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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 4 (1984), S. 169-181 
    ISSN: 0886-1544
    Keywords: cytoskeleton ; motility ; cell spreading ; epithelial cells ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Reorganization of intermediate filaments during cell spreading is examined by immunofluorescence, electron microscopy, and time-lapse video microscopy. A juxtanuclear cap, believed to correspond to the intermediate filament distribution center, was observed to be spatially related to the organization of the intermediate filament network as cells spread. A keratin cap was observed, which appeared spontaneously in motile PtK1 cells. Cap formation may be a consequence of retraction of intermediate filaments from the cytoplasm as cells move. The position of this juxtanuclear cap is related to the direction of movement, located on the side of the nucleus near the advancing edge of the cell. As the cell spreads, the cap disappears as the keratin filament network returns to the cytoplasm. Evidence presented here is consistent with the hypothesis that the distribution center mediates keratin filament organization during cell shape change.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The luminal and glandular epithelia of the rat uterus during delayed implantation have been studied with the electron microscope. The tall columnar cells of the luminal epithelium are characterized by a distinct zonation of organelles. On the free surface there are short microvilli and occasional cytoplasmic extrusions, with terminal bars joining adjacent cells at their apical margins. Beneath the free surface are numerous pinocytotic vesicles and mitochondria. Above the nucleus are a prominent Golgi apparatus with large dilated vacuoles, and numerous dense inclusion bodies. Large clusters of lipid droplets and aggregations of mitochondria occupy the basal zone of the cell.The apical portion of the gland cell has fewer pinocytotic vesicles and the Golgi is smaller than in the luminal epithelial cells. Dense bodies are numerous above the nucleus. The basal portion of the gland cell is devoid of lipid, but there are often one or more enlarged mitochondria. Although a dense PAS+ substance fills the lumina of the glands, there is no obvious evidence of secretory activity in the glands during delay.The relationships of the epithelia to the environment of the blastocysts during delay is discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 177 (1973), S. 225-241 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Electron microscopic examination of developing myofibrils in regenerating skeletal muscle of Rana pipiens tadpole tails reveals that sarcoplasmic reticulum (SR) is in regular and close association with Z-lines of young sarcomeres before transverse tubules have formed alongside the myofibrils. SR is also associated with M-lines of young sarcomeres but on a less regular basis than with Z-lines. Counts of the number of contact sites of SR with Z-lines, M-lines and combined A- and I-bands of longitudinally sectioned sarcomeres of young myofibrils confirms the visual impression that the SR associates with a relatively much greater frequency with Z-lines and M-lines than with other regions of the sarcomere. This suggests the formation of a specific bond between SR and Z- and M-lines.Serial transverse sections of developing myofibrils were examined in order to determine whether Z-lines displayed SR association at all stages in their formation. Of fifty discrete Z-lines that were examined, only four smaller Z-lines appeared to lack SR association. An examination of a comparable number of more diffuse Z-bodies representing an earlier stage of Z-line formation suggests that no more than one-half of these Z-bodies were accompanied by SR. These observations support the conclusion that SR does not initiate formation of Z-line precursor elements, but rather associates with Z-lines as they condense from more diffuse Z-bodies.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 124 (1969), S. 1-29 
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The abdominal intersegmental molting muscles of Rhodnius prolixus undergo a repeated differentiation and dedifferentiation correlated with the molting cycle of the insect. In the dedifferentiated phase, the muscle fibers lack myofibrils but contain a few bundles of 50-60Å filaments associated with amorphous dense plaques on the sarcolemma. Differentiation of the muscle fibers begins when a starved Rhodnius nymph takes a blood meal. The first morphological signs of differentiation at ten hours after feeding are a dispersal of ribosomes from the nuclear envelopes and a formation of polysomes in the sarcoplasm. The initial myofilaments appear within 10 to 15 hours after the blood meal and are preferentially deposited in the cortices of the muscle fibers in association with the 50-60Å filament bundles and the dense plaques on the sarcolemma. Striated myofibrils are present after five days of differentiation. Developmental continuity between Z-band segments and sarcolemmal dense plaques is suggested. The formation of cross-bridges between thick and thin myofilaments appears to be the primary mechanism of myofibril organization. Disruption of microtubules with colchicine does not significantly alter myofilament deposition and organization during early stages of differentiation. Dyads, consisting of smooth sarcoplasmic reticulum and invaginations of the sarcolemma, are present in the dedifferentiated muscle fiber, and increase in number as differentiation proceeds.
    Type of Medium: Electronic Resource
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