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  • 1
    Electronic Resource
    Electronic Resource
    Effects of fibroblast-mediated interleukin 3 and interleukin 6 gene therapy on hematopoiesis in mice treated with 5-fluorouracil (1998)
    Springer
    Journal of molecular medicine 76 (1998), S. 782-789 
    Details
    ISSN: 1432-1440
    Keywords: Key words Hematopoiesis ; Interleukin 3 ; Interleukin 6 ; Gene therapy ; Chemotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Fibroblast-mediated cytokine gene therapy has proven to be a promising strategy for restoring hematopoiesis following repeated chemotherapy. Interleukin 3 (IL-3) and interleukin 6 (IL-6) can synergistically promote the recovery of hematopoiesis following chemotherapy. In this investigation, combined use of fibroblast-mediated IL-3 and IL-6 gene therapy was tested for hematopoietic effects on mice with or without 5-fluorouracil administration. The results demonstrated that combined therapy with IL-3 gene-modified NIH3T3 cell (NIH3T3-IL-3) and IL-6 gene-modified fibroblast NIH3T3 cell (NIH3T3-IL-6) implantation achieves obvious stimulation of hematopoiesis in normal mice and accelerates recovery of hematopoiesis. In normal mice the quantities of platelets, neutrophils, and total white blood cells in peripheral blood increased significantly after the combined implantation of NIH3T3-IL-3 and NIH3T3-IL-6 cells. The numbers of colony-forming unit (CFU) granulocyte/macrophage (CFU-GM) and CFU megakaryocyte (CFU-MK) formed by stem cells in bone marrow was significantly higher after the combined implantation of NIH3T3-IL-3 and NIH3T3-IL-6 cells than after the implantation of NIH3T3-IL-3 alone, NIH3T3-IL-6 alone, or neomycin gene-modified NIH3T3 cells. In hematopoiesis-depressed mice induced by preinjection with 5-fluorouracil at the dose of 150 mg/kg before cell implantation, the platelets, neutrophils, and white blood cells showed accelerated recovery, and the numbers of CFU-GM and CFU-MK formed by bone marrow cells were also markedly higher after the combined implantation of NIH3T3-IL-3 and NIH3T3-IL-6 cells than in control groups. Our data show that combined use of fibroblast-mediated IL-3 and IL-6 gene therapy may be of clinical relevance for the recovery of hematopoietic depression for patients after chemotherapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001090050280
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  • 2
    Electronic Resource
    Electronic Resource
    A study on the exchange interaction in R2Fe17 compounds (1996)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 79 (1996), S. 7883-7886 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A study on the exchange interaction in R2Fe17 compounds (R represents the heavy rare earth elements) has been performed by means of a mean-field analysis of the high-field magnetization curves, which were measured on the powder samples. Measurements have been carried out on R2Fe17-based quasiternary R2Fe17−xMx compounds with R=Dy, Ho, and Er, M=Al, Ga, and Si. It has been found that the value of the exchange coupling constant decreases with increasing atomic number of R ion of the compound. This behavior is explained by the varying 4f–5d hybridization in the compounds. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.362399
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  • 3
    Electronic Resource
    Electronic Resource
    Magnetocrystalline anisotropy of TbFe12−xTix single crystals (2000)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 76 (2000), S. 1170-1172 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: TbFe12−xTix single crystals have been investigated by means of magnetic measurements. With decreasing temperature, a spin-reorientation transition from easy axis to easy plane occurs. The rate of decrease of spin-reorientation temperature with x is about −380 K/Ti. There exists a strong magnetocrystalline anisotropy in the basal plane. The anisotropy field Ba between [100] and [110] for the compound TbFe11.15Ti0.85 is as high as 35 T which is about three times Ba in the axial direction. With increasing x, the easy magnetization direction at low temperatures in the basal plane changes from [110] for x=0.85 to [100] for x=1.0. The composition dependence of anisotropy can be understood in terms of a change of the crystal field parameters Anm due to substitution of Ti for Fe. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.125973
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  • 4
    Electronic Resource
    Electronic Resource
    Structure and magnetic properties of Gd3(Fe1−xCox)25Cr4 compounds (1999)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 74 (1999), S. 4020-4022 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The structural and magnetic properties of Gd3(Fe1−xCox)25Cr4 compounds with x=0–0.6 have been investigated. The structures of all the compounds investigated are found to possess monoclinic symmetry and belong to the A2/m space group. Substitution of Co for Fe leads to a clear contraction of the unit-cell volume. The Curie temperature increases from 492 K for x=0 to 762 K for x=0.6. The composition dependence of the saturation magnetization at 5 K reaches a maximum around x=0.3. It is noteworthy that substitution of Co for Fe results in a significant change of the magnetocrystalline anisotropy of the Co sublattice, and changes the easy magnetization direction of Gd3(Fe1−xCox)25Cr4 compounds from basal plane to easy axis. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.123247
