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  • 1
    Electronic Resource
    Electronic Resource
    Glucose entry into rat mesangial cells is mediated by both Na+-coupled and facilitative transporters (1995)
    Springer
    Diabetologia 38 (1995), S. 291-297 
    Details
    ISSN: 1432-0428
    Keywords: Key words Na+-coupled glucose transporter ; facilitative glucose transporters ; mesangial cells ; glomerulopathy ; phlorizin.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Since previous studies from our laboratory have demonstrated that increased glucose consumption by cultured rat mesangial cells is accompanied by an accelerated production of type IV and type VI collagen, we have now examined the manner by which glucose is transported into these cells. A progressive stimulation of glucose uptake by the mesangial cells was observed with increasing concentrations of NaCl so that at 145 mmol/l about twice as much glucose entered the cells as in its absence (substituted by choline chloride). Moreover, since phlorizin inhibited the NaCl-promoted uptake of glucose and this salt was found to increase the accumulation of α-methylglucoside in a manner which could not be duplicated by KCl or mannitol, both Na+-coupled and facilitative glucose transporters appeared to be present in the cells. Km values of 1.93 mmol/l and 1.36 mmol/l were determined for the co-transport and facilitated transport pathways, respectively, with their Vmax being 29.5 and 18.0 nmol · mg protein− 1· h− 1. Both uptake activities were found to be down-regulated by exposure of the cells to high glucose and furthermore the Na+-dependent transport could no longer be detected after about 12 passages of the cells. Hybridization of mesangial cell mRNA with cDNA probes revealed transcripts for the Na+/glucose co-transporter as well as GLUT1 and to a lesser extent GLUT4. The identification of the co-transporter in these non-polarized cells is pertinent to an understanding of the intracellular signals which can lead to the development of the diabetic glomerular lesions; in the hyperglycaemic state this carrier provides an additional route for accelerated glucose entry and furthermore by the attendant increase in Na+ flux may bring about an alteration in the ionic composition of the cell. [Diabetologia (1995) 38: 291–297]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400633
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  • 2
    Electronic Resource
    Electronic Resource
    Glucose entry into rat mesangial cells is mediated by both Na+-coupled and facilitative transporters (1995)
    Springer
    Diabetologia 38 (1995), S. 291-297 
    Details
    ISSN: 1432-0428
    Keywords: Na+-coupled glucose transporter ; facilitative glucose transporters ; mesangial cells ; glomerulopathy ; phlorizin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Since previous studies from our laboratory have demonstrated that increased glucose consumption by cultured rat mesangial cells is accompanied by an accelerated production of type IV and type VI collagen, we have now examined the manner by which glucose is transported into these cells. A progressive stimulation of glucose uptake by the mesangial cells was observed with increasing concentrations of NaCl so that at 145 mmol/l about twice as much glucose entered the cells as in its absence (substituted by choline chloride). Moreover, since phlorizin inhibited the NaCl-promoted uptake of glucose and this salt was found to increase the accumulation of α-methylglucoside in a manner which could not be duplicated by KCl or mannitol, both Na+-coupled and facilitative glucose transporters appeared to be present in the cells. Km values of 1.93 mmol/l and 1.36 mmol/l were determined for the co-transport and facilitated transport pathways, respectively, with their Vmax being 29.5 and 18.0 nmol·mg protein−1· h−1. Both uptake activities were found to be down-regulated by exposure of the cells to high glucose and furthermore the Na+-dependent transport could no longer be detected after about 12 passages of the cells. Hybridization of mesangial cell mRNA with cDNA probes revealed transcripts for the Na+/glucose co-transporter as well as GLUT1 and to a lesser extent GLUT4. The identification of the co-transporter in these non-polarized cells is pertinent to an understanding of the intracellular signals which can lead to the development of the diabetic glomerular lesions; in the hyperglycaemic state this carrier provides an additional route for accelerated glucose entry and furthermore by the attendant increase in Na+ flux may bring about an alteration in the ionic composition of the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400633
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  • 3
    Electronic Resource
    Electronic Resource
    Septic thrombosis of the portal vein due to peripancreatic ligamental abscess (1999)
    Springer
    European radiology 9 (1999), S. 90-92 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Portal vein thrombosis ; Septic thrombosis ; Peripancreatic abscess ; Peritoneal ligaments ; Computed tomography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Septic thrombus formation of both the main portal vein and its intrahepatic branches were observed on CT in a patient with peripancreatic abscess. The septic thrombosis of portal vein (STPV) extended from the level of porta hepatis into the intrahepatic branches, but the portal vein and superior mesenteric vein at the level of pancreatic head were preserved with no evidence of thrombosis angiographically. The gas-containing abscess near the head of the pancreas extended toward the hepatic hilum and surrounded the portal vein and its branches on CT. It was concluded that these thrombi of portal vein branches at porta hepatis and intrahepatic branches were caused by extensions of peripancreatic abscess via the hepatoduodenal ligament and ligamentum teres. Computed tomography was useful in depicting the ligamentous spread of peripancreatic abscess resulting in STPV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050634
