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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 67 (1945), S. 691-692 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 143 (1995), S. 89-102 
    ISSN: 1432-1424
    Keywords: Drug resistance ; ATPases ; Chemotherapy ; Cancer ; Membrane Transport ; Xenobiotics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Conclusions The initial discovery of p-glycoprotein in the plasma membrane of MDR cancer cell lines was followed quickly by the cloning of its gene. Sequence analysis of cloned cDNAs revealed that p-glycoprotein was a member of the ABC family of membrane transporters. Subsequent biochemical characterization demonstrated the binding of chemotherapeutic drugs and ATP to p-glycoprotein. P-glycoprotein-mediated drag transport and drug-stimulated ATPase activity were documented in plasma membrane vesicles and in proteoliposomes containing the partially purified protein. P-glycoprotein was shown to be phosphorylated and the effect of this modification on the protein's biological function was explored. P-glycoproteins were found in many normal tissues and their overexpression was documented in numerous cancers. An important role for p-glycoprotein in intrinsic and acquired drug resistance in clinical oncology was established. Despite all that has been learned about p-glycoprotein over the last few years, additional studies will be necessary to address the many questions that have been left unanswered. Determination of p-glycoprotein structure and membrane topology should help elucidate the nature of chemotherapeutic drug binding sites and the mechanism whereby drug movement is coupled to ATP hydrolysis. Complete purification and functional reconstitution of p-glycoprotein into defined lipid vesicles will permit further characterization of drug transport and ATPase activity and give us the means by which p-glycoprotein's apparent dual function as a transporter and a channel can be clarified. Structural and functional studies on p-glycoprotein will also provide information needed to develop specified inhibitors that can be used clinically to overcome MDR in cancer patients. Further study of the mechanisms whereby p-glycoprotein expression is induced and regulated during malignant transformation is indicated. The development of biliary phospholipid deficiency in mdr2 knockout mice and xenobiotic hypersensitivity in mdr3 knockout mice have given us the first clues into the normal physiologic roles for the p-glycoproteins. The search for endogenous substrates for the p-glycoproteins will continue to be an area of active investigation. Continued investigation of p-glycoprotein's functions should result in better understanding of an important class of prokaryotic and eukaryotic membrane transporters. The potential of exploiting the knowledge garnered from these studies in the treatment of neoplastic, parasitic and inherited and acquired liver disease may be greater than we can now imagine.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0789
    Keywords: Key words Dissolved organic nitrogen ; Soluble organic nitrogen ; Nitrogen transformations ; Nitrogen loss ; Leaching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The existence of soluble organic forms of N in rain and drainage waters has been known for many years, but these have not been generally regarded as significant pools of N in agricultural soils. We review the size and function of both soluble organic N extracted from soils (SON) and dissolved organic N present in soil solution and drainage waters (DON) in arable agricultural soils. SON is of the same order of magnitude as mineral N and of equal size in many cases; 20–30 kg SON-N ha–1 is present in a wide range of arable agricultural soils from England. Its dynamics are affected by mineralisation, immobilisation, leaching and plant uptake in the same way as those of mineral N, but its pool size is more constant than that of mineral N. DON can be sampled from soil solution using suction cups and collected in drainage waters. Significant amounts of DON are leached, but this comprises only about one-tenth of the SON extracted from the same soil. Leached DON may take with it nutrients, chelated or complexed metals and pesticides. SON/DON is clearly an important pool in N transformations and plant uptake, but there are still many gaps in our understanding.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 19 (1992), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A 57-year-old woman with cutaneous mastocytosis of 23 years duration developed a hyperpigmented abdominal plaque composed of confluent indurated papules that enlarged for a period of 1 year to 12×8 cm. Biopsy showed dermal infiltration by closely packed spindle-shaped mast cells, fibroblasts, collagen, and scattered lymphocytes, predominately T-suppressor cells/ Electron microscopy showed close contact between mast cells, fibroblasts, and lymphocytes Piecemeal mast cell degranulation and extrusion of mast cell granules was seen, with rare mast cell granules in fibroblasts, and collagen fibers in peripheral and perinuclear endoplasmic reticulum of mast cells. The term Fibrous mastocytoma is suggested for this tumor-like dermal fibrosis, possibly induced by lymphokines.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 197 (1963), S. 226-228 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE year 1962 was the tercentenary of the publication of one of the best-known laws of science, Boyle's law, which stated that the volume of an enclosed gas was inversely proportional to the pressure applied to it1. As originally verified, the law only applied to air, which was at that time not ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Gene 59 (1987), S. 253-263 
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; deletion analysis ; divergent promoter ; melibiose-dependent transcription ; nucleotide sequence analysis ; positive control of transcription ; transcript mapping
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0378-1119
    Keywords: C terminus deletions ; DNase footprinting ; MeIR binding sequences ; Recombinant DNA ; amino acid sequence homologies ; gel retardation assays ; melR-lacZ fusions
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Gene 68 (1988), S. 297-305 
    ISSN: 0378-1119
    Keywords: CRP binding site ; DNase footprinting ; Recombinant DNA ; deletion analysis ; melAB promoter activity ; nucleotide sequence homologies ; transcript mapping
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Previous studies with purified variants of the 434 repressor having different operator-binding specificities have demonstrated the interactions of a heterodimeric repressor with a hybrid operator site. The present study investigates the interactions between the 434 repressor and its operator site. The optimum 434 operator half-site is used with a P22 operator half-site to create a hybrid 434/P22 operator. We show that this hybrid operator can be efficiently bound by a heterodimeric repressor, consisting of one wild-type 434 repressor monomer and one 434 repressor monomer with the binding specificity of the P22 repressor, to bring about repression in Escherichia coli.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 229 (1971), S. 463-463 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Although Britain is only now adopting a decimal currency, the idea was first mooted three hundred years ...
    Type of Medium: Electronic Resource
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