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  • 5
    Electronic Resource
    Electronic Resource
    Structure and magnetic properties of TbMn6−xAlxSn6 compounds (1997)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 82 (1997), S. 760-763 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In the present work, the influence of the substitution of Al for Mn on the structure and magnetic properties of TbMn6−xAlxSn6 compounds was investigated. With increasing Al concentration the lattice constants a, c, and the unit-cell volume V decrease monotonically. The TbMn6−xAlxSn6 compounds exhibit ferrimagnetic ordering. The magnetic ordering temperature first decreases with increasing Al content up to x=1.5, then increases with further increasing Al content. As for the unsubstituted material, it was found that this series of compounds exhibits a second magnetic transition at lower temperature that is attributed to spin reorientation. The spin-reorientation temperature and the magnetic anisotropy field first decrease with increasing Al content, go through a minimum at x=0.5, then increase with further increasing Al content. The saturation magnetization decreases monotonically with increasing Al content. Two metamagnetic transitions are found in TbMn6Sn6 compounds, one at 0.44 T, and the other at about 0.16 T. With increasing Al content only one metamagnetic transition remains. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.365855
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  • 6
    Electronic Resource
    Electronic Resource
    Magnetic properties of Y(Fe0.8M0.2)11.3Nb0.7 compounds with M=Mn, Fe, Co, Ni, Al, and Ga : The 41st annual conference on magnetism and magnetic materials (1997)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 81 (1997), S. 5131-5133 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Structural and magnetic properties of Y(Fe0.8M0.2)11.3Nb0.7 compounds with M=Mn, Fe, Co, Ni, Al, and Ga have been investigated. The x-ray diffraction patterns of aligned samples show that, for M=Mn, Fe, Co, Ni, the compounds exhibit easy axis anisotropy while for M=Al and Ga the compounds have planar anisotropy. The anisotropy fields of Y(Fe0.8Mn0.2)11.3Nb0.7 increase monotonously with decreasing temperature. The influence on the Curie temperature and the magnetization due to the various substitutional atoms are discussed. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.365145
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  • 7
    Electronic Resource
    Electronic Resource
    Treatment of human hepatocellular carcinoma by fibroblast-mediated human interferon α gene therapy in combination with adoptive chemoimmunotherapy (1995)
    Springer
    Journal of cancer research and clinical oncology 121 (1995), S. 457-462 
    Details
    ISSN: 1432-1335
    Keywords: Interferon ; Gene therapy ; Fibroblast ; Hepatocellular carcinoma ; Immunotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The therapeutic effect of the fibroblast-mediated human interferon (IFNα) gene therapy in combination with interleukin-2 (IL-2) activated killer cells (AK)/doxorubicin (i.e., adoptive chemoimmunotherapy) on nude mice bearing the human hepatocellular carcinoma (HCC) was investigated. A fibroblast cell clone (NIH3T3-IFNα+) secreting 1024 U/ml human IFNα was obtained from 14 positive clones by BMGNeo-INFα DNA transfection, G418-resistant selection, limiting dilution and assay of IFNα activity. After i.p. implantation of NIH3T3-IFNα+ encapsulated into collagen, serum human IFNα activity could be detected from 12 h to day 15 with a peak at 72 h. AK were prepared from human peripheral mononuclear cells costimulated in vitro by IL-2 and inactivated human SMMC 7721 HCC cells. When the NIH3T3-IFNα+ cells were i.p. implanted into the HCC-bearing nude mice, the grown of HCC was inhibited and the survival time of the mice was extended. The growth of HCC was inhibited more obviously when AK was i.v. injected and IL-2 was i.p. injected after the NIH3T3-IFNα+ cells had been implanted. The best therapeutic effect was achieved when NIH3T3-IFNα+ cells were used in combination with IL-2/AK/doxorubicin. All these results suggested that the fibroblast-mediated human IFNα gene therapy could be used to treat the human hepatocellular carcinoma effectively and that when used in combination with IL-2-based adoptive chemoimmunotherapy, the therapeutic effect would be better.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01218361
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  • 8
    Electronic Resource
    Electronic Resource
