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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 79 (1984), S. 21-26 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of salinity stress on biomass yield, photosynthetic O2 evolution and nitrogenase activity were investigated using axenic cultures of Nodularia harveyana (Thwaites) Thuret originally isolated from a salt marsh at Gibraltar Point, Lincolnshire, UK in 1971 and studied in this laboratory in 1983. Biomass yields, as chlorophyll a per culture, were highest in the 0 to 100% seawater (0 to 35‰ sea salt) range with negligible growth in 200% seawater; growth on NH 4 + was greater than on N2 and NO 3 - , which did not differ significantly from each other. In short-term experiments, photosynthetic O2 evolution remained high at salinities up to 150% seawater (52.5‰ sea salt); nitrogenase activity remained high at salinities up to 100% seawater (35‰ sea salt). The major internal low molecular weight carbohydrate which accumulated in response to increased salinity was sucrose, the levels of which fluctuated markedly and rapidly in response to salinity change.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 11 (1988), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. The solutes accumulated by cyanobacteria in response to hyper-osmotic stress include Na+, K+, sucrose, trehalose, glucosyl-glycerol, glyeine betaine and glutamate betaine. The compatibility of several of these solutes with glutamine synthetase activity has been examined using cell-free extracts from a range of freshwater, marine and halotolerant cyanobacteria. All of the solutes tested were compatible with (i.e. non-inhibitory to) enzymic activity at physiological concentrations and the results demonstrate a rank order of compatibility which correlates with the concentrations at which the organic solutes occur in cyanobacteria, i.e. glycine betaine 〉 polyol-derivatives 〉 disaccharides and with the upper salinity limit for growth. The protection against inhibition by NaCl (halo-protection) afforded by these solutes to enzymic activity was also examined. Only glycine betaine was found to exert a significant halo-protective effect and this may be explained by differences in the mechanism of compatible solute function between small charged molecules and sugars/polyols.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 13 (1994), S. 43-48 
    ISSN: 1476-5535
    Keywords: Milbemycin ; Fructose ; Starch ; Carbon:nitrogen ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Milbemycin production byStreptomyces hygroscopicus RB4569D was examined in media containing different carbohydrates. Total milbemycin titer could be increased by substitution of fructose for glucose and by selection of the appropriate starch type. Total titer could be further enhanced by increasing the concentration of fructose and/or starch in the medium. Rates of carbohydrate utilization were shown to be independent of their initial concentration and increased titers in high carbohydrate media were shown to be due to a prolonged production phase rather than an increased accretion rate. The pattern of individual milbemycin components was governed by the carbon:nitrogen ratio of the medium rather than carbohydrate concentration and there was a critical C:N ratio below which no milbemycin was produced.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 16 (1996), S. 295-300 
    ISSN: 1476-5535
    Keywords: milbemycin ; inoculum development ; seed stage fermentation ; Streptomyces hygroscopicus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Fermentation development for improved culture productivity can be achieved in a number of ways. Conventional approaches usually concentrate initially on optimisation of the final stage fermentation. However an understanding of the seed stage and its further development can lead to an improvement in final stage productivity. A significant increase in the production of milbemycin VM44866 byStreptomyces hygroscopicus was achieved by manipulation of several factors associated with the seed stage fermentation. Juvenile seeds and seed media containing reduced levels of carbohydrates overcame the detrimental effects of passaging and seed age associated with the standard (control) process. The effect of final stage inoculum level was seed medium-dependent and seed fermentation incubation temperature also affected subsequent milbemycin VM44866 production. These findings were extended to a second milbemycin-producing strain and these results have demonstrated the potential benefits of seed stage optimisation for improved final stage production.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 163 (1985), S. 424-429 
    ISSN: 1432-2048
    Keywords: Cyanobacteria ; Glucosyl-glycerol ; Osmotic adjustment ; Spirulina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The filamentous cyanobacterium Spirulina platensis has been examined for salt tolerance and osmotic adjustment. Salinities up to 150% seawater had little effect on growth yield or photosynthetic O2 evolution; higher salinities were markedly inhibitory. Osmotic adjustment was achieved by the intracellular accumulation of the low-molecular-weight carbohydrate glucosyl-glycerol in response to increased external salinity: in fullstrength (100%) seawater glucosyl-glycerol accounted for approximately 5.0% of the dry weight of the cyanobacterium. Trehalose was also present, particularly in cells at low salt concentration, and in 50% seawater medium accounted for up to 1.0% of the dry weight of the cyanobacterium. For cells grown in 100% seawater the ratio of trehalose to glucosyl-glycerol varied with temperature: at 37°C trehalose comprised 31% (w/w) of the low-molecular-weight carbohydrates while at 20°C only 9% of the total was trehalose. When subjected to hypo-osmotic shock the intracellular concentration of glucosyl-glycerol decreased and this was mirrored by an increase in glycogen. An understanding of the osmotic adjustment of S. platensis has implications both for the mass culturing of this and other strains of Spirulina and possibly also for the quality of the harvested product.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5036
    Keywords: Blue-green algae ; Cyanobacteria ; Osmotic responses ; Salinity tolerance ; Spirulina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Photosynthetic, prokaryotic blue-green algae (cyanobacteria) occur in a wide range of natural habitats of diverse ionic composition and as such, represent an important source of biological material for biosolar energy conversion programs using saline water. The gasvacuolate, filamentous Spirulina is grown in ‘seminatural’ culture in Lake Texcoco, Mexico, as a major source of single-cell protein for animal nutrition. Pilot-scale trials in other areas of the world have also demonstrated the suitability of blue-green algae, including Spirulina, for growth under brackish conditions. The carbohydrate accumulation profiles of blue-green algae differ in isolates from freshwater, marine and hypersaline habitats, with a trend towards sucrose or trehalose accumulation in stenohaline freshwater strains grown in media containing NaCl, while euryhaline and marine forms frequently accumulate glucosylglycerol. Many halotolerant isolates from hypersaline habitats accumulate glycinebetaine in response to osmotic stress. This knowledge may provide scope for future improvement in the N2 fixation rates of blue-green algae in saline media, using betaine-accumulating N2-fixing strains in preference to other, saltsensitive isolates.
    Type of Medium: Electronic Resource
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