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  • 4
    Electronic Resource
    Electronic Resource
    Probucol reduces lysophosphatidylcholines in low-density lipoprotein (2000)
    Springer
    European journal of clinical pharmacology 55 (2000), S. 787-792 
    Details
    ISSN: 1432-1041
    Keywords: Key words Probucol ; Lysophosphatidylcholine ; Low-density lipoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Objectives: Lysophosphatidylcholine (LPC) in low-density lipoprotein (LDL) comes into notice as an important atherogenic substance. Methods: Since the effect of probucol, an antioxidative lipid-lowering drug, on LPC molecular species has not been elucidated, two LPC molecular species, stearoyl LPC (SLPC) and palmitoyl LPC (PLPC), were measured in LDL using high-pressure liquid chromatography. LDL was obtained from 11 hyperlipidemic patients, including 9 diabetic patients, in comparison with 11 age- and gender-matched controls. Results and conclusion: Hyperlipidemic patients showed nearly twofold higher levels of SLPC and PLPC per gram of LDL protein than those of controls. All hyperlipidemic patients were treated with oral administration of 500 mg/day of probucol for 3 months. Both LPCs in LDL were significantly reduced to control levels and were increased again up to the pretreatment levels 4 weeks after cessation of the treatment. Therefore, probucol has a potent effect in reducing LPC and may contribute to decreasing the atherogenicity of LDL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002280050698
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  • 5
    Electronic Resource
    Electronic Resource
    Reaction profiles of seven enzyme immunoassay kits for carcinoembryonic antigen (CEA) analyzed with purified preparations of CEA and related normal antigens
    Amsterdam : Elsevier
    Clinical Biochemistry 25 (1992), S. 29-35 
    Details
    ISSN: 0009-9120
    Keywords: CEA-related normal antigens ; carcinoembryonic antigen ; commercial kits ; enzyme immunoassay ; monoclonal antibody
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0009-9120(92)80042-F
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  • 6
    Electronic Resource
    Electronic Resource
    Renal salvage procedure for synchronous bilateral Wilms' tumor (2000)
    Springer
    Pediatric surgery international 16 (2000), S. 222-225 
    Details
    ISSN: 1437-9813
    Keywords: Key words Bilateral Wilms' tumor ; Operation ; Renal salvage technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report describes a renal salvage procedure performed in a 2-year-old girl with bilateral renal tumors comprising a multilocular, cystic tumor of the right kidney and a solid and cystic nephroblastoma of the left kidney after chemotherapy. Surgery was performed because the right kidney became hydronephrotic due to compression by the enlarged cysts, while the left tumor showed only minimal shrinkage even after three courses of chemotherapy. The right-sided cysts were simply unroofed and the left-sided tumor was extirpated by partial nephrectomy. Her postoperative course was uneventful with considerable recovery of the function of each kidney. This procedure should be considered if the pathological features of the tumor are relatively favourable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003830050730
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  • 7
    Electronic Resource
    Electronic Resource
    Recurrent angiosarcoma effectively treated with superselective continuous intra-arterial injection of recombinant interleukin-2 combined with radiotherapy (1998)
    Springer
    International journal of clinical oncology 3 (1998), S. 396-399 
    Details
    ISSN: 1437-7772
    Keywords: Angiosarcoma ; Interleukin-2 ; Intra-arterial injection ; Radiotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a case of recurrent angiosarcoma (AS) in a 63-year-old man, effectively treated with a superselective continuous intra-arterial injection of recombinant interleukin-2 (rIL-2) combined with electron beam radiotherapy. The patient, who had undergone surgical resection for AS on his face near the nasal bridge, was admitted to our hospital because of recurrent AS on his bilateral cheeks. We placed 4-French catheters in the right external carotid artery (ECA) and superselectively in the left facial artery. A continuous intra-arterial injection of rIL-2 was administered in combination with radiotherapy using a 6 MeV electron beam. The lesion rapidly decreased in size. The total dose of rIL-2 was 3.72 × 107 IU (International Unit) in the right ECA and 2.96 × 107 IU in the left facial artery, while the total radiation dose was 50 Gy, in 25 fractions, for the tumor on the right cheek and 46 Gy, in 23 fractions, for the erythematous plaque on the left cheek. The patient was discharged with almost no cutaneous lesion on his cheeks. Unfortunately, there was another recurrence of AS and the patient died of respiratory failure due to lung metastases. However, as the lesion could be completely controlled even for a while, we believe this treatment method will contribute to the future management of AS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00539220
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  • 8
    Electronic Resource
    Electronic Resource
    Demonstration of apoptosis in neuroblastoma and its relationship to tumour regression (1995)
    Springer
    Virchows Archiv 427 (1995), S. 167-173 
    Details
    ISSN: 1432-2307