    Involvement of MHC class I molecule and ICAM-1 in the enhancement of adhesion and cytotoxic susceptibility to immune effector cells of tumor cells transfected with the interleukin (IL)-2, IL-4 or IL-6 gene (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 602-608 
    Details
    ISSN: 1432-1335
    Keywords: Key words Interleukin-2 ; Interleukin-4 ; Interleukin-6 ; Gene transfection ; Melanoma cells ; Adhesion ; Cytotoxicity ; MHC class I ; ICAM-1 ; Effector cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate the molecular and cellular mechanisms involved in the reduced tumorigenicity and increased immunogenicity of interleukin-2 (IL-2)-, IL-4- or IL-6-gene-transfected B16 melanoma vaccine, we have analyzed the functional and phenotypic properties of these genetically engineered melanoma cells in the present study. The cytokine-gene-transfected B16 melanoma cells showed stronger adhesion to the lymphokine-activated killer (LAK) cells or cytotoxic T lymphocytes (CTL), and higher sensitivity to cytotoxicity of LAK cells or CTL. Using fluorescence-activated cell sorting analysis, we found that both MHC class I and ICAM-1 expression were increased after IL-2, IL-4 or IL-6 gene transfection. The increased level of MHC class I and ICAM-1 expression seems to be responsible for the high sensitivity of these gene-transfected B16 cells to LAK or CTL cytotoxicity because anti-(MHC class I) or anti-ICAM-1 mAb could inhibit the adhesion and cytotoxicity increment simultaneously. The CTL induction was partly inhibited by anti-ICAM-1 mAb and was completely blocked by anti-MHC class I mAb. These results suggested that the decreased tumorigenicity of IL-2-, IL-4-, and IL-6-gene-transfected B16 melanoma cells may be partly due to the increased sensitivity to effector cell cytotoxicity mediated by increased expression of ICAM-1 or MHC class I molecules on the tumor cell surface after cytokine gene transfection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004320050112
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  • 9
    Electronic Resource
    Electronic Resource
    Late Cenozoic magnetostratigraphy (11 – 0 Ma) of the Dongshanding and Wangjiashan sections in the Longzhong Basin, western China (1997)
    Springer
    Netherlands journal of geosciences 76 (1997), S. 121-134 
    Details
    ISSN: 1573-9708
    Keywords: reversals ; Miocene ; Pliocene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract A paleomagnetic study of the 510-m-thick Wangjiashan section of Late Miocene and Pliocene terrestrial sediments reveals a fairly complete reversal record with ages from 11 to 1.8 Ma. The magnetostratigraphy of the Dongshanding section, located nearby, reveals a partially overlapping reversal record with ages from 2.2 to 0 Ma, and facilitates correlation of the Wangjiashan section with the global polarity time scale. A new stratigraphic division of the Wangjiashan section replaces the name Linxia formation by five new formation names, based on lithologic variation and mammalian fossil finds. The new formations and their magnetostratigraphically determined ages are: Dongshan Formation (c. 1.75–2.6 Ma), Jishi Fm. (c. 2.6–3.6 Ma), Hewangjia Fm. (4.5–6.0 Ma), Liushu Fm. (6.0–7.6 Ma), and Dongxiang Fm. (7.6–c. 12 Ma). The Neogene stratigraphy and fossil mammals suggest that the nearby part of the Tibetan Plateau experienced a persistent denudation during the Late Miocene and Early Pliocene, but that it was uplifted more rapidly at about 3.6 Ma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003153717799
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  • 10
    Electronic Resource
    Electronic Resource
    NMR spectra of polyimides: Interpretation and local chain motion study (1996)
    Weinheim : Wiley-Blackwell
    Macromolecular Chemistry and Physics 197 (1996), S. 651-666 
    Details
    ISSN: 1022-1352
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Three kinds of high-performance polyimides 1 (poly(ketone-imide) PKI), 2 (poly(ether-imide) PEI) and 3 (poly(oxy-imide) POI) were studied using nuclear magnetic resonance (NMR). The NMR spectra of the polyimides were assigned according to the comprehensive consideration of the substitution effect of different substituting groups, viz. distortionless enhancement by polarization transfer (DEPT), no nuclear Overhauser effect (NNE), analysis of relaxation time, and two-dimensional correlated spectroscopy (COSY) techniques. The structural units of these three polyimides were determined. Carbon-13 and proton relaxation times for PEI and PKI were interpreted in terms of segmental motion characterized by the sharp cutoff model of Jones and Stockmayer (JS model) and anisotropic group rotation such as phenyl group rotation and methyl group rotation. Correlation times for the main-chain motion are in the tens of picosecond range which indicates the high flexibility of polyimide chains. Correlation times for phenyl group and methyl group rotations are more than 1 order of magnitude lower and approximately 1 order of magnitude higher than that of the main chain, respectively.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1996.021970220
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