Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Glucose entry into rat mesangial cells is mediated by both Na+-coupled and facilitative transporters (1995)
    Springer
    Diabetologia 38 (1995), S. 291-297 
    Details
    ISSN: 1432-0428
    Keywords: Key words Na+-coupled glucose transporter ; facilitative glucose transporters ; mesangial cells ; glomerulopathy ; phlorizin.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Since previous studies from our laboratory have demonstrated that increased glucose consumption by cultured rat mesangial cells is accompanied by an accelerated production of type IV and type VI collagen, we have now examined the manner by which glucose is transported into these cells. A progressive stimulation of glucose uptake by the mesangial cells was observed with increasing concentrations of NaCl so that at 145 mmol/l about twice as much glucose entered the cells as in its absence (substituted by choline chloride). Moreover, since phlorizin inhibited the NaCl-promoted uptake of glucose and this salt was found to increase the accumulation of α-methylglucoside in a manner which could not be duplicated by KCl or mannitol, both Na+-coupled and facilitative glucose transporters appeared to be present in the cells. Km values of 1.93 mmol/l and 1.36 mmol/l were determined for the co-transport and facilitated transport pathways, respectively, with their Vmax being 29.5 and 18.0 nmol · mg protein− 1· h− 1. Both uptake activities were found to be down-regulated by exposure of the cells to high glucose and furthermore the Na+-dependent transport could no longer be detected after about 12 passages of the cells. Hybridization of mesangial cell mRNA with cDNA probes revealed transcripts for the Na+/glucose co-transporter as well as GLUT1 and to a lesser extent GLUT4. The identification of the co-transporter in these non-polarized cells is pertinent to an understanding of the intracellular signals which can lead to the development of the diabetic glomerular lesions; in the hyperglycaemic state this carrier provides an additional route for accelerated glucose entry and furthermore by the attendant increase in Na+ flux may bring about an alteration in the ionic composition of the cell. [Diabetologia (1995) 38: 291–297]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400633
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Glucose entry into rat mesangial cells is mediated by both Na+-coupled and facilitative transporters (1995)
    Springer
    Diabetologia 38 (1995), S. 291-297 
    Details
    ISSN: 1432-0428
    Keywords: Na+-coupled glucose transporter ; facilitative glucose transporters ; mesangial cells ; glomerulopathy ; phlorizin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Since previous studies from our laboratory have demonstrated that increased glucose consumption by cultured rat mesangial cells is accompanied by an accelerated production of type IV and type VI collagen, we have now examined the manner by which glucose is transported into these cells. A progressive stimulation of glucose uptake by the mesangial cells was observed with increasing concentrations of NaCl so that at 145 mmol/l about twice as much glucose entered the cells as in its absence (substituted by choline chloride). Moreover, since phlorizin inhibited the NaCl-promoted uptake of glucose and this salt was found to increase the accumulation of α-methylglucoside in a manner which could not be duplicated by KCl or mannitol, both Na+-coupled and facilitative glucose transporters appeared to be present in the cells. Km values of 1.93 mmol/l and 1.36 mmol/l were determined for the co-transport and facilitated transport pathways, respectively, with their Vmax being 29.5 and 18.0 nmol·mg protein−1· h−1. Both uptake activities were found to be down-regulated by exposure of the cells to high glucose and furthermore the Na+-dependent transport could no longer be detected after about 12 passages of the cells. Hybridization of mesangial cell mRNA with cDNA probes revealed transcripts for the Na+/glucose co-transporter as well as GLUT1 and to a lesser extent GLUT4. The identification of the co-transporter in these non-polarized cells is pertinent to an understanding of the intracellular signals which can lead to the development of the diabetic glomerular lesions; in the hyperglycaemic state this carrier provides an additional route for accelerated glucose entry and furthermore by the attendant increase in Na+ flux may bring about an alteration in the ionic composition of the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400633
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Effect of high glucose on formation of extracellular matrix components by cultured rat heart endothelial cells (1995)
    Springer
    Diabetologia 38 (1995), S. 430-436 
