively for ground millet, 12,79%, 23.77% and 19.46% respectively for unfermented spent grain and 19.61%, 47.28% and 32.09% respectively for fermented spent grain. The high protein and fibre content of the fermented spent grain points to its potential as a feed supplement for ruminants.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 8 (1988), S. 39-45 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Investigations were conducted into the potential use of enzyme hydrolysed cassava whey for ethanol production by Saccharomyces cerevisiae Aspergillus niger grown on whct bran was used as crude enzyme source to saccharify the whey starch. The whey with an initial HCN concentration of 54.0μg/ml was fermented at pH 4.5 and 30°C in a one-step process to produce ethanol. A maximum ethanol concentration of 4.5% (v/v) was obtained in 120 h with a decrease in HCN level to 4.0 μg/ml. In a two-stage fermentation, in which the raw whey was pre-hydrolysed and under the same fermentation conditions, the unsterilized hydrolysate yielded alcohol content of 5.5% (v/v), while the sterilized hydrolysate gave higher alcohol yield, 7.5% (v/v), in 48 h. No HCN was detected in the fermented liquour at the end of the two-stage process.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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