    Keywords: Neuroblastoma ; Apoptosis ; bcl-2 ; Fas antigen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The in vivo occurrence of apoptosis in neuroblastomas was investigated. Histologically, a number of tumour cells showed typical apoptotic changes, including cell shrinkage, condensed and fragmented nuclei, eosinophilic cytoplasm, and absence of the inflammatory response. These cells coincided closely with the so-called karyorrhectic cells. An electrophoretic DNA ladder, a functional hallmark of apoptosis, was demonstrated in four of six tumours, and DNA fragmentation was detected in situ by terminal deoxytransferase-mediated nick end-labelling in 26 of 35 tumour specimens (74%). The labelled cell counts ranged from 5 to 62 per 5000 tumour cells (mean±SD: 15.0±14.5). Immunoperoxidase staining revealed that an apoptosis-suppressing protein, bcl-2, was expressed abundantly in advanced-stage tumours, whereas it was absent from karyorrhectic-apoptotic cells. Several tumours with the potential for spontaneous regression were bcl-2-deficient. Immunostaining of the Fas receptor for apoptosis demonstrated that the tumour cells expressed this molecule on their cell surfaces. Our results provide evidence of apoptosis in neuroblastomas and suggest that bcl-2 and the Fas receptor may play a role in its regulatory mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196522
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  • 9
    Electronic Resource
    Electronic Resource
    A new model of Type 2 (non-insulin-dependent) diabetes mellitus in spontaneously hypertensive rats: diabetes induced by neonatal streptozotocin treatment (1986)
    Springer
    Diabetologia 29 (1986), S. 808-811 
    Details
    ISSN: 1432-0428
    Keywords: Hypertension ; streptozotocin ; animal model ; spontaneously hypertensive rats ; Type 2 (non-insulin-dependent) diabetes mellitus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This study was designed to develop an animal model of Type 2 (non-insulin-dependent) diabetes with persistent hypertension. Male spontaneously hypertensive rats were treated with 25.0, 37.5, 50.0, 62.5 or 75.0 mg/kg of streptozotocin given intraperitoneally at 2 days of age and maintained for 12 weeks. In the rats which received 50.0 mg/kg or more streptozotocin, overt hyperglycaemia gradually and consistently developed following incomplete recovery from an initial hyperglycaemia. Compared to vehicle-treated controls, body weight gain in these animals did not differ for the first 8 weeks; thereafter, it was slightly but significantly (p 〈 0.05) reduced. The animals treated with 25.0 or 37.5 mg/kg streptozotocin developed mild to moderate hyperglycaemia, but their body weight gain was similar to controls. The relationships between streptozotocin dose and metabolic responses (plasma glucose, glycosylated haemoglobin, urinary glucose, food intake, etc.) were clearly demonstrated. Systolic blood pressure rose with progressing age in both controls and streptozotocin-treated rats, irrespective of dosage or metabolic response. This new rat model of Type 2 diabetes associated with persistent hypertension may be useful in studying these combined effects on small and large vessels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00873221
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  • 10
    Electronic Resource
    Electronic Resource
    Early development of nephropathy in a new model of spontaneously hypertensive rat with non-insulin-dependent diabetes mellitus (1988)
    Springer
    Diabetologia 31 (1988), S. 291-296 
    Details
    ISSN: 1432-0428
    Keywords: Non-insulin-dependent diabetes mellitus ; hypertension ; nephropathy ; urinary protein ; streptozotocin ; N-acetyl-β-D-glucosaminidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We designed the present study to clarify whether the development of nephropathy was accelerated by a combination of hypertension and non-insulin-dependent diabetes. Spontaneously hypertensive rats with non-insulin-dependent diabetes induced by neonatal streptozotocin treatment (25.0–75.0 mg/kg) were separated into severely or mildly diabetic groups according to their non-fasting plasma glucose levels at 12 weeks of age and the findings were compared with the data on a control group treated with citrate buffer alone. The natural courses of urinary excretion rate of total protein, the molecular composition by sodium dodecyl sulfate polyacrylamide gel electrophoresis with laser desitometer and N-acetyl-β-D-glucosaminidase were measured in the three groups from 12 weeks until 36 weeks of age. Total urinary protein in the control group decreased with age (p〈0.05), while in the mildly diabetic group changes were nil; in the severely diabetic group, however, the excretion rates of total urinary protein and high molecular weight protein consistently and progressively increased with age (p〈0.05). The low molecular weight protein continuously decreased with age in the mildly diabetic and control groups (p〈0.05), while in the severely diabetic group there was no decrease after 28 weeks of age. The urinary N-acetyl-β-D-glucosaminidase markedly increased (p〈0.05) in the severely diabetic group throughout the period compared with findings in the control group, but drastically decreased (p〈0.05) in the mildly diabetic group with age. There were significant correlations between the mean glycosylated haemoglobin levels and all the urinary parameters measured (p〈0.05). These observations suggest that development of nephropathy is accelerated by the glycaemic level in hypertensive rats. This new model should be appropriate for studying the combined effects of hypertension and diabetes mellitus on the kidney.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277410
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