    Details
    ISSN: 1432-0428
    Keywords: Rat heart endothelial cells ; type VI collagen ; type IV collagen ; type I collagen ; fibronectin ; GLUT1 ; high glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In an attempt to define the basis for the microvascular changes observed in diabetic myocardium, a study was undertaken on the effect of elevated glucose on the synthesis by rat heart endothelial cells of the extracellular matrix components, types VI, IV and I collagen, as well as fibronectin. Confluent cultures of these cells, isolated by fluorescence-activated cell sorting after treatment with rhodamine-labelled acetylated low density lipoprotein, showed a three to fivefold enhancement in the synthesis of type VI collagen after exposure for 48 h to high glucose (20 to 30 mmol/l), as determined by immunoblot analysis. Increased production of type IV collagen and fibronectin was also observed, but the change was smaller and no effect on type I collagen was found. Measurement of mRNA levels by hybridization with cDNA probes indicated that 48-h exposure to high glucose significantly increased the level of transcripts for type VI and IV collagens but not for type I collagen. While glucose consumption by endothelial cells in high glucose doubled in the initial 24-h period, utilization returned to normal by 48 h, concomitant with a reduction in GLUT1 transcript levels, suggesting that signals for stimulation of collagen synthesis must be active during the initial period of exposure to elevated glucose levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410280
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Effect of high glucose on formation of extracellular matrix components by cultured rat heart endothelial cells (1995)
    Springer
    Diabetologia 38 (1995), S. 430-436 
    Details
    ISSN: 1432-0428
    Keywords: Key words Rat heart endothelial cells ; type VI collagen ; type IV collagen ; type I collagen ; fibronectin ; GLUT1 ; high glucose.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In an attempt to define the basis for the microvascular changes observed in diabetic myocardium, a study was undertaken on the effect of elevated glucose on the synthesis by rat heart endothelial cells of the extracellular matrix components, types VI, IV and I collagen, as well as fibronectin. Confluent cultures of these cells, isolated by fluorescence-activated cell sorting after treatment with rhodamine-labelled acetylated low density lipoprotein, showed a three to fivefold enhancement in the synthesis of type VI collagen after exposure for 48 h to high glucose (20 to 30 mmol/l), as determined by immunoblot analysis. Increased production of type IV collagen and fibronectin was also observed, but the change was smaller and no effect on type I collagen was found. Measurement of mRNA levels by hybridization with cDNA probes indicated that 48-h exposure to high glucose significantly increased the level of transcripts for type VI and IV collagens but not for type I collagen. While glucose consumption by endothelial cells in high glucose doubled in the initial 24-h period, utilization returned to normal by 48 h, concomitant with a reduction in GLUT1 transcript levels, suggesting that signals for stimulation of collagen synthesis must be active during the initial period of exposure to elevated glucose levels. [Diabetologia (1995) 38: 430–436]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410280
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Late quaternary sediments in Lake Zürich, Switzerland (1983)
    Springer
    Environmental geology 5 (1983), S. 191-205 
    Details
    ISSN: 1432-0495
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Lake Zürich occupies a glacially overdeepened perialpine trough in the northern Middlelands of Switzerland. A total of 154.4 m of Quaternary sediments and 47.3 m of Tertiary Molasse bedrock has been cored from the deepest part of the lake, some 10 km south of the city of Zürich. Some 16.8 m of gravels and sands directly overlying the bedrock include basal till and probably earliest subglacial fluvial and lacustrine deposits. These are overlain by 98.6 m of fine-grained, glacial-aged sediments comprising completely deformed proglacial and/or subglacial lacustrine muds, separated by four basal mud tills. The lack of interglacial sediments, fossils, and other datable material, and the presence of severe sediment deformation and unknown amounts of erosion prevent the establishment of an exact chronostratigraphy for sediments older than the upper mud till. Above it some 8.6 m of lacustrine muds were deposited, folded, faulted, and tilted during the final opening of the lake at about 17,500–17,000 years ago. Superimposed are 30.4 m of final Würm and post-glacial sediments comprising (from oldest): cyclic proglacial mud, thick-bedded and laminated mud, a complex transition zone, laminated carbonate, laminated marl, and diatom-calcite varves. These sediments reflect changing catchment and lacustrine conditions including: glacial proximity, catchment stability, lake inflow characteristics, thermal structure, chemistry, and bed stability. Average sedimentation rates ranged from 11 cm yr−1 immediately after glacier withdrawal, to as low as 0.4 mm yr−1 as the environment stabilized. The lack of coarse outwash deposits separating the fine-grained glaciolacustrine sediments from a corresponding underlying basal till suggests that deglaciation of the deep northern basin of Lake Zürich was by stagnation-zone retreat rather than by retreat of an active ice-front.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02414864
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Local reactions and IgE antibodies to pertussis toxin after acellular diphtheria-tetanus-pertussis immunization (1999)
    Springer
    European journal of pediatrics 158 (1999), S. 989-994 
    Details
    ISSN: 1432-1076
    Keywords: Key words Pertussis ; Pertussis toxin ; Acellular DTP vaccine ; Diphtheria ; Tetanus ; AbbreviationsDTPa diphtheria tetanus acellular pertussis vaccine ; FHA filamentous haemagglutinin ; PRN pertactin ; PT pertussis toxin ; RAST radio-allergosorbent test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Local reactions and pertussis toxin specific immunoglobulin E antibodies (PT-IgE) were investigated in healthy children following primary and booster immunization with a combined diphtheria tetanus acellular pertussis vaccine (DTPa) including pertussis toxin, filamentous haemagglutinin and pertactin. A primary series of DTPa was administered to 150 infants, and 104 of them received a booster dose of DTPa combined with inactivated polio vaccine at 2 years of age. PT-IgE was measured in serum samples from 72 children using a modified nitrocellulose RAST. Primary immunization was associated with low incidence of local reactions (1%–5%). After the booster dose 21% of children had a local reaction ≥20 mm. Local reactions after the booster dose tended to be more common in children who had experienced reaction at primary immunization. PT-IgE was detected in 18% and 86% of children following primary and booster vaccinations, respectively. Allergic and non-allergic children did not differ in PT-IgE responses. After primary immunization, elevated PT-IgE levels were found more often in children with a family history of allergy than in those without known allergy in the family. Children with local reactions had significantly higher pre- and post-booster PT-IgE levels and median post-booster pertactin IgG and diphtheria-IgG levels than children without local reactions. Conclusion Acellular pertussis immunization induces IgE antibodies to pertussis toxin, especially after booster vaccination. The higher median pre- and post-booster levels of pertussis toxin specific immunoglobulin E and post-booster levels of IgG to pertactin and diphtheria in children with local side-effects reflect a multifactorial immunological mechanism of such reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004310051264
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Effects of thermocyclers and primers on the reproducibility of banding patterns in randomly amplified polymorphic DNA analysis
    Amsterdam : Elsevier
    Molecular and Cellular Probes 8 (1994), S. 155-160 
    Details
    ISSN: 0890-8508
    Keywords: PCR, RAPDA, DNA, thermocyclers, primers, Bordetella pertussis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/mcpr.1994.1021
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Studies of flux-pinning energies in high-J"c YBa"2Cu"3O"7 prepared by melt-textured growth method
    Amsterdam : Elsevier
    Physica C: Superconductivity and its applications 174 (1991), S. 355-358 
    Details
    ISSN: 0921-4534
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0921-4534(91)91569-P
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Scaling law and flux pinning in neutron-irradiated YBa"2Cu"3O"y crystal prepared by melt-textured growth method
    Amsterdam : Elsevier
    Physica C: Superconductivity and its applications 195 (1992), S. 286-290 
    Details
    ISSN: 0921-4534
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0921-4534(92)90352-D
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Scaling properties of the magnetization curves of the DyBa"2Cu"3O"y crystal prepared by the melt-textured growth method
    Amsterdam : Elsevier
    Physica C: Superconductivity and its applications 222 (1994), S. 267-270 
    Details
    ISSN: 0921-4534
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0921-4534(94)90542-8
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...